Vaccination against fascioliasis using homologous and Schistosoma mansoni derived glutathione S-transferases

Schistosoma mansoni glutathione S-transferase [SmGST] and Fasciola gigantica glutathione S-transferase [FgGST] were purified from adult worms homogenate by affinity chromatography. An assessment of both SmGST and FgGST as candidate vaccines was done by comparing vaccinated guinea pigs groups challenged with F. gigantica metacercariae with the control group in terms of worm load, pathological changes in liver tissue and the survival rate of the animals. A decrease in mortality rate was best reported with FgGST 15 ug dose, while no significant decrease in worm load was reported with any vaccination. The pathological changes in liver tissue of vaccinated and non- vaccinated animals were similar

antigenic fractions of strongyloides stercoralis filariform larval crude soluble antigen

This study was carried out on the sera of fifteen Strongyloidiasis patients, twenty-five cases with other helminthic infections and five cases free from parasitic infections [control]. Enzyme immunoblot transfer assay [EIBT] was used to assess IgG response against the different fractions of Strongyloides stercoralis filariform larval crude soluble antigen separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis [SDS-PAGE]. In the immunoblot, all sera of 15 strongyloidiasis patients disclosed IgG antibodies reactivity against immunogenic bands having molecular weights. Five immunodominant antigenic bands of 66, 50, 41, 38 and 31 KDa were commonly recognized by the strongyloidiasis sera IgG suggesting that these antigenic components may be suitable for specific immunodiagnosis of human strongyloidiasis