ABSTRACT
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.
Subject(s)
Phloroglucinol/pharmacology , Cryopreservation/methods , Cryoprotective Agents/pharmacology , Musa/cytology , Rhizome/cytology , Reference Values , Sucrose/pharmacology , Time Factors , Reproducibility of Results , Plant Shoots/drug effects , Plant Shoots/physiology , Musa/drug effects , Rhizome/drug effects , Glycerol/pharmacologyABSTRACT
Os autores apresentam um estudo retrospectivo de 200 pacientes operados no Hospital Saúde, pelo mesmo cirurgiao, de colecistite calculosa. Desses pacientes, 100 foram operados pela técnica convencional e os demais pela técnica laparoscópica. Foram analisados: tempo cirúrgico e de hospitalizaçao; necessidade de analgésicos potentes e complicaçoes pós-operatórias. Esse estudo permitiu concluir a superioridade, estatisticamente significativa, da cirurgia laparoscópica em relaçao à convencional quanto ao tempo cirúrgico, tempo de hospitalizaçao, necessidade de analgésicos potentes e índice de morbidade.