ABSTRACT
BACKGROUND: An association between arterial stiffness and osteoporosis has previously been reported. Therefore, we investigated the relationship between arterial stiffness, measured by brachial-ankle pulse wave velocity, and bone mineral density in a sample of healthy women undergoing routine medical checkup. METHODS: We retrospectively reviewed the medical charts of 135 women who had visited the Health Promotion Center (between May 2009 and December 2012). Brachial-ankle pulse wave velocity was measured using an automatic wave analyzer. Bone mineral density of the lumbar spine (L1-L4) and femur was measured by dual-energy X-ray absorptiometry. Metabolic syndrome was defined according to National Cholesterol Education Program-Adult Treatment Panel III criteria, using body mass index >25 kg/m2 instead of waist circumference >88.9 cm. RESULTS: Pearson's correlation analysis revealed significant inverse relationships between pulse wave velocity and bone mineral density of the lumbar spine (r=-0.335, P<0.001), femur neck (r=-0.335, P<0.001), and total femur (r=-0.181, P=0.04). Pulse wave velocity showed the strongest association with age (r=0.586, P<0.001). Multiple regression analysis identified an independent relationship between pulse wave velocity and lumbar spine bone mineral density in women after adjusting for age, metabolic syndrome, body mass index, smoking status, alcohol intake, and exercise (r=-0.229, P=0.01). CONCLUSION: This study confirmed an association between arterial stiffness and bone mineral density in women.
Subject(s)
Female , Humans , Absorptiometry, Photon , Body Mass Index , Bone Density , Cholesterol , Education , Femur , Femur Neck , Health Promotion , Osteoporosis , Pulse Wave Analysis , Retrospective Studies , Smoke , Smoking , Spine , Vascular Stiffness , Waist CircumferenceABSTRACT
BACKGROUND: Association of arterial stiffness and osteoporosis has been previously reported in women. However, this association is still controversial for men. Therefore, we investigated correlation of arterial stiffness and osteoporosis by measuring brachial-ankle (ba) pulse wave velocity (PWV) and bone mineral density (BMD). METHODS: We reviewed medical charts of 239 people (women: 128, men: 111) who visited the Health Promotion Center, retrospectively. ba-PWV was measured by automatic wave analyzer. Lumbar spine (L1-L4) BMD and femur BMD were measured by dual energy X-ray absorptiometry. Metabolic syndrome was based on the National Cholesterol Education Program (NCEP)-Adult Treatment Panel (ATPIII) definition. Body mass index (BMI)>25 kg/m2 was used instead of waist circumference. RESULTS: In Pearson's correlation analysis, PWV and femur BMD (Neck, total) had a significant inverse relationship in men (r=-0.254, P=0.007; r=-0.202, P=0.034). In women, PWV and the L-spine, femur (Neck, total) had a significant inverse relationship. (r=-0.321, P<0.001; r=-0.189, P=0.032; r=-0.177, P=0.046) Age and PWV showed the greatest association in both men and women (r=0.46 P<0.001; r=0.525, P<0.001) In multiple regression analysis, the L-spine BMD and PWV had an independent relationship in women after adjusting for age, metabolic syndrome, BMI, smoking, drinking and exercise. (r=-0.229, P=0.015). No independent association was found between PWV and BMD in men. CONCLUSIONS: The association between arterial stiffness and BMD was confirmed in women. However, this association was not statistically significant for men.
Subject(s)
Female , Humans , Male , Absorptiometry, Photon , Body Mass Index , Bone Density , Cholesterol , Drinking , Education , Femur , Health Promotion , Mass Screening , Osteoporosis , Pulse Wave Analysis , Retrospective Studies , Smoke , Smoking , Spine , Vascular Stiffness , Waist CircumferenceABSTRACT
Theoretically, if the odontoblastic layer on the pulpal surface of the dentin is removed, it is impossible to find nucleus after the teeth has undergone endodontic therapy. So dentin does not contain genomic DNA. But it is in existence the possibility that blood permeates into the dentin by endodontic therapy and trauma. Therefore DNA in blood can be detected from the dentin layer. The purpose of this investigation is to evaluate the possibility of individual identification after detection of DNA in the dentin of endodontic created teeth. The authors isolated the human DNA from 40 endodontic treated teeth, performed AmpFLPs by PCR and electrophoresed for detection of STR loci F13A01, LPL gene and X-Y homologous amelogenin gene. The following results were obtained: 1) DNA extraction was possible in 19 endodontic treated teeth out of 40. 2) Sex determination was possible in 12 endodontic treated teeth out of 40 by detection of X-Y homologous amelogenin gene 3) F13A01 locus was detected in 6 endodontic treated teeth out of 40, observed 4 alleles(3.2, 4, 5, 6) and 5 genotypes(3.2-3.2, 3.2-4, 3.2-5, 4-5, 6-6). 4) LPL locus was detected in 7 endodontic treated teeth out of 40, observed 3 alleles(10, 11, 12) and 3 genotypes(10-10, 10-12, 11-12). From the above results DNA extraction, sex determination, amplification of STR locus F13A01, and LPL gene were possible in the endodontic treated teeth and it was suggested that endodontic treated teeth were useful and applicatable as molecular biological samples for individual identification.