efficacy of non cultured melanocytes and keratinocytes transplantation in treatment of generalized vitiligo

Objective: determine the efficacy and safety of the non-cultured melanocyte-keratinocyte transplantation procedure in the treatment of stable resistant generalized vitiligo. Design: A simpler and modified method based on that of Olsson and Juhlin [1998] was performed this method uses a shaved biopsy skin sample one tenth the size of the recipient area. The skin sample is incubated, and the cells are mechanically separated using trypsin-EDTA solution and then centrifuged to prepare a suspension. Cell suspension is then applied to the derma braded depigmented skin area, and a collagen dressing is applied to keep it in place

Patients: twenty one patients with stable generalized vitiligo of different sites and sizes were treated with NMKT and followed up for 6 months

Intervention: Autologous, non-cultured melanocyte keratinocyte cell transplantation

Main Outcome Measure: initial pigmentation was observed. Regimentation was graded as excellent with 75 to 100% pigmentation, good with 50% to 74% fair with 25 to 49%, and poor with <25% pigmentation, assessment by modified vitiligo area scoring index, color match ,patient satisfaction and adverse events were assessed

Results: 9.66% showed excellent response, 11.50 % showed good response, 40.38% showed fair response and 38.46 % with poor response. Average percent change in VASI was 24.56% +-33.71. The color matching was excellent to good in 65.4% and poor in 34.6% of lesions, 3.8% of patients only were very satisfied and complications were minimal. Limitations: Limitations include small sample size, lack of control group and short follow-up period

Conclusions: NMKT is an easy economic technique, which may be used in resistant areas of stable vitiligo. The smaller the size of the lesion and the longer the stability duration the higher the percentage of regimentation response obtained. Results tend to be better over the trunk and proximal limbs than elbows, knees and distal extremities. Complications are minimal the most common is post inflammatory hyperpigmentation of the donor area

Free radicals, total antioxidant status and interleukin-6 in seminal plasma of fertile and infertile men.

This work aimed to assess total antioxidant status, lipid peroxides [LPO], nitric oxide [NO] and interleukin-6 [IL-6] in seminal plasma of infertile men [n = 45] compared with fertile men [controls, n = 30]. The infertile men included asthenozoospermic [n = 17], asthenoteratozoospermic [n = 25] and oligozoospermic patients [n = 3]. The semen samples from fertile men showed antioxidant capacity at a higher value than equivalent samples from infertile men. Moreover, the inverse relationship between antioxidant capacity and lipid peroxidation in seminal plasma strongly suggested that impaired defenses play an important role in infertile disorders. Furthermore, the increased oxidative stress observed in infertile men may modulate the level of IL-6