Modulatory role of immunization with secretory-excretory products of schistosoma haematobium eggs on morbidity in infected hamsters

A variety of secretory-excretory products [SEPs] from different stages of Schistosoma have been identified to induce a level of host-protective immune responses with amelioration of morbidity. Identification of SEPs complex components can be expected to facilitate discovery of new therapeutic drug targets and new diagnostic markers for schistosomiasis control. The present study was undertaken to evaluate the possible anti-morbidity effects of SEPs of S. haematobium eggs twelve weeks after exposure to infection. The liver and intestinal tissues of infected hamsters were selected for evaluation as in the murine models the urinary bladder exhibits minimal morbidity in response to S. haematobium infection. The experimental design included three groups of 10 hamsters each; SEPs immunized group, infected immunized group and infected control group. Multiple small doses of purified S. haematobium eggs SEPs were injected intra-peritoneally, followed 2 weeks later with 2 booster doses at weekly intervals. Animals were infected with S. haematobium cercariae 1 week following last booster immunization dose. All animals were sacrificed 12 weeks PI. Assessment of the modulatory role of SEPs immunization including worm burden, tissues egg loads, oogram pattern and histopathological examination of liver and intestinal tissues were carried out. Total and subclasses of anti SEPs of S. haematobium eggs IgG, IgM, IgG2 and polyvalent immunoglobulins [Igs] were measured using indirect ELISA at weeks 3, 6 and 9 post-infection [PI]. Immunization with SEPs of S. haematobium eggs produced significant reduction in worm load [61.37%], reduction in tissue egg loads [54.85% and 41.57% for hepatic and intestinal ova, respectively]; decreased percent of immature stages and increase in the percent of dead ova in oogram pattern. Pathological examination also revealed significant reduction in number of hepatic granuloma [46.06%]. At 3, 6 and 9 weeks PI, the level of Igs especially IgG was significantly higher in the infected immunized group, compared to both control groups, reached a peak value at 6 weeks PI [1.6] and remained elevated till the end of the experiment with slightly decreasing tendency at 9 weeks PI [1.3]. This study could represent an immunization model as a trial to decrease severe morbidity of schistosomiasis haematobium which may be aggravated by serious sequels

con A purified hydatid glycoprotein fraction effectively diagnoses human hydatidosis

Diagnosis and quantification of Echinococcus granulosus in-fection in man and animal hosts are centralized to feasible con-trol this study included 93 serum sample, 25 sure positive hydatid cases confirmed surgically, 7 suspected cases diagnosed by indirect haemagglutination IHA and 41 cases other parasitic infection [15 S. mansoni, 8 fasciola, 7 Ascaris, 5 H. nana and 6 Ancylostoma] diagnosed by microscopic examination and were negative by ELISA and/or IHA for anti-hydatid antibody. Twen-ty negative serum samples served as healthy controls. Six types of hydatid fluid antigens [crude, host-free and Con-A purified] of human and camel origin were subjected to electrophoretic separ-ation [SDS-PAGE] and immunoblotting [EITB]. The anti-hydat-id IgG was detected in sera of the different groups for evalua-tion of sensitivity, specificity and diagnostic efficacy each type of antigens. Detection of circulating hydatid antigen [CAg] was performed using anti rabbit hyperimmune sera raised again-st Con-A purified either human or camel hydatid antigen. SDS-PGE revealed several bands ranging from 55-185- kDa with 10kD band shared by all antigens. The specific bands revealed by EITB for Con-A purified camel and human antigens were at 80, 110, 55, 110 kDa respectively. ELISA highest sensitivity [96.9%] was by using host-free Con-A purified glycoprotein fraction of human hydatied antigen. Highest specificity [98.4%] was reco-rded upon use of either Con-A purified camel or human antigen with 94.5% and 97.7 and diagnostic efficacy respectively. Detection of circulating antigen by polyclonal antibodies against Con-A purified human hydatid antigen revealed 91.8%specificity.

Epidemiological study of occupational accidents in Port Said: organization of the care in Port Said

There is a special centre for labour accidents which is a part of port-said Healthy insurance organisation [H.I.O] polyclinic in this centre. This centre received all cases of labour accidents in port-Said and referred to different especially in Mobera hospital. A special file to each new case is initated, up dated and kept in the centre. This study include 7761 patients were treated from occupational accident in Period 5 years [1-10-85 : 30-10-90]. All cases of occupational injuries were studied and classified according to the site and type of injurery. Contusion and sprains 3960 cases 51% superficial Wounds 1890 cases 24% fractures 8 11 cases 10.4% amputation 130 cases 1.7. Deaths 8 patients 0.1%. Eye injury 150 cases 2%, head injury 90 cases, 1.3% and disabilities 340 cases, 5.5%. Abdominal injuries 50 cases 66%