Cytologically diagnosed metastatic small cell lung carcinoma in the mandibular soft tissue

Metastatic tumors to the oral and maxillofacial region are relatively rare, they constitute 1% of all malignant tumors of the oral cavity. The purpose of this case report is to evaluate the efficiency of fine needle aspiration cytology [FNAC] in the diagnosis of metastatic small cell lung carcinoma. A 50-year-old female patient presenting with a 4x5 cm firm, hemorrhagic, tender swelling on the left mandibular bicuspid gingiva was evaluated. Her past medical history revealed a mass measuring 8x5 cm in the left pulmonary hilar area with pleural effusion, which was diagnosed as small cell lung carcinoma. A FNAC was performed to the oral mucosal swelling, and cytological examination revealed metastatic small cell lung carcinoma. The duration between diagnosis of the primary lung and development of metastasis was 6 months. The FNAC is a rapid, non-invasive, and safe diagnostic method when carried out with a proper technique, and proved to be a valuable adjunct to a careful physical and radiological examination of the oro-maxillofacial lesions

Keratocystic odontogenic tumor: case report with CT and ultrasonography findings

Keratocystic odontogenic tumor (KCOT) is a benign odontogenic tumor with a potentially aggressive and infiltrative behavior. KCOT is most commonly occurred in mandible and demonstrate a unilocular, round, oval, scalloped radiolucent area, while large lesions may appear multilocular. An important characteristic of KCOT is its propensity to grow in an antero-posterior direction within medullary cavity of bone causing minimal expansion. Definitive diagnosis relies on histological examination. In this report, a KCOT that had an expansion both buccal and lingual cortical bone is described including its features in computed tomography and ultrasonographic exams. The lesion was removed surgically via an intraoral approach under local anesthesia and histologically reported as a KCOT.

Stimulation of bone formation by direct electrical current in an orthopedically expanded suture in the rat / 대한치과교정학회지

OBJECTIVE: The aim of this experimental study was to evaluate the effects of direct electrical current stimulation (DECS) on bone regeneration in response to an expansion of the inter-premaxillary suture in the rat. METHODS: Sixteen 50 - 60 days old Wistar male rats were separated into two equal groups (control and experimental). Both groups were subjected to expansion, and 30-gram of force was applied to the maxillary incisors with helical-spring. In the experimental group, two metallic-screws were placed at lateral parts of the maxillary segments. Electrodes were connected to the screws. The device was activated with current adjustment to measure 10 microA continuously and the current was monitored daily during the expansion and early-retention phase. Bone regeneration in the sutural area was histomorphometrically evaluated including new-bone area (micrometer2), bone perimeter (micrometer), feret's diameter (micrometer) and newly formed bone (%) parameters. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p < 0.05 level. RESULTS: Statistical analysis showed significant differences between groups for all investigated histomorphometric parameters. New bone area (p = 0.002), bone perimeter (p = 0.004), feret's diameter (p = 0.002) and newly formed bone percentage (p = 0.002) measurements were significantly higher in the experimental group than the control group. Bone histomorphometric measurements revealed that bone architecture in the DECS group was improved. CONCLUSIONS: The application of DECS to an orthopedically expanded inter-premaxillary suture area during the early retention phase stimulated the formation of new bone.

Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat / 대한치과교정학회지

OBJECTIVE: The aim of this study was to evaluate the effects of vitamin E (alpha-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically. METHODS: Thirty 50 - 60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of alpha-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg alpha-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p<0.05 level. RESULTS: New bone area, bone perimeter, feret's diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p<0.001). Bone architecture in alpha-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner. CONCLUSIONS: The application of alpha-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.

Investigation of proliferative activity in the developing human tooth using Ki-67 immunostaining

The aim of this study was to investigate the proliferation of the developing human tooth germ and its surrounding tissues using Ki-67 immunostaining. Sections of mandibular dental arch tissues collected from 4 cadaveric human fetuses of 13, 16, 21 and 30 weeks of gestation were used. The immunoreactivity of Ki-67 in the tissue sections was assessed visually under a light microscope. Immunohistochemical controls were performed by replacing the primary antibody with phosphate-buffered saline or normal rabbit lgG. The control sections did not display Ki-67 immunoactivity. Specimens of 13 weeks of gestation revealed intense Ki-67 immunostaining throughout the entire developing mandibular primary molars. At 16 weeks of gestation, immunostaining was observed in the inner enamel epithelium and dental papilla, in conjunction with the dental lamina showing decreased immunostaining. At 21 weeks, Ki-67 immunostaining was observed only in the inner enamel epithelium and dental papilla. The immunoreactivity of active ameloblasts and odontoblasts decreased, along with the proliferation capacity of the dental lamina. At 30 weeks, both enamel and dentin formation was observed along the cusped aspect of the tooth germ. Ameloblasts and odontoblasts were no longer immunoreactive in this region, while both types of cells were immunoreactive at the cervical regions of the crown. Dental lamina cells showed disintegration and were totally Ki-67-negative at 30 weeks of gestation. The Ki-67 immunoreactivity of the dental lamina decreased during intrauterine tooth development. Positive immunostaining was observed at specific sites in the enamel organ and dental papilla during the cap and bell stages

Impact of C-erb2 status on survival after high dose chemotherapy in high-risk breast cancer patients

To investigate the impact of c-erb2 status on survival after high-dose chemotherapy. Between March 1997 and June 2004, a total of 54 women with breast cancer who has at least 8 metastatic lymph nodes underwent high-dose chemotherapy with hematopoietic stem cell transplantation in Giilhane Military Medical School, Ankara, Turkey. Archival specimens were analyzed by fluorescent in situ hybridization to determine the impact of c-erb2 status after peripheral blood stem cell transplantation on survival. The patients were divided into c-erb2 negative [n=20] and positive [n=l 1] groups. No statistically significant differences were detected between c-erb2 negative and positive groups regarding 5 -year disease-free survival [41 and 27%, log rank p=0.1 1], and overall survival [60 and 45%, p=0.33[. Transplant related mortality did not differ between groups. We found no differences between c-erb2 negative and positive groups regarding disease-free and overall survival. To clarify the value of the c-erb2 status in predicting outcome after high-dose chemotherapy, prospective randomized studies are needed