Role of E-cadherin in differentiation between squamous and basal cell carcinoma

Some type of basal cell carcinoma [ex Keratotic variant] is often misdiagnosed as squamous cell carcinoma and basal cell carinoma. The objective of this study was to determine the role of E-cadherin in differentiation between Squamous and basal cell carcinoma. The sampling was performed by simple method. Entry criteria to the study included 2 groups of 34 primary, untreated BCCs and SCCs each from a different patient. They were surgically removed and the diagnosis was confirmed by histopathology. The stained sections were scored by levels of expression and intensity of E-cadherin. Distribution of E-cadherin staining was more in BCC than SCCs [P=0.006] but the decreased staining intensity of E-cadherin in SCC compared with BCC was not statistically significant [P=0.056]. When the results [score of distribution and intensity of E-cadherin] were added together, the acquired E-cadherin index in a statistical analysis was meaningful to differ between SCC and BCC [P=0.002]. Our study showed that E-cadherin distribution and index but not intensity is lower in SCC cells than BCC cells and it can justify the cause of metastasis in SCC. However, this is ambiguous to use it for differentiating of aggressive forms of BCCs from SCCs, practically

effect of epstein-barr virus latent membrane protein-1 status on outcome of patients with classic hodgkin's lymphoma

This study determines the expression of latent membrane protein-1 [LMP- 1] in lymph nodes of patients with classic Hodgkin lymphoma [cHL] and its role in patients' survival. Specimen of 98 patients who had cHL was chosen and the samples were immunohistochemically stained with LMP-1 and Overall disease-free survival [DFS] was measured from the date of complete remission until the Relapse was confirmed by another lymph node biopsy. We investigated the expression of LMP-1 in outcome of patients with cHL. LMP-1 was detected in RS cells of cHL in twenty five out of ninety eight [25.5%] patients. LMP-1 expression was significantly more frequent in mixed cellularity, 46.7%, than nodular sclerosis, 15.4% [P= 0.005]. Patients with EBV-positive tumors had fewer DFS [22.6 vs. 25.9 mo], but the difference was not statistically significant [p=0.06]. Although in our study there is no relationship between age and LMP-1 expression. LMP-1 expression is associated with statistically different DFS in treated patients with cHL

effect of estrogen on wound healing in rats

Cutaneous wound healing involves multiple cooperative molecular processes such as inflammation, angiogenesis, wound contraction, granulation tissue formation, reepithelialization, and matrix deposition. We studied the effects of topical estrogen on wound healing in male rats. This experimental study was done on 40 male rats. A circular wound with a diameter of 2cm was induced on each rats right flank. Twenty rats received topical estrogen [case group]. And twenty other rats received placebo [control group]. After the 5th, 10th, 15th, 30th, 35th, 40th and 45th days, healing process was compared between the two groups. On the 10th and 20th days the total healing surface in the case group was about 89.9% and 100% respectively and 75% and 98,4% [p<0.05 and P>0.05]in the control group. Microscopic views revealed the formation of epithelial layer and hair follicles, progressive angiogenesis without scarring in case group. But neither hair follicles nor complete epithelial layer in the control group Topical estrogen administration results in significant progress of cutaneous wound healing, leaving no scar or crust formation. Topical estrogen administration accelerates healing without changing plasma estrogen level and can minimize the probable wound complications

Determination of P53 expression in basal cell carcinoma tissues and adjacent nontumoral epidermis from sun-exposed areas of the head and neck

This study determines the expression of P53 protein and the intensity of immunoreactivity in basal cell carcinoma in comparison with the adjacent nontumoral epidermis in sun-exposed areas of the head and neck regions. The mean age of immunoreactivity in tumoral and adjacent nontumoral epidermis is also determined. This descriptive-analytical study was performed retrospectively over a 5-year period on 150 basal cell carcinoma cases in the Pathology Department of Alzahra Hospital in Isfahan. Proper quality paraffin blocks were chosen for immunohistochemical staining for P53 through the immunoperoxidase method. The intensity of immunoreactivity was graded. The age of the patients was also recorded. Positive P53 immunoreactivity was observed in 123 basal cell carcinoma tissues [82%] and in 117 adjacent nontumoral epidermis [78%] [P = 0.38]. The frequency of severe immunoreactivity in tumoral tissue and in adjacent nontumoral epidermis was 46% and 32%, respectively [P = 0.046]. The mean age of P53 expression was 66.2 years for tumoral tissue and 66.1 years for nontumoral epidermis. The mean age of those who did not express P53 was 52.6 and 55.9 years for the tumoral and nontumoral epidermis, respectively [P < 0.001]. No significant difference was detected between P53 immunoreactivity in tumoral tissue and adjacent nontumoral epidermis. Intensity of P53 immunoreactivity was greater in tumoral specimens. Comparison of mean ages showed a significant difference between P53- expressing and non-P53-expressing groups