Experimental study of the effects of time-phased cryopreservation and resuscitation on biological characters of osteoblasts / 生物医学工程学杂志

This is an in vitro study to explore the effects of cryopreservation and resuscitation on the biological characteristics of osteoblasts at different times. Osteoblasts taken from the crania of newly born SD rats were cultured. Comparative studies were made on the cells' proliferation, the activity of alkaline phosphatase (ALP), and the number of live cells among fresh cultured osteoblasts and cells after the inception of cryopreservation and resuscitation at time-periods of one, three, six months respectively. The results showed that there were no significant differences among four groups in cell proliferation and in activity of ALP (P > 0.05). Yet, after cryopreservation and resuscitation, there were significant differences between the six-month group and the other three groups (P < 0.05). The results also showed, after cryopreservation and resuscitation, there were no significant differences between the control group and the one-month and three-month groups, respectively (P > 0.05). These findings indicated that the live cells might decrease in number after the osteoblasts were cryopreserved for too long a period, but after cryopreservation and resuscitation, the cells still retained the original biological characteristics of osteoblasts.

Expression of vascular endothelial growth factor and transforming growth factor-beta 1 in synovial membrane of osteoarthritis and their relationships / 中国组织工程研究

BACKGROUND: The vascular endothelial growth factor (VEGF) and transforming growth factor-?1 (TGF-?1) are the family members of the synthesis and decomposition class cell factors, which closely relate to osteoarthritis. But the expression of VEGF and TGF-?1 in previous reports mainly focuses on the cartilage cell and matrix aspect in osteoarthritis is superficial, especially in synovial membrane tissues. OBJECTIVE: To further understand the expression of VEGF and TGF-?1 in the synovial membrane of osteoarthritis, and to study the relationship between the two factors. DESIGN, TIME AND SETTING: The in vitro cytology experiment was completed in the Molecular Biology Laboratory of Zibo Central Hospital, Affiliated Hospital of Binzhou Medical College from January 2003 to September 2004. PARTICIPANTS: A total of 30 pathology specimen of synovial membrane which conformed to the diagnoses standard of osteoarthritis, and 8 pathology specimen of synovial membrane which were unnatural death or traumatic amputation healthy people were selected. METHODS: All synovial membrane specimen were performed immunohistochemistry staining, peroxidase solution, normal horse serum, first antibody, polyvalent biotinylated antibody, streptavidin-peroxydase solution, and DAB solution were added orderly, then followed by haematoxylin staining. In the control group, all procedure was the same as the synovial membrane instead of the first antibody was replaced with PBS. MAIN OUTCOME MEASURES: The expression of VEGF and TGF-?1 in the synovial membrane and their relationship. RESULTS: The positive expression rates of VEGF and TGF-?1 were 86.7% and 13.3%, respectively. Compared with normal tissues, the positive expression rate of TGF-?1 was lower (P