ABSTRACT
Background: Mycoplasma pneumoniae, an atypical pathogen, is increasingly recognized as a common and important human pathogen that is implicated in a wide spectrum of clinical diseases involving multiple body systems. The most common presentation is with respiratory illness. An accurate estimate of the extent of Mycoplasma pneumoniae infection in the general population is lacking, and infection is probably more extensive than currently accepted due to difficulties in culturing the organism from clinical samples and unreliable diagnostic laboratory tests. This study was undertaken to evaluate PCR, IgM ELISA, and direct antigen enzyme immunoassay [EIA] as rapid methods for diagnosis of M. pneumoniae infection
Methods: We prospectively analyzed 21 children [12 males and 9 females; age, 1.5-18 years] admitted to MUPH with atypical pneumonia. They underwent clinical, radiological and laboratory examinations. Mycoplasma pneumoniae infection was diagnosed by PCR, IgM ELISA, and direct antigen EIA
Results: Among studied 21 cases, M. pneumoniae infection was diagnosed in 4/21 [19.05%] cases. Among 4 cases positive for M. pneumoniae, IgM was positive in 3 cases [75%], Antigen detection was positive in 3 cases [75%], and PCR was positive in 4 cases [100%]
Conclusion: Our data suggests an association between acute Mycoplasma pneumoniae infection and atypical pneumonia in children. Absence of Coryza and presence of fine rales on chest auscultation in addition to the presence of thrombocytosis implicate M. pneumoniae infection
ABSTRACT
Aim: laparoscopic cholecystectomy gives rise to a very complicated issue concerning the use of antimicrobial prophylaxis. The role of antibiotic prophylaxis in elective laparoscopic cholecystectomy and the relation between post-operative septic complication and bile microbiology will be assessed in this study
Patients and methods: 64 patients were included in the study and divided into two groups; group A: [29 patients] received antibiotic prophylaxis [cefotaxime] and group B [35 patients] received 100m1 isotonic saline [placebo]. Bile sampling and culture was taken for all cases and the patients' were followed up for incidence of sepsis where the wounds were swapped and cultured
Results: bile sampling and culture revealed no growth in 41 cases [64.1%] [19 in-group A and 22 in-group B]. Postoperative infection developed in two cases of group A and in three cases of group, B. Incidence of wound infection was significantly related to incidence of GB perforation [p < 0.0001]. Swabbing of infected wounds and collections revealed infecting organisms other than that detected in bile culture except in one case of group B
Conclusion: there is no relation between bile microbiology and postoperative infection, so, antibiotic prophylaxis is to be used in high-risk patients and in patients suspected to have difficult operation and GB perforation
ABSTRACT
The aim of this study was to evaluate serum tumor markers [CA15-3, CEA and TNF] and ER receptors in serum and urine. It was done on 20 patients with breast cancer, 20 patients with benign breast lesions and 20 normal controls. A significant high level of the mean serum value of CA15-3 in cancer patients compared with benign and control groups. The mean preoperative levels returned nearly to normal in completely cured cases, while it was in a high level if there was metastasis or recurrence. In conclusion, serum CA15-3 and CEA levels could be valuable as a prognostic parameter and may have a role in monitoring patients with breast cancer. Other tested tumor markers, especially TNF may be complementary in this regard, while receptor patterns were important for determining the modality and response of treatment
Subject(s)
Biomarkers, Tumor , Tumor Necrosis Factors , Receptors, Estrogen , Carcinoembryonic Antigen , Receptors, ProgesteroneABSTRACT
Comparing the characters of the mutants and their parent strains, the isolated mutants were found to be different in some morphological and colonial characters, haemolysin production, haemagglutination activity, biochemical reactions and antibiotic sensitivities