"Treatment of class Ⅲ malocclusion by ""surgery-first approach""" / 中华医学美学美容杂志

Objective To investigate the the treatment of class Ⅲ malocclusion by orthognathic surgery combined with postoperative orthodontics.Methods Nine patients with skeletal class Ⅲ malocclusion were treated by surgery-first approach without pre-surgical orthodontic from January 2012 to August 2014.The studied sample consisted of 7 women and 2 men (aged 15-28 years old, mean age 19.7 years), who had obvious mandibular protrusion.2 to 3 days after surgery, intermaxillary traction was used to made the maxilla and mandible together by board;we replaced a rubber band every 2 to 3 days and lasted for four weeks.We would dismantle board and performed conventional orthodontic treatment after patient's facial swelling subsided, and the positional relationship between the jaw stabilized.Results The face type of 9 patients were greatly improved after orthodontic treatment for 6.5 to 19.5 months.Patients and their family members felt satisfied, and their occlusal function returned to normal.At 3 to 32 months follow-up, the postoperative appearance and occlusion were becoming good without obvious signs of recurrence.Conclusions The surgery-first approach is an effective method to treat skeletal class Ⅲ malocclusion.

Bulk culture of Helicobacter pylori-specific CD4+T cells in vitro for identification of Th epitopes / 中华微生物学和免疫学杂志

Objective To screen an optimum method for in vitro culture of Helicobacter pylori-spe-cific CD4+T cells and apply it to immunodominant Th epitopes screening .Methods PBMCs were isolated from subjects positive for Helicobacter pylori infection and were stimulated with HpaA recombinant protein . Various induction conditions including serum containing mediums , concentrations of antigen and time were screened to obtain an optimum method for in vitro culture of Helicobacter pylori-specific CD4+T cells.The cells were harvested and stimulated using HpaA synthesized overlapping peptide pool .The percentage of an-tigen-specific CD4+T cells was evaluated by intercellular cytokine staining of interferon-γand the results were compared under different conditions .The possible immunodominant Th epitopes were screened by using synthetic overlapping peptides .Results Antigen-specific CD4+T cells were well cultured in RPMI 1640 culture medium containing human AB serum in comparison with those cultured in fetal bovine serum based medium.The highest percentage of antigen-specific CD4+T cells was achieved when stimulated with HpaA recombinant protein at the concentration of 0.2 μmol/L.CD4+T cells in response to the stimulation of 0.2μmol/L of HpaA recombinant protein was observed on the ninth day after culture and its peak was reached on the fifteenth day .A possible immunodominant Th epitope ( HpaA220-237 ) was screened in subjects with He-licobacter pylori-infection by using synthetic overlapping peptides .Conclusion Helicobacter pylori-specific CD4+T cells were successfully cultured in vitro by using RPMI 1640 culture medium containing human AB serum and stimulated with 0.2 μmol/L of HpaA recombinant protein for fifteen consecutive days .This cul-ture method could be applied to immunodominant Th epitopes screening and provide evidences for further in -vestigation on the development of Helicobacter pylori epitope-based vaccine .

Constructive tissue engineering bone with BMSC sheets and PLGA scaffold / 中华医学美学美容杂志

Objective To construct bone marrow stem cell sheets and to investigate its effects in the process of osteogenesis.Methods BMSCs were differentiated into osteoblasts and then seeded into a temperature responsive culture dish to construct BMSC sheets.PLGA scaffolds in which both BMSC suspension and BMSC sheets were added,were implanted into the left side of the dogs' mandible.In the other side,PLGA scaffolds that were not wapped with BMSC sheets were implanted as control.At 16 weeks,the samples were processed for radiological analysis and histological examination.Results Cells in the BMSC sheets grew well.In the experimental side,the optical density of the samples was higher than that of the control side (P＜0.05) and plenty of lamellar bones and Haversian system were observed.Conclusions The formation of lamellar bones can be promoted by PLGA scaffolds and BMSC sheets in the process of tissue engineering bone reconstrution.

Relationship between single nucleotide polymorphism of urokinase-type plasminogen activator gene and tongue carcinoma / 肿瘤研究与临床

Objective To determine association between tongue carcinoma and polymorphism of urokinase-type plasminogen activator (PLAU) gene.Methods PLAU genotypes of 97 patients with tongue carcinoma and 91 health controls were examined by the PCR-RFLP method.Statistical analyses included a chi-square test for homogeneity and logistic regression analysis.Results The polymorphism in PLAU gene was rs2227564 C/T.Logistic analyses indicated that compared with CT and TT genotypes,CC genotype was risk factor for development of tongue carcinoma (adjusted OR =1.281,95 ％ CI 1.098-2.577,P =0.037).Conclusion PLAU polymorphism may be associated with development of tongue carcinoma.

A study of repairing mandibular defect using tissue engineering bone with bone marrow stem cells cell sheets in dog / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To reconstruct mandibular defect using tissue engineering bone with bone marrow stem cells (BMSCs) cell sheets and investigate the effect of cell sheets on osteogenesis.</p><p><b>METHODS</b>BMSCs were isolated with the method of density gradient centrifugation from canine and cultured. BMSCs were induced to differentiate to osteoblasts. BMSCs induced were fabricated to BMSCs cell sheets. The poly (lactic-co-glycolic acid) (PLGA) wrapped with cell sheets were implanted into the mandibular defect in the left side (experimental side). PLGA wrapped without cell sheets were implanted into the right side (control side) of mandibles. 16 dogs were evenly divided into 4 groups, and one group of them was executed in 4, 8, 12, 16 weeks for gross investigation and histological observation.</p><p><b>RESULTS</b>The osteogenesis of experimental side was better than that of control side. 16 weeks after implantation, most areas of the mandibular defect were replaced by fresh bone tissue. Compact bone similar to normal bone tissue formed in the lingual defect of mandible and had bony union with the bone stump. The optical density of the fresh bone in the experimental side was higher than that of the control side, there was a significant difference between the two methods (P<0.05). Plenty of lamellar bones formed in experimental side and Haversian system, as well as red marrow, were observed.</p><p><b>CONCLUSION</b>Tissue engineering bone with the structure of lamellar bones can be formed by the technology of BMSCs cell sheets.</p>

Anatomic structure of the mandibular canal / 中国组织工程研究

BACKGROUND:n mandibular posterior dental implantation,injury to the inferior alveolar nerve sometimes occurs because of mandibular canal going across mandibular body.This restricts the use of dental implantation at this site.Therefore,it is essential to understand the anatomic structure of inferior alveolar nerve canal in mandibular posterior dental implantation.OBJECTIVE:To observe the intramandibular course of and anatomic structure of inferior alveolar nerve canal.METHODS:Fifteen adult complete mandible specimens with teeth and 4 fresh mandible arterial infusion specimens were researched.All the specimens had complete dentition and there were no obvious absorption in alveolar bone.The course of inferior alveolar nerve canal and its dimension including transverse and longitudinal diameters of mandibular canal and the distance between mandibular canal and mandible each side (superior,inferior,buccal and lingual side) were measured in 15 adult mandibles with teeth.The relationship between blood vessels and nerve of the canal was observed in 4 fresh arterial infusion specimens.RESULTS AND CONCLUSION:The distance between the medial border of the mandibular canal and the lingual wall was shorter than that of the lateral wall of the mandibular canal to the buccal wall (P < 0.01);The length from the upper wall of mandibiular canal to the top of the alveolar ridge was longer than that of the inferior border of the mandibular canal to the inferior border of the mandible (P < 0.01).The longitudinal diameter was smaller than the transverse diameter (P < 0.05),namely,the cross section of the mandibular canal was an ellipse with a longer longitudinal diameter.There was no significant difference between the transverse and longitudinal diameters of the canal in the anterior and posterior teeth region of the mandible.The inferior alveolar nerve and its associated blood vessels were located within a nervous vascular bunch in the mandibular canals.In every fresh specimen the blood vessels lay above the nerve.There were small branches of blood vessels surrounding thenerve.The mandibular canal ran towards the lingual side and was close to the inferior margin of the mandible.

An animal experiment of construction of functional tissue-engineered bone with cell sheet technology / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To construct functional tissue-engineered bone with cell sheet technology and method of traditional bone tissue engineering.</p><p><b>METHODS</b>Canine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 degrees C, 5% CO2 and saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 degrees C. Demineralized bone matrix (DBM) and platelet-rich plasma (PRP) were prepared, and complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. Complex of DBM/PRP/BMSCs was implanted under the right latissimus dorsi muscle.</p><p><b>RESULTS</b>When temperature dropped at 20 degrees C, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/ BMSCs group.</p><p><b>CONCLUSION</b>Cell sheet technology combined with traditional bone tissue provides a new way for construction of ideal functional tissue-engineered bone.</p>

Complex of dog allogenic decalcified bone matrix and bone marrow stromal cell sheets In vitro culture and observation / 中国组织工程研究

BACKGROUND: How to reconstruct tissue-engineered bone with structure similar to natural bone iS a problem in the development of tissue engineering. Cell sheet engineering technology enables novel approaches to construction of tissue-engineered bone. OBJECTIVE: To observe the biocompatibility of call sheets to decalcified bone matrix (DBM) and their growth on DBM. DESIGN, TIME AND SETTING: An in vitro observation was performed at the Central Laboratory, Affiliated Hospital, Qingdao University Medical College between June and September 2009.MATERIALS: Dog bone marrow stromal cell sheets were prepared using temperatura-responsive medium. Dog DBM was prepared by defatting, decalcification, and noncotlagen protein removal procedures. METHODS: DBM surface was covered by call sheets prepared by temperature-responsive technology and cultured with DMEM containing 10% fetal bovine serum and osteoinductive agent.MAIN OUTCOME MEASURES: Under scanning electron microscope, DBM structure, as well as the attachment and growth of cell sheets on DBM surface, was observed. Porosity and aperture size of DBM were calculated. RESULTS: DBM exhibited a three-dimensional latticed structure, with a porosity of approximately 75%. The mean aperture size was (250.11±98.89) μm, exhibiting a normal distribution. Cell sheets well attached to and grew on DBM surface, and rapidly proliferated.CONCLUSION: Cell sheets show good biocompatibility to DBM. DBM/cell sheets complex can be applied in tissue-engineered bones, which promotes the construction of tissue-engineered bone with structure similar to natural bone.

Culture and preparation of dog bone marrow mesenchymal stem cell sheet in vitro / 中国组织工程研究

BACKGROUND:There are some disadvantages in harvesting and transferring cells in the traditional tissue engineering technique,and it is difficult to form dense tissues,which significantly limits the development of tissue engineering.OBJECTIVE:To explore the culture and fabrication of dog bone marrow mesenchymal stem cell(BMSC)sheet in vitro.METHODS:Bone marrow was extracted from dogs following anesthesia.BMSCs were isolated with the method of density gradient centrifugation in vitro.BMSCs at passage 4 at a density of 1×10~9/L were incubated in the temperature-responsive culture dishes with a diameter of 3.5 cm,and cultured in an incubator at 37 ℃,5% CO_2 and saturated humidity.The temperature of the incubator was changed from to 37 V to 20 ℃ to prepare BMSCs cell sheet for 20 minutes.Cell morphological changes and cell sheet formation were observed under an inverted microscope.RESULTS AND CONCLUSION:Dog BMSCs following 24 hours of primary culture presented ellipse or polygonal shape.Most cells adhered at hour 72,and cell colonies were visible at day 7.Cells showed long spindle and completely confluence at day 12,with unclear boundary.BMSCs in the temperature-responsive culture dishes presented short spindle shape,and gradually separated from the dish bottom,forming entire cell sheet containing extracellular matrix at 20 V.These verified that dog BMSCs can be effectively obtained with method of density gradient centrifugation.Complete cell sheet layer can be fabricated with temperature-responsive culture dishes.

Preparation of canine bone marrow stromal stem cell sheets and investigation on their osteoblastic differentiation / 中国组织工程研究

BACKGROUND:Conventional methods,including trypsin digestion and cells transfer using single call suspension,have many drawbacks,which limit the development of bone tissue engineering.OBJECTIVE:To culture bone marrow stromal stem calls,induce osteoblastic differentiation,and prepare cell sheets.METHODS:Canine bone marrow stromal calls were isolated by density gradient centrifugation technique,inoculated into DMEM medium,and induced to differentiate into osteoblasts.Complete call sheets were harvested by call sheet engineering based on the temperature change of temperature-responsive medium.RESULTS AND CONCLUSION:Immediately after inoculation,primary calls were scattered on the bottom of culture flask,presenting a transparent spherical body with a good refractive capacity.At 12 hours,calls exhibited a long shuttle shape,reached complete confluency,and grew in a whirlpool-like fashion.After osteoblastic induction,the majority of bone marrow stromal stem calls appeared tetragonal,polygonal,and squamose.At 21-28 days,round or oval-shaped calcified nodules formed.When the bone marrow stromal stem calls in the temperature-responsive culture dishes were cooled below the critical temperature 32℃,cells were gradually detached from the bottom of culture flask and formed complete bone marrow stromal stem call sheets.These findings indicate that density gradient cantrifugation technique can be used to successfully isolate and culture canine bone marrow stromal stem cells to differentiate into osteoblasts and call sheet engineering enables to harvest complete bone marrow stromal stem call sheets.

Clinical analysis of oral and maxillofacial spaces infections in diabetic patients:a report of 31 cases / 中华全科医师杂志

The characterization and management of oral and maxillofacial spaces infections in diabetic patients were studied in order to determine the pattern of this clinical condition and formulate a management plan.There were 31 cases with average age of 61 years(s=9);the mean hospitalization time was 14 days(s=6);the average fasting blood glucose level on admission was 10.4 mmol/L.Of the 31 patients 20 were multiple-space infections and 11 were single-space infections.13 patients had major complications during admission.Odontogenic infection was the most common cause of the space infections.Streptococcus viridians and staphylococcus aureus were common organisms(5/19,4/19)identified through pus and/or blood cultures.Early surgical incision and drainage,perfect blood glucose control,intravenous antimicrobial therapy,preventing asphyxia and managing major complications are necessary and effective approaches for the management plan.

Platelet-rich plasma and latissimus dorsi fascia with blood vessels in the vascularization of tissue-engineered bone: A histological & radiological observation / 中国组织工程研究

BACKGROUND: It is still disputed whether several growth factors of platelet-rich plasma and the fascia with blood vessels can promote bone regeneration and vascularization. OBJECTIVE: To evaluate the effects of platclet-rich plasma and the fascia with blood vessels on the vascularization of tissue-engineered bone composited by marrow stromal stem cells (MSCs) and decalcified bone matrix (DBM). DESIGN, TIME AND SETTING: The present single-sample observation, self-control animal experiment was performed at the Central Laboratory Center, Affiliated Hospital of Qingdao University Medical College between October 2004 and November 2007. MATERIALS: Twelve healthy hybrid dogs, aged 11-12 months, weighing 20-25 kg, equal number of males and females, were included for this study. METHODS: ①MSCs were isolated from dog bone marrow by density gradient centrifugation, followed by in vitro adherent culture and ontogenesis culture.Dog femur was taken for preparation of DBM and composited with MSCs.②The back of each dog was divided into 4 regions (A,B,C,and D).In the regions A and B, DBM/MSCs/platelet-rich plasma composites were transplanted. In the regions C and D, DBM/MSCs composites were transplanted. The implants for the regions A and C were wrapped with latissimus dorsi fascia with blood vessels. The implants for the regions B and D were wrapped with blood vessels-free superficial fascia from back. At weeks 4,8,and 12,4 dogs comprising 2 males and 2 females, were sacrificed following anesthesia for specimen harvesting. MAIN OUTCOME MEASURES:①MSC morphology was observed utilizing a phase contrast microscope, and cell growth curves were portrayed by methyl thiazolyl tetrazolium colorimetric assay.②Morphology of osteoblasts induced was observed by modified alkaline phospharase (ALP) staining (Ca-Co method), Von Kossa method, alizarin red method and calcium node staining.③Composite structure was observed through the use of inverted phase contrast microscope and scanning electron microscope.④Gross, radiological, and histological observations. RESULTS: ①Both induced osteoblasts and MSCs exhibited ALP and calcium node stainings positive.②The growth curves of induced osteoblasts and MSCs presented with typical "S" shape.③Following composite culture of allogenic DBM and MSCs, MSCs grew well, rapidly proliferated and could secrete a great amount of extraccllular matrix.④At each time point, osteogenic bone mass, bone absorbance, and vascularization were better in regions A,B,and C than in region D (P＜0.05). CONCLUSION: Histological and radiological observations have confirmed that both platelet-rich plasma and the fascia with blood vessels can synergistically promote the formation and vascularization of tissue-engineered bone.

In vitro osteoblastic differentiation and identification of rat bone marrow mesenchymal stem cells by whole bone marrow adherent culture / 中国组织工程研究

BACKGROUND: Many operations for isolating, purifying and identifying bone marrow mesenchymal stem calls (BMSCs) are complicated and cost much. Also they have great effect on cell activity. Whether whole bone marrow adherent culture can avoid above-mentioned disadvantages remains unclear. At present, many studies huve been done to confirm an effective and low cost method for isolating, purifying and identifying such cells.OBJECTIVE: This study is to in vitro induce and differentiate rat BMSCs by whole bone marrow adherent culture,and to identify the cells.DESIGN: A controlled observational experiment.SETTING: Qingdao University Medical College.MATERIALS: This study was carried out in the Laboratory of Oral Cavity and Laboratory of Molecular Biology (provincial level) Qingdao University Medical College between November 2005 and March 2007. Twenty Wistar rats of either gender, aged 3 to 4 weeks, of SPF grade, weighing 120-150 g, were provided by the Qingdao Laboratory Center. The protocol was carried out in accordance with animal ethics guidelines for the use and care of animals. Fetal bovine serum (FBS, Hangzhou Sijiqing Bioengineering Material Research Institute), alkaline phosphatase (ALP) kit (Nanjing Jiancheng Bioengineering Research Institute), reverse transcription kit (American Promega Corporation) and primer (Shanghai Bioengineering Co.,Ltd.) were used in this study.METHODS: Adult rat BMSCs were isolated and cultured by whole bone marrow adherent culture. They were digested with 2.5 g/L trypse and inoculated at a density of 5 ×107 L-1 in 6-well culture plate. Then, the cells were divided into experimental group and control group. Inducing culture medium was added to experimental group, and the same amount of basic culture medium was added to control group. ① Cell differentiation and calcium tuberculation were observed under the inverted microscope. ② Biological characteristics of induced cells were detected by calcium tubercle Von Kossa and alizarin Bordeaux. ③ALP activity was detected by diazo salt staining. ④Human core binding factor alpha subunit-1 (Cbf α-1), osteocalcin (OCN) and osteoblast-specific Osterix (OSX) mRNA expressions were detected by reverse transcription-polymerase chain reaction (RT-PCR).MAIN OUTCOME MEASURES: ① Induction and differentiation results of cells. ② Biological characteristics of cells induced by rat BMSCs. ③ ALP activity. ④ Cbf α-1, OCN and OSX expressions.RESULTS: ①Inducing culture medium was added in the serial subcultivation. About 9 days later, cell clones were connected to each other. On about 21 to 28 days, some pykno-round mineralized tubercles appeared. Meanwhile,control cells were connected to each other, but they did not form the tubercle. ② In the experimental group, when MSCs were induced for 21 to 28 days, obvious round or oval calcified tubercles were seen by naked eyes. The results of Von Kossa staining exhibited black sediments, and those of alizarin Bordeaux staining exhibited salmon tubercles. Calcium tubercles were not found in the control group. ③The ALP activity after 2 weeks of induction was obviously increased in the experimental group, but was relatively weak in the control group. ④In the experimental group,Cbf α-1, OCN and OSX expressions were significantly increased after induction.CONCLUSION: After being in vitro induced and differentiated by whole bone marrow adherent culture, rat BMSCs exhibited morphological and biological characteristics similar to typical osteoblasts.

Trigeminal neuralgia and jaw bone cavities: analysis of 45 cases / 中国组织工程研究

BACKGROUND: Odontogenic infection factor has been given much importance in the study of the etiology of secondary trigeminal neuralgia,and the theory of jaw bone cavities is proposed. OBJECTIVE: To study the relationship of the jaw bone cavities and the etiology of trigeminal neuralgia.DESIGN: A self-controlled trial.SETTING: Department of oral and maxillofacial surgery of a university hospital.PARTICIPANTS: The patients with the trigeminal neuralgia were treated in Department of Oral and Maxillofacial Surgery, Affiliated Hospital of the Medical College of Qingdao University from February 1994 to December 2003, from whom 45 were selected for this study, including 15 males and 30 females with altogether 74 jaw bone cavities.METHODS: Curettage of the jaw bone cavities was performed in these cases, and visual analogue scale(VAS) was adopted for evaluation of the postoperative pain.MAIN OUTCOME MEASURES: ① VAS; ② Pathological examination and bacteria culture of the specimens.RESULTS: Pain relief was achieved in 33 cases(73.3% ) after the first surgery and in 10 cases(22.2% ) after a second or third surgery. In 2 cases (4.5%), the pain was alleviated but medication was still needed for pain control. Pathological examinations in most cases identified predominantly in flammatory and granulation tissues.CONCLUSION: Jaw bone cavities may be one of the major etiologic factors of trigeminal neuragia.

The analysis of formant characteristics of vowels in the speech of patient with cleft palate / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the formant frequency of vowels in the sequence therapy of patient with cleft palate.</p><p><b>METHODS</b>The formant frequency of vowels [a], [e], [i], [u] of normal children and postoperative patients with and without speech therapy was measured and analyzed by VS-99.</p><p><b>RESULTS</b>1. The mean value of F1, F2, F3 of [a] did not show significant difference among the three groups (P > 0.05). 2. The difference of mean value of [e] was significant between control group and pre-speech-therapy group, and between pre-speech-therapy and post-speech-therapy group (P < 0.05), but no significant difference was found between post-speech-therapy and control group(P > 0.05). The mean value of the formant in post-speech-therapy was higher than that of pre-speech-therapy. 3. The difference of mean value of [i] was significant between pre-speech-therapy and post-speech-therapy (P < 0.05), the mean value of F2, F3 in post-speech-therapy group decreased significantly compared with control (P < 0.05). 4. The difference of mean value of [u] showed significance between pre-speech-therapy and post-speech-therapy (P < 0.05), while the differences among other groups were insignificant (P > 0.05).</p><p><b>CONCLUSION</b>Surgical repair of cleft palate cannot make all patients obtain perfect Velopharyngeal competence (VPC), while speech therapy can improve patient's pronunciation. Speech spectrum analysis can judge the effect of cleft palate therapy objectively.</p>

Application of obturator to treat velopharyngeal incompetence / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To evaluate the effect of a system of velopharyngeal incompetence (VPI) management after the application of obturator.</p><p><b>METHODS</b>Using nasopharyngofiberoscope (NPF) and a computer analysis system, we quantitatively analyzed the improved state of velopharyngeal incompetence in 100 patients with unilateral and/or bilateral cleft palate.</p><p><b>RESULTS</b>The velopharyngeal closure (VPC) can be greatly improved by using a temporary oral prosthesis (obturator) and speech training. An objective quantitative standard was established to evaluate the change of velopharyngeal closure of cleft palate patients after surgery and conservative treatment.</p><p><b>CONCLUSIONS</b>The method used is more succinct, accurate and practical than previous methods. In order to reflect the state of velopharyngeal incompetence, the concept of improvement rate of velopharyngeal incompetence (IRVPI) is put forward.</p>

A study of rebuild of a three-dimensional anatomic model of mandibles / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to rebuild the anatomic structures of the mandibular bone and the canal, and to testify the reliability of the rebuilt model.</p><p><b>METHODS</b>15 mandibular bones with teeth are chosen, and a three-dimensional model was built with the method of CT. The slices vertical to the compensating curve were made in every dental position. Then the authors collected the data of alveoli and mandibular canal in every slice. The same work was done to the bone specimen, and then comparative analysis was done.</p><p><b>RESULTS</b>The wideness of the alveoli and 10 mm bellow increased from mesial to distal position. It was the same of the alveoli from the top to the bottom. The mandibular canal lied in the inner and inferior side of the mandibular body. There was no significant difference in data collected with these two methods.</p><p><b>CONCLUSION</b>Different types of dental implants should be chosen according to the anatomic characteristic of the mandibular bone in every dental position before the operation to avoid complications. And a reliable, accurate and direct method of planning an implant operation is to rebuild a three-dimensional model.</p>

Repair of mandibular defect with tissue engineered bone / 实用口腔医学杂志

Objective: To study the effects of tissue engineered bone in the repair of mandibular defect.Methods:The experiments were conducted in 12 dogs.Bone marrow stromal stem cells(BMSCs) of dog were cultured in DMEM containing 100 ml/L fetal bovine serum and induced to differentiate towards osteoblasts.Then the cells were seeded onto absorbable polylactic acid(PLA) compounded with rhBMP-2(0.16 mg for each implant), the composite was implanted into the oval-shaped mandibular bone defect in the size of 30 mm?12 mm on one side,another side was used as blank control.The dogs were divided into 4 grups with 3 in each group.PLA/rhBMP/BMSCs,PLA/rhBMP,PLA/BMSCs and PLA were used as the implants in group A,B,C and D respectively. 2, 4 and 8 weeks after implantation,the effectiveness of bone formation was evaluated by means of gross observation,histological and scanning electronic microscope ( SEM) examination. Results: In group A new bone formation in the implanted defects was observed 4 weeks after operation, the defects were replaced by muture bone tissue 8 weeks after operation. In group B a little new bone was found in the implanted area 4 weeks after operation and fibrous bone was observed 8 weeks after operation. In group C chondral ossification was found and in group D fibrous tissue was found in the bone defects 8 weeks after operation.Conclusion:PLA/rhBMP/BMSCs may be feasible in the repair of bone defect.

Analysis of the salivary glucose level in the patients with diabetes mellitus / 实用口腔医学杂志

Objective: To analyse the level of glucose concentration in saliva and to study the relationship between the level of blood glucose and that of salivary glucose.Methods: Blood glucose level and the glucose concentration in unstimulated mixed saliva taken from testee were measured with Beckman SYNCHRON CX7 System in 60 patients with diabetes mellitus and 60 healthy subjects. Results: The average value of salivary glucose concentration in experimental group was (1.950?0.179) mmol/L, that in control group was (0.953?0.124) mmol/L(P

ELECTROMYOGRAPHIC STUDY OF THE LATERAL PTERYGOID MUSCLE / 解剖学报

The electromyography of the superior and inferior heads of lateral pterygoid muscle of 15 human subjects were recorded. It has been observed that marked active potential occurred in the superior head of the ipsilateral pterygoid muscle and the inferior head of the contralateral muscle during a one-side molar bite. Marked active Potential appeared in the superior head of the lateral pterygoid muscle during the closing movement of the jaw, whereas it appeared in the inferior head during the opening movement.It has not been confirmed that the lateral pterygoid muscle is an important protractor of the mandible, as only relatively weak active potential was recorded in the two heads of the muscles on both sides during the protraction of the jaw. Very marked active potential has been observed in the ipsilateral prenygoid muscle during the lateral movement of the mandible, while in the contralatenal superior head it occurred only slightly. The. above finding does not correspond to the descriptions found in current text-books.The two heads of the lateral pterygoid can therefonose considered as two functionally and structurally distinct muscles.