ABSTRACT
Chronic kidney failure is associated with dyslipidemia and accelerated atherosclerosis. To study lipidic metabolism alterations in patients with chronic kidney failure on hemodialysis. The study interested 45 hemodialysis patients with a mean age of 49.04 +/- 15.92 years old and 45 healthy controls. A blood sample was collected from each patient and control to measure total cholesterol, triglycerides, HDL- cholesterol, LDL-cholesterol, apolipoproteins AI and B100, lipoprotein [a] and C Reactive Protein. A significant increase of serum triglycerides [p= 0.002], lipoprotein [a] [p = 0.001] and C Reactive Protein [p = 0.008] was observed in patients when compared with healthy controls. A significant decrease of serum total cholesterol [p=0.01], HDLcholesterol [p<0.001], LDL-cholesterol [p=0.005] and apolipoprotein AI [p<0.001] was also observed in patients. Disorders of lipidic metabolism are frequent in hemodialysis patients. These alterations can lead to cardiovascular disease in uremic patients
ABSTRACT
To investigate the frequencies of C677T polymorphism in MTHFR gene and G80A polymorphism in RFC gene in obese and no obese Tunisian children and to assess their relation with homocysteine [tHcy], folate and vitamin B12 levels. We have studied 31 obese compared to 22 no obese children. tHcy was assessed by fluorescence-immunoassay ; folate and vitamin B12 by radioimmunoassay. C677T and G80A mutations were detected using pyrosequencing. There were no differences in tHcy levels between obese and no obese, [10,34 +/- 4,86micro moll/l vs11,00 +/- 4,26micro moll/l]. We found no difference for the allelic frequencies of the C677T polymorphism [29.03% vs 30.95%] and of the G80A polymorphism [64.52% vs 59.52%]. Mean levels of tHcy, folic acid and vitamin B12 were not significantly different according to MTHFR and RFC genotypes. We demonstrated no difference in tHcy, folates, vitamin B12 levels and allelic frequencies of C677T and G80A polymorphisms in MTHFR and RFC genes between obese and no obese Tunisian children. These two polymorphisms don't seem to have any impact on homocysteine, folate and vitamin B12 status in the two populations
ABSTRACT
Glutathione S-transferases [GST] play a vital role in cellular defense against environmentally toxic compounds. These enzymes present a genetic deletion polymorphism, which varies with ethnicity. To evaluate the frequencies of homozygous deletion of GSTM1 and GSTT1 genes in Tunisian population. On the basis of multiplex PCR protocol, the frequency of the deleted genotypes of GSTM1 and GSTT1gees was evaluated on 145 healthy Tunisian subjects. We found that 34.6% of the individuals had the GSTM1 null genotype, 16.6% had the GSTT1 null genotype and 4.82% had a double deletion of both GSTM1 and GSTT1. The distribution of GSTM1 null in Tunisians is rather in the range of black populations and is lower than that reported in Asians, Arabs and Caucasians. However the frequency of GSTT1 null is the range of several populations studied except Asians. The double deletion frequency seems lower than that described in different populations
ABSTRACT
Thyroid carcinomas represent the most common endocrine malignancy, and several biological markers are proposed according to the different types of this cancer: for papillary cancer, thyroglobulin constitutes an excellent prognostic factor and rearrangements of ret oncogene can be useful in diagnosis. In sporadic medullary carcinoma, calcitonin is a diagnosis marker of choice, and coupled with ACE, can prevent relapse. Regarding familial medullary carcinoma, mutation screening in ret oncogene leads to early detection of new cases
Subject(s)
Humans , Biomarkers , Carcinoma, Papillary , Thyroglobulin , Carcinoma, Medullary , Calcitonin , Carcinoembryonic Antigen , Genetic Therapy , Proto-Oncogene Proteins c-retABSTRACT
Our prospective study included 41 patients, from 13 to 70 years old, and present a nasopharyngeal carcinoma confirmed histologically, during the period going from September 1999 to March 2000, and 45 healthy controls. A blood sample was collected from each patient before any treatment, as well as controls to measure serum LDH and its isoenzymes. Two groups of patients were selected after a period varying from 12 to 37 months with a mean of 29 months: 29 with favourable evolution, 12 with non favourable evolution. The mean serum total LDH and its isoenzymes values were significantly higher in patients than those in controls with values of variable p of 0,001 to 0,05. A significant correlation was found between ganglionnary extension and serum values of total LDH, LDH3 and LDH5. No significant difference were observed between the means serum total LDH before treatment and the clinical evolution of patients. Diagnostic contribution of total LDH is limited, by its ubiquitary character, but could constitute for LDH3 a good marker of the disease progression