ABSTRACT
To assess the histopathological diagnosis made on the cases sent for second opinion to the Department of Histopathology. A descriptive study. Department of Histopathology AFIP, Rawalpindi, Pakistan from July 2010 to April 2011. During the study period, 142 cases were received for second opinion by the department. The demographical details of the patients were entered in a profroma. After initially seeing the H and slides, immunohistochemical and special stains were applied where required. The initial diagnosis and review diagnosis were then analysed. During the study period, 142 cases were analysed, 81 were male and 61 female patients. There was wide age range, from 2 months to 90 years. Out of the total 22 [15.5%] were reviewed for benign conditions and 120 [84.5%] were malignant. Majority of cases were from lymphoreticular system. In 72 [50.7%] cases diagnosis was changed on review out of which 9 were benign conditions and rest malignant. Out of the 63 malignant 27 cases and 3 out of 9 benign cases, were those where change in diagnosis was such that it changed the treatment pattern. In 12 cases the review diagnosis was changed from benign to malignant and vice versa. Getting second opinion on surgical biopsy material is very important part of treatment, particularly in our set up, where all the laboratories are not fully equipped.
ABSTRACT
To histomorphologically analyze the oral white lesions. Descriptive, cross-sectional study. Department of Histopathology, Armed Forces Institute of Pathology [AFIP], Rawalpindi. From May 2011 to May 2012. A total of 54 patients presenting with oral white lesions were included. Age, gender, site and size was recorded. The biopsy sample was fixed in 10% formalin and after standardized processing, slides were prepared, stained by Hematoxyline and Eosin, with special stains when required. The histopathological diagnosis of lesion was recorded. Mean and standard deviation were calculated for quantitative variable. Frequency and percentages were calculated for qualitative variables. Out of total 54 patients 28 were male and 26 females. The age range was 17-80 years with mean age 49.70 years. Majority of the patients were between 50-60 years. Buccal mucosa was affected in majority of the cases [63%], followed by lip mucosa and tongue. Histologically Lichen Planus was the most common lesions [33%], followed by non specific inflammation [14.8%], keratosis without dysplasia [11%], keratosis with dysplasia [7.4%], Pemphigus Vulgaris [6%] and Squamous cell carcinoma [1%].Lichen Planus is the most common cause of white lesion in our set up. Biopsy of an intact epithelial lesion should be considered if the lesion does not resolve in due course of time
ABSTRACT
To evaluate the BerEP4 stain positive cases of basal cell carcinoma in our population [BCC]. A non-interventional descriptive study. Military Hospital and Armed Forces Institute of Pathology [AFIP] Rawalpindi from 1[st] January 2009 to 31[st] August 2009. Patients who reported to the skin department of Military Hospital Rawalpindi with clinical impression of BCC were biopsied. Only those cases which were easily diagnosed on Haematoxylin and Eosin [HandE] were included. All such sections were then subjected to BerEP4 immune marker and the intensity of staining was noted. In addition, 11 cases of straight forward squamous cell carcinoma [SCC] were also stained with BerEP4, which served as controls. The study group [BCC cases] included 17 males [59%] and 12 females [41%], who were histologically diagnosed as BCC. All such cases were subjected to BerEP4 immune staining. All the tumors showed positive staining, the intensity/staining pattern was however variable. Twenty-two out of twenty-nine cases showed diffuse [75.9%], while 7 out of 29 cases showed partial [24.2%] staining; irrespective of the histological subtype and site of tumor. All the SCCs were negative for BerEP4 staining. BerEP4 was positive in 100% of the cases. Its intensity was however variable. Nevertheless, it must be used with confidence in all difficult to diagnose cases of BCC, especially when it is difficult to differentiate from SCC