Effects of chronic caffeine administration on the platelets aggregation, blood coagulation and fibrinolytic activity in male rats

The effects of three caffeine doses [10, 20 or 40 mg/kg-1] given intravenously for seven consecutive days on platelets aggregation index, plasma fibrinogen and plasminogen levels, clotting time [CT], prothrombin time [PT] and activated partial thromboplastin time [APTT] were investigated in male albino rats. A decrease in PT and APTT was observed in the rats treated with the 10 mg dose, but no other changes were found in the rest of the studied parameters. The 20 and 40 mg doses caused a marked inhibition concentrations as well as prolonged CT, PT and APTT. The results suggest that long term administration of caffeine did not only inhibit the process of platelet aggregation, but also interfered with the intrinsic and extrinsic pathways of coagulations. The mechanisms by which caffeine caused these alterations might have been related to its phosphodiesterase inhibitory action as well as its effect on the liver function

In-vitro effect of pentoxifylline on sperm motility, seminal plasma CA cation content and transaminase activity

In this in vitro study, it was found that addition of 0.2 mg Pentoxifylline per ml of semen obtained from asthenozoospermic and oligoasthenozoospermic males induced a remarkable increase in the seminal plasma Ca[2] level [P < 0.0001], and also increased the activity of the seminal plasma glutamic oxalacetic transaminase [GOT] [P < 0.0001]. The increase in both Ca[2] level and GOT activity which were brought about by the action of this drug is assumed to be related to the increase in sperm motility from 50.07 +/- 20.83 [Mean +/- S.D.] to 63.33 +/- 21.78 [Mean +/- S.D.] and this increase was found to be highly significant [P < 0.0001]

In-vitro effect of pentoxifylline on sperm motility and seminal plasma phosphatases activity

From this in vitro study, it was found that the addition of pentoxifylline [a phosphodiestrase inhibitor] to the semen of asthenozoospermic and oligo-asthenozoospermic patients increased the percentage of motility of the sperms from 50.07 +/- 20.83 [mean +/- S.D.] to 63.33 +/- 21.78 [mean +/- S.D.] and this increase was found to be highly significant [p < 0.001]. The action of this drug was found to be optimal at a dose of 0.2 mg/ml of semen. Addition of 0.2 mg pentoxifylline to each ml of semen also resulted in increase of the activity of each of acid and alkaline phosphatase and this increase of the activity was found to be statistically significant at p < 0.0001. The increase in the percentage of motility of spermatozoa of subfertile patients after addition of pentoxifylline [0.2 mg/ml] is assumed to be related to the increased activities of both acid and alkaline phosphatase which are brought about by the action of this drug

Estimation of some seminal plasma enzymes of fertile and infertile Egyptian males

Four seminal plasma enzymes [acid and alkaline phosphatase, lactate dehydrogenase [LDH] and glutamic oxalacetic transaminase [GOT]] were estimated in 50 males [10 normal control and 40 infertile presenting with either azoospermia or oligozoospermia]. The results of this study show a marked decrease in the activity of alkaline phosphatase [p < 0.001] as well as LDH [p < 0.01] in the subfertile groups compared to normal control. There is no difference in the activity of acid phosphatase between the studied groups and the control. This study, also show no significant difference in the activity of GOT between normal control and azoospermic patients, but the activity of this enzyme decreases significantly [p < 0.0001] in oligozoospermia of both types compared to normal controls. Regarding the infertile groups, no difference was recorded between any of them as concerning acid and alkaline phosphatase activity. LDH activity is significantly higher [p < 0.01] in oligozoospermia compared to azoospermic group. On the other hand, GOT activity is significantly higher [p < 0.001] in azoospermia as compared to oligozoospermia of both types