ABSTRACT
Immunoglobulin E [IgE] mediated allergy is a common chronic disorder resulting from interaction between genetic and environmental factors. We aimed to reveal the single nucleotide polymorphisms' [SNPs] in 1L-18 [137G>C] and CD 14 [C-159T] genes to reveal their possible association with atopic diseases in Egyptian population. A total of 30 diagnosed atopic patients and a total of 30 age-matched control subjects were included in this study. Diagnosis of atopy was made according to the family history and the results of both intradermal skin test and total IgE enzyme- linked immunosorbent assay. Interleukin-18 [-137G>C] genotyping was performed by polymerase chain reaction with sequence specific primers [PCR-SSP] The CD14 [C-159T] polymorphism was detected by polymerase chain reaction -Restriction fragment length polymorphism [PCR-RFLP], Insignificant higher prevalence of heterozygous genotype [GC] of IL-18 [-137G>C] gene was found in atopic patients compared to control subjects. Statistical analysis revealed significant association between atopy and the CC genotype of CD14 [C-159T]. Only the genotype combination GG/ CC which is composed of the IL-18 GG genotype and CD 14 CC genotype was preferentially transmitted to atopic patients. The results of this study suggested a possible association of atopy with the CD 14 [C-159T]. Moreover, the genotype combination GG/ CC may be one of the factors that participate in the. pathogenesis of atopy?
ABSTRACT
Enterobacter infection was investigated in 880 patients attending both Inpatient and Outpatient Clinics of Zagazig University Hospitals. Enterobacter was isolated from 95 clinical samples [10.8%]. It accounted for lower respiratory infection in 38, unrinary tract infection in 21, wound infection in 25, burn sepsis in 4 and bacteraemia in 3 patients. The duration of hospitalization greatly affected the isolation rate. Most of the strains [75.8%0 were from patients who spent more than 5 days in the hospital especially those who had a history of antimicrobial therapy. Amongst different sepcies E. Cloacae was the most frequently isolated. All cases of Enterobacter infection were directly proportionate to age. We confirmed the role of diabetes and other underlying medical or surgical problems especially genitourinary obstruction and instrumentation as predisposing factors. Our hospital isolates peaked each summer