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Southeast Asian J Trop Med Public Health ; 1993 Sep; 24(3): 477-9
Article in English | IMSEAR | ID: sea-30623

ABSTRACT

The study describes a comparison between molecular hybridization using a non-radiolabeled, thymine-thymine (TT) dimerized synthetic oligonucleotide complementary DNA probe and electron microscopy for the detection of hepatitis E virus genome in bile. Spot hybridization with the TT dimerized probe was found to be more sensitive and specific compared to electron microscopy.


Subject(s)
Animals , Bile/microbiology , DNA, Complementary , DNA, Viral , Genome, Viral , Hepatitis E/microbiology , Hepatitis E virus/genetics , Microscopy, Electron/methods , Monkey Diseases/microbiology , Nucleic Acid Hybridization/methods , Oligonucleotide Probes , Pyrimidine Dimers , Sensitivity and Specificity , Time Factors
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