Athens-like G6PD variant associated with chronic non-spherocytic hemolytic anemia found in Jordan

A G6PD variant associated with hereditary non-spherocytic hemolytic anemia is characterized. The variant which closely resembles Athens-like variant was found in a male member of a family of Palestinian origin. The enzymatic properties were; normal electrophoretic mobility, normal Km NADP + low Km G6PD, increased utilization of substrate analogues, moderate heat stability, and a biphasic pH curve with two peaks at pH 7.0 and 10.0.Athens-like variants resemble the Mediterranean variant of G6PD but can be readily distinguished by the rate of utilization of the substrate analogue deamino-NADP + [about twice less]