ABSTRACT
The cytotoxicity of the biomaterials is a key issue that should be addressed prior to pre-clinical applications. This study was designed to evaluate and compare the cytotoxixity of two forms of bioactive glasses:nanopowder: and micropowder. Human HGF1-PI53 gingival fibroblast cells were used to evaluate the cytotoxicity of 0.5, 1, 1.5, 2, 5, 10, 15 and 20 mg/mL concentrations of the two bioactive glasses via MTT assay. The results were statistically analyzed using analysis of variance and Tukey's test. A p-value less than 0.05 was considered statistically significant. Results showed that two bioactive glasses hadstatistically significant differences at 5, 10, 15 and 20 mg/mL concentrations [p-value < 0.05] and there was no correlation between time and cell cytotoxicity of bioactive glasses [p-value > 0.05], using t-test and Spearman's correlation coefficient. We conclude that that cytotoxicity of nanopowder bioactive glass at concentrations /= 5 mg/mL in the first 48 h of applications