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1.
Arq. bras. med. vet. zootec ; 61(supl.1): 126-134, nov. 2009. ilus, tab
Article in Portuguese | LILACS | ID: lil-536309

ABSTRACT

Realizou-se um estudo para caracterizar a situação epidemiológica da brucelose bovina no Estado do Tocantins, entre fevereiro de 2002 e agosto de 2003. O Estado foi dividido em seis áreas com características produtivas homogêneas (circuitos produtores). Para cada área, foi calculada uma amostragem simples aleatória de 300 propriedades, com o objetivo de estimar a prevalência de focos de brucelose além da prevalência de fêmeas bovinas adultas soropositivas. Para isso, foram amostradas de 10 a 15 vacas com idade superior a dois anos em cada propriedade. Um total de 20.908 soros foi obtido de 1.842 propriedades. A prevalência de focos de brucelose foi de 21,2 por cento [19,3-23,1 por cento] e a prevalência de fêmeas bovinas adultas soropositivas de 4,4 por cento [3,6-5,3 por cento] para o Estado. Quando se considerou o circuito produtor, observou-se que os circuitos 1, 2, 3 e 5 tiveram prevalência de focos significativamente maior que os circuitos 4 e 6. Os resultados da prevalência nos circuitos 1, 2, 3 e 5 foram de: 16,0 por cento [12,1-20,6 por cento], 37,6 por cento [32,1-43,4 por cento], 26,4 por cento [21,5-31,7 por cento] e 29,3 por cento [24,3-34,7 por cento], respectivamente. Nos circuitos 4 e 6, foram de 5,8 por cento [3,5-9,1 por cento] e 8,6 por cento [5,7-12,2 por cento], respectivamente. Em cada propriedade, foi aplicado um questionário epidemiológico, com o objetivo de avaliar o grau de associação de possíveis fatores de risco com a doença. Os fatores de risco (odds ratio, OR) associados à condição de foco de brucelose foram: rebanho com mais de 120 vacas (OR= 2,0) e abate de reprodutores na propriedade (OR= 1,52). Vacinação contra brucelose (OR= 0,37), presença de piquete de parição (OR= 0,72) e exploração de leite (OR= 0,63) apresentaram-se como fatores de proteção.


A study was carried out to characterize the epidemiological situation of brucellosis in the State of Tocantins from February 2002 to August 2003. The State was divided into six regions with a homogeneous productive system. For each region, a simple random sample was calculated to estimate the prevalence both in farms and cows older than two-year. To achieve this, from 10 to 15 adult cows (older than two-year) were sampled. A total of 20,908 sera from 1,842 farms were obtained. For the whole State of Tocantins, the prevalence of positive farms (or farms with at least one positive animal) was 21.2 percent [19.3-23.1 percent]. When the production regions were considered, the prevalences for the regions 1, 2, 3, and 5 were: 16.0 percent [12.1-20.6 percent], 37.6 percent [32.1-43.4 percent], 26.4 percent [21.5-31.7 percent], and 29.3 percent [24.3-34.7 percent], respectively. In the regions 4 and 6, the prevalences were 5.8 percent [3.5-9.1 percent] and 8.6 percent [5.7-12.2 percent], respectively. In each visited farm, a questionnaire was applied, in order to evaluate the association between with possible risk factors and the brucellosis. The risk factors (odds ratio, OR) associated with the infected herds were number of cows above 120 (OR= 2.0) and slaughtering of breeding animals in the farm (OR= 1.52). Vaccinating against brucellosis (OR= 0.37), presence of birth pen (OR= 0.72), and dairy farm (OR= 0.63) presented as protective factors.


Subject(s)
Animals , Cattle , Brucella/isolation & purification , Brucellosis, Bovine/epidemiology , Brucellosis, Bovine/immunology , Brucella Vaccine/administration & dosage , Brazil/epidemiology , Communicable Disease Control/methods , Insemination, Artificial/methods , Risk Factors , Rose Bengal
2.
Mem. Inst. Oswaldo Cruz ; 102(5): 639-642, Aug. 2007. tab
Article in English | LILACS | ID: lil-458627

ABSTRACT

Mycobacterium was verified in animals from a Brazilian dairy herd, a total of 42 samples from 30 cows were submitted to culture and the isolated strains were analyzed by two polymerase chain reaction (PCR), the first specific for species belonging to the Mycobacterium complex (MTBC) and the other for differentiating M. tuberculosis from M. bovis. Twenty seven samples (64.3 percent) from 18 animals (60 percent) were positive for mycobacteria by culture, including samples from 15 retrofaryngeal lymphnodes (55.5 percent), 9 prescapular lymphnodes (33.3 percent), 2 lungs (7.4 percent), and 1 liver (3.7 percent). All isolated colonies were confirmed by PCR to contain MTBC organisms, and were identified as M. bovis by the same methodology.


Subject(s)
Animals , Cattle , Mycobacterium bovis/isolation & purification , Mycobacterium tuberculosis/isolation & purification , Tuberculosis, Bovine/microbiology , Bacterial Typing Techniques , Brazil , DNA, Bacterial/analysis , Mycobacterium bovis/genetics , Mycobacterium tuberculosis/genetics , Polymerase Chain Reaction
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