Application of Polymerase chain reaction technique and Immuno-chromatography assay to detect antibody against M. tuberculosis in pleural fluid patients

At the Hospital No 103, PCR technique was used for detecting M. Tuberculosis immediately in pleural fluid of 39 patients with tuberculosis pleuresia and 10 patients with primary cancer pleuresia. By PCR reaction tuberculosis bacterial DNA was detected successfully in pleural fluid of 92,3% of paitents. The technique had got 63,8% of sensitivity and 100% of speccificity. By immunochromatography, IgG antibacterial antibody in pleural fluid was detected in 89,7% of patients. The combination of these 2 methods were necessary in giving high efficacy in definite diagnosis. The technique of PCR could be applied in various institution for earlier diagnosis with higher accuracy.

The correlation of HBV-DNA with liver enzyme indicators and other markers of hepatitis B virus in chronic aclive hepatitis B patients

Research on 31 patients (include 24 man and 7 woman) at 20 to 60 years old who treated active hepatitis B at The army centre hospital 105 from 1/1999 to 6/2002. Result: HBV-DNA level in plasma increase follow agreeable rate with SGPT content: SGPT increase to > 100U/L correlative HBV-DNA patients(+) ≥10copies, 16,13% HBV-DNA patients (-) from 5 to 10 copies. The development of the disease in active hepatitis B patients was showed at relationship of marks of HBV with HBV -DNA(+) 70,96%, Anti-HBV-IgG (+) 100%, anti-HBc0IIgG (+) and anti-HBc-IgM (+) 12,90%, HBV-DNA (+) and HBeAg (+) 41,94%, HBV-DNA(+) and anti-HBe (-) 38,72%. HBV-DNA may be importance mark in following up development of the disease

The economic burden of unintentional injuries

This study aims to estimate the costs of various unintentional injuries in Ba Vi district during one year, and to describe how the economic burden of unintentional injuries was distributed between households, government and the health insurance agency. Our cohort study involved four cross sectional household surveys among sampled communities in Ba Vi district during the year 2000, each asking about injuries in the preceding 3 months. The costing system in public health care in Viet Nam was applied as well as information from the victims. The total cost of injuries for the Ba Vi district was estimated to be 3.412.539.000 VND. Equivalent to the yearly income of 1800 people. 90% of this economic burden fell on households, only 8% on government and 2% on the health insurance agency. The cost of a severe injury to the victim corresponded to approximately 7 months of earned income. Home and traffic injuries together accounted for more than 80% of the total cost, 45% and 38% respectively.