ABSTRACT
The objective of the study was to detect the presence of fimH geneamong the drug resistanst strains of Acinetobacter baumannii.fimH gene was found to be associated with a catch bond mechanism which led to better evolution of biofilm formation. Since there are not many studies done with this gene it would be a timely investigation and this study mainly aims in molecular characterizationof fimHgene among clinical isolates of A.baumannii. Semi quantitative bio adherent assay was done by the multidrug resistant strains of A.baumannii to find the formation of biofilm. The DNA was extracted with the help of kit and PCR was performed for amplification. Pearson correlation analysis was done to find the existing correlation between the fimHgene and MDR strains of A.baumanniiwith significant p-value of (<0.05). From the screened 73 genomes of MDR A.baumannii 6.8% showed positive amplicons for the fimH gene which were related to biofilm and porin formation (Fig. 1). Correlation of its existence was high in beta lactamase (100%), cephems (100%), folate (100%) resistant strains, followed by aminoglycosides (80%), carbapenems (60%) and fluoroquinolones (60%) and efflux pumps (20%). In Spite of various measures undertaken to prevent the disease, the prevalence of the pathogen is multiplying. The current study recorded the presence of fimHgene (6.8%) among the clinical isolates of A.baumannii. This gene can be used as a target to develop new drugs and vaccines to combat the menace of A.baumannii infection
ABSTRACT
Liquid supported denture can be a permanent solution in edentulous patients with diabetes, xerostomia and atrophied ridge. A liquid-supported denture was planned for maxillary arch and neutral zone concept for fabrication of contour of the polished surfaces of mandibular denture. Liquid-supported dentures will have optimal stress distribution during masticatory function. The neutral zone technique provides muscular harmony over the denture stability. Liquid supported denture will act as a continuous reliner for the denture and thus has an advantage over the conventional denture. Neutral zone technique is an alternative approach for the construction of mandibular dentures where there is atrophic ridge and a history of denture instability.
ABSTRACT
Selenium administration resulted in a marked decrease in the activity levels of the liver succinate dehydrogenase, malate dehydrogenase, and lactate dehydrogenase while pyruvate dehydrogenase increased significantly (P<0.001) in the wistar rat. The degree of decrease of these enzymes was significantly less (P<0.001) when rats were treated with curcumin, a natural constituent Curcuma longa. Curcumin seems to prevent oxidative damage mediated through selenium and protect the dehydrogenases possibly through its anti-oxidative property.
Subject(s)
Administration, Oral , Animals , Antioxidants/administration & dosage , Curcumin/administration & dosage , Enzyme Inhibitors/administration & dosage , L-Lactate Dehydrogenase/antagonists & inhibitors , Liver/drug effects , Malate Dehydrogenase/antagonists & inhibitors , Male , Rats , Rats, Wistar , Selenium/toxicity , Succinate Dehydrogenase/antagonists & inhibitorsABSTRACT
Wistar rat pups treated with curcumin, a natural constituent of Curcuma longa before being administered with selenium showed no opacities in the lens. The lipid peroxidation, xanthine oxidase enzyme levels in the lenses of curcumin and selenium co-treated animals were significantly less when compared to selenium treated animals. The superoxidase dismutase and catalase enzyme activities of curcumin and selenium co-treated animal lenses showed an enhancement. Curcumin co-treatment seems to prevent oxidative damage and found to delay the development of cataract.