ABSTRACT
Background: The expression of MT genes in animal's body is rapidly elevated in response to metal and agents which cause oxidative stress and/or inflammation. The MTF-1 plays an essential role in regulating transcription of MT gene in response to metal ions and oxidative stress. Metalloviatamin complex was found to exhibit anti-tumor and anti-inflammatory activity, cytoprotective effect and reduces oxidative stress
Objectives: The study was designed to evaluate effects of a daily dose of copper nicotinate complex [CNC] on metallothionein-III [MT-III] and metal-responsive transcription factor -1 [MTF-1] expression during 4-dimethylaminoazobenzene [DAB] exposure. Methods: Ninety rats were divided into five groups. Group I rats were fed on standard diet and was considered the control group; group II rats were fed on standard diet containing DAB [0.06g/100g diet daily] for six months; group III rats received CNC dissolved in saline solution [1mg/kg body weight daily] for six months; group IV rats were pretreated with CNC one month before DAB; group V rats were post treated with CNC after one month of starting feeding on DAB. MT-III and MTF-1 genes expression was assayed by PCR. Renal histopathological changes were examined by light microscopy
Results: Genes expression in all groups was statistically high at all time intervals as compared with control group, while it was decreased in groups III, IV and V as compared to cancer group [II]. In group IV and V, the genes expression was statistically increased as compared with group III. MT expression in control group declined with age, while it was higher at 6th month than 2nd and 4th month in group II. MTF-1 expression was increased at 4[th] month followed by decrease at 6[th] month in all studied groups. These results were confirmed by histopathological change. There was an increase in pyknotic and necrotic nuclei in tubular epithelial cells and a mild dilatation of renal tubules
Conclusion: Scavenging ROS during DAB-induced oxidative stress may be the major role of MT. CNC causes a partial improvement in the genes expression as well as renal tubules, so CNC may be a promising candidate used for protection against oxidative stress
ABSTRACT
Diazinon are widely used as pesticides in agriculture. So, the current work aimed to investigate the effects of diazinon exposure on some physiological aspects, histopathological changes and histochemical acetyl- cholinesterase in red Baladi rabbits. Seventy-two red Baladi bucks were distributed into three groups, the rabbits of the first group were dipped into tap water and served as control group. The rabbits of the second and the third groups were dipped in diazinon at concentrations of 0.6 mg [DLC] and 3 mg [DHC] dissolved in one litter of water, respectively for 10 sec. This step was repeated after 10 days. The animals were sacrificed by jugular vein incision after 0, 1, 3, 7, 15 and 21 day following the second dipping of rabbits in diazinon. In whole blood, diazinon decreased rabbits's RBC's [P<0.01] and Hb [P<0.05], while MCV and MCH were elevated [P<0.01] for both tested concentrations, but PCV values were increased only [P<0.01] in the DHC group. In plasma TP was decreased [P<0.01] in both tested concentrations, however cholesterol was increased [P<0.01] in the DLC and DHC groups. Meanwhile hydroxylamine and nitrite were increased only in the DHC treated group. Liver body weight ratio and cytochrome P-450 were decreased [P<0.01] in both tested concentrations, while microsomal protein was increased [P<0.01] in both concentrations. RBC's, PCV and microsomal protein were increased [P < 0.01] by the days of treatment. Meanwhile, MCV, MCH, MCHC, liver body weight ratio and cytochrom P-450 were decreased [P <0.01] by the days of treatment. There was no definite trend with days of treatment for WBC's, Hb, TP, cholesterol, hydroxylamine and nitrite. There was a highly significant effect of concentration X day interaction [P<0.01] on all tested parameters. Histopathological changes of liver, kidney and brain were observed after DHC dipping. Glycogen content was decreased in liver and increased in kidney Bowman's capsule. Furtheremore, the AChE activity was inhibited in brain tissue and decreased in liver and gradually increased in kidney glomeruli cells. Exposure of animals to diazinin caused extensive changes in physiological, histochemical and histopathological parameters, the kidney and brain were highly affected by the diazinon exposure when compared with liver. Diazinon lead to negative response on animal performance
Subject(s)
Animals, Laboratory , Rabbits , Acetylcholinesterase/blood , Liver/pathology , Kidney/pathology , Brain/pathology , Histology , Cholinesterase Inhibitors , InsecticidesABSTRACT
Four-five weeks old rats were included in this study and divided into two experimental groups received single or split doses of praziquantel [PZQ] as well as two control groups, one infected untreated and the other normal healthy rats. The effect of infection on rat growth as well as the jejunal and duodenal architecture was histopathologically studied after H and E staining. The jejunal ultrastructure was examined by SEM and TEM. The effect of PZQ was evaluated using the same techniques. It was observed that infected as well as infected treated animals had less weight than the healthy controls. The intensity of infection decreased gradually after treatment. The cure rate was 100% after split dose and 80% after a single dose. Altered villus height and cryptic depth were the characteristic changes in the architecture of the duodenum and jejunum, more pronounced in the latter. The split dose of PZQ revealed a more improvement of the histopathological findings than the single dose. By SEM, circular imprints representing defects in the villi were observed in the jejunum. By TEM deformation of microvillar architecture was observed together with organellar changes in the RER and the mitochondria after PZQ treatment