Ornamental fishing in the region of Barcelos, Amazonas: socioeconomic description and scenario of activity in the view of piabeiros / A pesca ornamental na região de Barcelos, Amazonas: descrição socieconômica e cenário da atividade na visão dos piabeiros

Abstract The Negro river basin is considered the largest area of extractive of ornamental fish in Brazil. This area has fundamental importance for the populations from the Amazon. The present study aimed to describe socioeconomic profile of ornamental fishermen known as "piabeiros" in the Municipality of Barcelos, as well as the ornamental fishery, fisheries area, target species, environments, fishing techniques, equipment, capture techniques and main difficulties faced by the current activity. This study was carried out in municipality of Barcelos, through semi - structured interviews, with artisanal ornamental fishermen (N= 89). The main families of ornamental fish caught and traded were: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae and Gymnotidae. The main catchment areas were igarapés, lakes, flooded fields, beaches, river banks and igapó forest. Rapiché was the most used equipment in the fisheries both by the fishermen of the urban areas (43.81%) and rural (41.89%). Most of the fishermen are associated with the colony of fishermen of Barcelos (Z33). The data showed that the dynamics of ornamental fishing have changed in a short time and directly affected fishermen, in addition to the low age renewal with the participation of younger fishermen, threatening the transmission of ecological knowledge to future generations. As a result, the increase of the problems related to the productive chain and absence of public power to the activity, since ornamental fishing has already been treated as one of the main economic activities more important for the local communities and for the State of Amazonas.

Resumo A bacia do rio Negro é considerada a maior área de extrativismo de peixes ornamentais do Brasil. Esta área possui importância fundamental para as populações da Amazônia. O presente estudo objetivou descrever o perfil socioeconômico de pescadores conhecidos como "piabeiros" no município de Barcelos, bem como a pesca ornamental, área de pesca, espécies-alvo, ambientes, técnicas de pesca, equipamentos, técnicas de captura e dificuldades enfrentadas nesta atividade. O estudo foi realizado por meio de entrevistas semi - estruturadas, com pescadores artesanais ornamentais (N= 89). Os dados mostram que as famílias de peixes ornamentais capturados e comercializados foram: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae e Gymnotidae. As áreas de captura descritas foram igarapés, lagos, alagados, praias, margens de rios e floresta de igapó. O Rapiché foi o equipamento mais utilizado nas pescarias tanto pelos pescadores das áreas urbanas (43,81%) como rurais (41,89%). A maioria dos pescadores está associada à colônia de pescadores de Barcelos (Z33). Os dados coletados mostraram que a dinâmica da pesca ornamental mudou em pouco tempo e afetou diretamente os pescadores. Além da baixa taxa de renovação, com a participação de pescadores mais jovens, ameaçando a transmissão de conhecimento ecológico para as gerações futuras. Como resultado, notamos o aumento dos problemas relacionados à cadeia produtiva e a ausência de poder público na atividade. A pesca ornamental já foi tratada como uma das principais atividades econômicas para as comunidades locais e para o Estado do Amazonas.

Transaxillary single-port subtotal parathyroidectomy: feasibility study in cadavers / Paratireoidectomia subtotal transaxilar single-port: estudo de viabilidade em cadáver

ABSTRACT Objective : to test the minimally invasive technique of single-port transaxillary subtotal parathyroidectomy in non-formalized cadavers to evaluate its viability and reproduction. Method : we performed ten subtotal parathyroidectomies through a transaxillary TriPort access in cadavers. The technique consisted of access through the axillary fossa, creating a subcutaneous tunnel to the anterior cervical region, for handling of the thyroid gland and dissection and resection of the parathyroid glands. Results : all surgeries were successful. The mean time of surgery was 65 minutes (57-79 min), with uncomplicated identification of all anatomical structures. There was no need for complementary incisions in the cervical region. Conclusion : the transaxillary single-port subtotal parathyroidectomy technique was feasible and reproducible, suggesting an alternative for minimally invasive cervical surgery.

RESUMO Objetivo: testar a técnica minimamente invasiva de paratireoidectomia subtotal transaxilar single-port em cadáveres não formalizados para avaliar sua viabilidade e reprodução. Método: foram realizadas dez paratireoidectomias subtotais por via transaxilar através de acesso por TriPort em cadáveres. A técnica realizada consistiu em acesso pela fossa axilar, criando-se um túnel subcutâneo até a região cervical anterior, para manuseio da glândula tireoide e dissecção e ressecção das paratireoides. Resultados: todas as cirurgias foram realizadas com sucesso. O tempo médio de cirurgia foi 65 minutos (57-79 min), com identificação, sem dificuldades, de todas as estruturas anatômicas. Não houve necessidade de incisões complementares na região cervical. Conclusão: a técnica de paratireoidectomia subtotal transaxilar single-port foi viável e reprodutível, sugerindo uma alternativa para a cirurgia cervical minimamente invasiva.

Ornamental fishing in the region of Barcelos, Amazonas: socioeconomic description and scenario of activity in the view of piabeiros

Abstract The Negro river basin is considered the largest area of extractive of ornamental fish in Brazil. This area has fundamental importance for the populations from the Amazon. The present study aimed to describe socioeconomic profile of ornamental fishermen known as piabeiros in the Municipality of Barcelos, as well as the ornamental fishery, fisheries area, target species, environments, fishing techniques, equipment, capture techniques and main difficulties faced by the current activity. This study was carried out in municipality of Barcelos, through semi - structured interviews, with artisanal ornamental fishermen (N= 89). The main families of ornamental fish caught and traded were: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae and Gymnotidae. The main catchment areas were igarapés, lakes, flooded fields, beaches, river banks and igapó forest. Rapiché was the most used equipment in the fisheries both by the fishermen of the urban areas (43.81%) and rural (41.89%). Most of the fishermen are associated with the colony of fishermen of Barcelos (Z33). The data showed that the dynamics of ornamental fishing have changed in a short time and directly affected fishermen, in addition to the low age renewal with the participation of younger fishermen, threatening the transmission of ecological knowledge to future generations. As a result, the increase of the problems related to the productive chain and absence of public power to the activity, since ornamental fishing has already been treated as one of the main economic activities more important for the local communities and for the State of Amazonas.

Resumo A bacia do rio Negro é considerada a maior área de extrativismo de peixes ornamentais do Brasil. Esta área possui importância fundamental para as populações da Amazônia. O presente estudo objetivou descrever o perfil socioeconômico de pescadores conhecidos como piabeiros no município de Barcelos, bem como a pesca ornamental, área de pesca, espécies-alvo, ambientes, técnicas de pesca, equipamentos, técnicas de captura e dificuldades enfrentadas nesta atividade. O estudo foi realizado por meio de entrevistas semi - estruturadas, com pescadores artesanais ornamentais (N= 89). Os dados mostram que as famílias de peixes ornamentais capturados e comercializados foram: Characidae, Lebiasinidae, Gasteropelecidae, Cichilidae, Anostomidae, Loricaridae, Potamotrygonidae e Gymnotidae. As áreas de captura descritas foram igarapés, lagos, alagados, praias, margens de rios e floresta de igapó. O Rapiché foi o equipamento mais utilizado nas pescarias tanto pelos pescadores das áreas urbanas (43,81%) como rurais (41,89%). A maioria dos pescadores está associada à colônia de pescadores de Barcelos (Z33). Os dados coletados mostraram que a dinâmica da pesca ornamental mudou em pouco tempo e afetou diretamente os pescadores. Além da baixa taxa de renovação, com a participação de pescadores mais jovens, ameaçando a transmissão de conhecimento ecológico para as gerações futuras. Como resultado, notamos o aumento dos problemas relacionados à cadeia produtiva e a ausência de poder público na atividade. A pesca ornamental já foi tratada como uma das principais atividades econômicas para as comunidades locais e para o Estado do Amazonas.

Establishment of an anesthetic protocol for semen collection by electroejaculation in six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758) / Estabelecimento de um protocolo anestésico para coleta de sêmen por eletroejaculação em tatus-peba (Euphractus sexcinctus Linnaeus, 1758)

The aim was to verify the effects of different anesthetic protocols used during electroejaculation (EEJ) in six-banded armadillos (Euphractus sexcinctus). Four sexually matured animals were physically restrained and subjected to semen collection by the EEJ following three treatments: The control group consisted of no use of anesthesia; in the others, the anesthetic combinations xylazine/ketamine/propofol or butorphanol/ ketamine/propofol were administered. For each group, twelve procedures were conducted for EEJ. Semen was evaluated for volume, color, aspect, motility, sperm concentration, morphology, viability, and functional membrane integrity. The highest efficiency (100% ejaculates) was achieved when the control group was used; the xylazine/ketamine/propofol association provided only 11 ejaculates from a total of 12 attempts (91.6% efficiency), while only 4 ejaculates (33% efficiency) were obtained with butorphanol/ketamine/propofol (P<0.05). Both protocols provided rapid induction and relaxation enough to perform the EEJ. In the use of butorphanol/ketamine/propofol, the animals recovered at 16.5±1.5min, a time shorter than in the use of xylazine/ketamine/propofol protocol, 20.7±1.0min (P>0.05). The semen volume and sperm concentration obtained in the use of xylazine/ketamine/propofol association were significantly higher than those verified for butorphanol/ketamine/propofol protocol. In conclusion, the xylazine/ketamine/propofol association is indicated for anesthesia of six-banded armadillos submitted to EEJ.(AU)

Objetivou-se verificar os efeitos de diferentes protocolos anestésicos usados durante a eletroejaculação (EEJ) em tatus-peba (Euphractus sexcinctus). Quatro animais sexualmente maduros foram contidos fisicamente e submetidos à coleta de sêmen por EEJ, seguindo três tratamentos: o grupo controle consistiu do não uso de anestesia; nos outros, foram administradas combinações anestésicas de xilazina/cetamina/propofol, ou butorfanol/cetamina/propofol. Para cada grupo, foram conduzidos 12 procedimentos de EEJ. O sêmen foi avaliado para volume, cor, aspecto, motilidade, concentração de espermatozoides, morfologia, viabilidade e integridade funcional da membrana. A mais alta eficiência (100% de ejaculados) foi alcançada quando o grupo controle foi utilizado; a associação de cetamina/xilazina/propofol forneceu apenas 11 ejaculados de um total de 12 tentativas (de eficiência 91,6%), enquanto apenas quatro ejaculados (eficiência de 33%) foram obtidos com butorfanol/cetamina/propofol (P<0,05). Ambos os protocolos forneceram rápida indução e relaxamento suficientes para executar a EEJ. Na utilização de butorfanol/cetamina/propofol, os animais se recuperaram em 16,5±1,5min, um tempo mais curto do que no uso de xilazina/cetamina/protocolo de propofol, 20,7±1,0min (P>0,05). O volume de sêmen e a concentração espermática obtidos no uso da associação xilazina/cetamina/propofol foram significativamente maiores do que os verificados para o protocolo butorfanol/cetamina/propofol. Em conclusão, a associação de cetamina/xilazina/propofol é indicada para anestesia de tatus-peba submetidos à EEJ.(AU)

High-impact exercise in rats prior to and during suspension can prevent bone loss

High-impact exercise has been considered an important method for treating bone loss in osteopenic experimental models. In this study, we investigated the effects of osteopenia caused by inactivity in femora and tibiae of rats subjected to jump training using the rat tail suspension model. Eight-week-old female Wistar rats were divided into five groups (n=10 each group): jump training for 2 weeks before suspension and training during 3 weeks of suspension; jump training for 2 weeks before suspension; jump training only during suspension; suspension without any training; and a control group. The exercise protocol consisted of 20 jumps/day, 5 days/week, with a jump height of 40 cm. The bone mineral density of the femora and tibiae was measured by double energy X-ray absorptiometry and the same bones were evaluated by mechanical tests. Bone microarchitecture was evaluated by scanning electron microscopy. One-way ANOVA was used to compare groups. Significance was determined as P<0.05. Regarding bone mineral density, mechanical properties and bone microarchitecture, the beneficial effects were greater in the bones of animals subjected to pre-suspension training and subsequently to training during suspension, compared with the bones of animals subjected to pre-suspension training or to training during suspension. Our results indicate that a period of high impact exercise prior to tail suspension in rats can prevent the installation of osteopenia if there is also training during the tail suspension.

Analysis of fracture healing in osteopenic bone caused by disuse: experimental study

Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail suspension, when the osteopenic condition was already set. The fractured limb was then immobilized by orthosis. Tibias were collected 2 and 6 weeks after osteotomy, and were analyzed by bone densitometry, mechanical testing, and histomorphometry. Bone mineral density values from bony calluses were significantly lower in the 2-week post-osteotomy groups compared with the 6-week post-osteotomy groups (multivariate general linear model analysis, P<0.000). Similarly, the mechanical properties showed that animals had stronger bones 6 weeks after osteotomy compared with 2 weeks after osteotomy (multivariate general linear model analysis, P<0.000). Histomorphometry indicated gradual bone healing. Results showed that osteopenia did not influence the bone healing process, and that time was an independent determinant factor regardless of whether the fracture was osteopenic. This suggests that the body is able to compensate for the negative effects of suspension.

Rendimento do coentro (Coriandrum sativum L) adubado com esterco bovino em diferentes doses e tempos de incorporação no solo / Yield of the coriander [Coriandrum sativum (L)] fertilized with manure at different doses and incorporation times in the soil

RESUMO O uso de esterco bovino é de suma importância para os agricultores familiares que produzem coentro na região de Mossoró-RN, pois esse insumo é amplamente disponível e utilizado pelos agricultores. Objetivando-se avaliar o Rendimento do coentro fertilizado com esterco bovino em diferentes doses e tempos de incorporação ao solo foi conduzido um experimento no período de setembro a novembro de 2011, na Fazenda Experimental Rafael Fernandes, da Universidade Federal Rural do Semi-Árido - UFERSA, Brasil. Os tratamentos consistiram da combinação de quatro doses de esterco bovino incorporadas ao solo: 15,0; 30,0; 45,0 e 60,0 t ha-1 em base seca, com quatro tempos de incorporação: 28; 49; 64 e 80 dias antes da semeadura do coentro - DAS, mais um tratamento controle (ausência de adubação). O delineamento experimental utilizado foi em blocos completos casualizados com os tratamentos arranjados em esquema fatorial 4 x 4 + 1 com 3 repetições. A cultivar de coentro utilizado foi a Verdão e as variáveis determinadas foram altura e número de hastes por planta e rendimento de coentro. O coentro respondeu à aplicação de esterco bovino, produzindo rendimentos máximos de 6453 e 6349 kg ha-1 de massa verde, com a dose de 60 t ha-1 e aos quarenta e seis dias de incorporação antes da semeadura, respectivamente.

ABSTRACT The use of manure is very important to family farmers who produce coriander in the region of Mossoró-RN, because this input is widely available and used by farmers. Aiming to evaluate the yield of the coriander fertilized with manure at different doses and incorporation times in the soil, a trial was conducted from September to November 2011, at the Experimental Farm Rafael Fernandes in the Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró-RN, Brazil. The treatments consisted on the combination of four levels of manure incorporated into the soil: 15.0; 30.0; 45.0 and 60.0 t ha-1 at dry basis, with four incorporation times: 28; 49; 64 and 80 days before the coriander"s sowing - DAS, plus a control treatment (without fertilization). The experimental design was a randomized complete block with treatments arranged in a 4 x 4 + 1, with three replications. The coriander planted was the "Verdão" and the specific variables were height and number of stalks per plant and yield of the coriander. The coriander responded to the application of the manure, producing maximum yields of 6453 and 6349 kg ha-1 of fresh mass, with the dose of 60 t ha-1 and at forty-six days of incorporation before the sowing, respectively.

Origem e distribuição do nervo femoral do mocó, Kerodon rupestris (Cavidae) / Origin and distribution of femoral nerve of the rocky cavy, Kerodon rupestris (Cavidae)

O mocó (Kerodon rupestris Wied,1820), um mamífero roedor da família Cavidae, que se assemelha bastante ao preá, é um animal altamente adaptado às condições de calor e de escassez de água e de alimento, principalmente nos períodos das grandes secas que assolam periodicamente a região do semi-árido nordestino. Verifiica-se que na literatura há escassez de dados referentes à anatomia funcional dos mocós, em especial de trabalhos envolvendo a anatomia do sistema nervoso. Visando conhecer a origem do nervo femoral junto aos forames intervertebrais, sua localização e distribuição pelo membro pélvico, a musculatura envolvida em seu trajeto, a importância desse estudo para clínica de animais silvestres e contribuir para o desenvolvimento da neuroanatomia comparada, procedeu-se esta pesquisa, na qual foram utilizados dez animais adultos de diferentes idades (4 machos e 6 fêmeas) que vieram a óbito no Centro de Multiplicação de Animais Silvestres (Cemas) da Universidade Federal Rural do Semi-Árido (Ufersa). Os animais foram fiixados em solução aquosa de formaldeído a 10 por cento e posteriormente tiveram a cavidade abdominal dissecada até a completa visualização do nervo femoral. Foram verifiicadas variações no número de vértebras lombares nos animais, entre seis (30 por cento) e sete (70 por cento) vértebras, alterando, conseqüentemente, a origem do nervo. No antímero direito, verifiicou-se que em 40 por cento dos animais o nervo femoral originava-se de ramos ventrais de L5L6, em 40 por cento de L5L6L7 e em 20 por cento de L4L5L6. Já no esquerdo 50 por cento dos exemplares o nervo femoral foi formado de raízes ventrais de L5L6, em 30 por cento de L5L6L7 e em 20 por cento de L4L5L6.

Rock cavy (Kerodon rupestris Wied, 1820), a rodent mammal from the Cavidae family is highly adapted to conditions of heat and shortage of water and food, mainly in the periods of great drought that periodically devastate the semi-arid of Northeast Brazil. In the literature, few data are found regarding the functional anatomy of the rock cavy, especially involving the anatomy of the nervous system. We aimed to investigate the origin of the femoral nerve close to the intervertebral foramina, its location and distribution for the musculature of the legs, to verify its importance to support further studies for wild animal clinics, and to contribute for the comparative neuro-anatomy. Ten adult rock cavies of different ages were used (4 males and 6 females), that had died in the Wild Animal Multiplication Center (Cemas) of the Rural Federal University of the Semi-Arid, Mossoró. After the fixation in aqueous solution of 10 percent formalin, the dissection of the abdominal cavity of the animals was accomplished for complete visualization of the femoral nerve. Variations were verified in the number of lumbar vertebrae, as seven animals (70 percent) had seven lumbar vertebrae, and three (30 percent) only six, altering the origin of the nerve. On the right side, in four animals (40 percent) the femoral nerve originated from ventral branches of L5 to L6, four (40 percent) from L5 to L7, and in two (20 percent) from L4 to L6. On the left side, in five animals (50 percent) the femoral nerve originated from the ventral branches of L5 to L6, in three (30 percent) from L5 to L7, and two (20 percent) also from L4 to L6.

Qualidade fisiológica de sementes de coentro [Coriandrum sativum (L. )] / Physiologic quality of cilantro seeds [Coriandrum sativum (L. )]

A qualidade das sementes esta relacionada de forma direta ao seu potencial fisiológico, representado pela germinação e/ou vigor, expressando sua capacidade de originar plântulas normais. O objetivo do estudo foi a análise dos efeitos da temperatura e do tempo de exposição ao teste de envelhecimento acelerado sobre os parâmetros: germinação, germinação na primeira contagem, emergência, Índice de velocidade de emergência, comprimento de parte aérea e massa seca. O ensaio foi conduzido no delineamento inteiramente casualizado, com quatro repetições. As sementes foram submetidas à dois métodos de envelhecimento acelerado: o tradicional com água destilada e outro com solução saturada de 40 g NaCl 100 mL-1 de água, em duas temperaturas (38 ºC e 41 ºC) e em três períodos de exposição (48, 72 e 96 horas). Melhor se pode verificar o potencial fisiológico das sementes de coentro, quando submetido às condições estressantes: na temperatura de 41 °C com o período de exposição de 96 h (Teste envelhecimento acelerado tradicional) e na temperatura de 41 ºC com período de exposição de 48 h (com solução saturada). Os testes de germinação e primeira contagem evidenciaram diferenças na qualidade fisiológica inicial das sementes em função dos tratamentos aplicados.

The quality quality of the seeds this related of direct form to it physiologic potential, acted by the germination and/or energy, expressing it capacity to originate normal plantules. The objective of the study was the analysis of the effects of the temperature and of the time of exhibition to the test of accelerated aging on the parameters: germination, germination in the first counting, emergency, Index of emergency speed, length of aerial part and mass dries. The rehearsal was driven in the delineamento entirely casualizado, with four repetitions. The seeds were submitted to two methods of accelerated aging: the traditional with distilled water and other with saturated solution of 40 g NaCl 100 mL-1 of water, in two temperatures (38 ºC and 41 ºC) and in three exhibition periods (48, 72 and 96 hours). Better the physiologic potential of the cilantro seeds can be verified, when submitted to the stressful conditions: in the temperature of 41 °C with the period of exhibition of 96 h (it Tests traditional aging) and in the temperature of 41 °C with period of exhibition of 48 h (with saturated solution). The germination tests and first counting evidenced differences in the quality physiologic initial of the seeds in function of the applied treatments.

Estudo de características de produção de matrizes de corte por meio da análise de componentes principais / Study of meat-type chickens production traits by principal components analysis

Avaliou-se o descarte de variáveis de produção, em análises de componentes principais, de três linhagens de matrizes de corte do Programa de Melhoramento Genético da Universidade Federal de Viçosa, utilizando informações de 270 aves, sendo 90 de cada linhagem. As características analisadas foram dias para postura do primeiro ovo (DPPO), taxa de postura da 22ª a 56ª semana (TP), peso médio individual na 32ª (PMI1), na 40ª (PMI2), na 48ª (PMI3), na 56ª (PMI4) e na 64ª semana (PMI5) e peso médio do ovo, obtido pela média da pesagem de três ovos na 32ª (PMO1), na 40ª (PMO2), na 48ª (PMO3), na 56ª (PMO4) e na 64ª semana (PMO5). Dos 12 componentes principais, sete apresentaram variância menor do que 0,7 (autovalor menor do que 0,7), sugerindo-se sete variáveis para descarte. As variáveis descartadas foram aquelas que apresentaram maiores coeficientes, em valor absoluto, a partir do último componente principal. Observou-se correlação linear simples e significativa entre as variáveis descartadas e as não descartadas, que indica redundância de variáveis, razão do descarte. Recomendam-se as variáveis: DPPO, TP, PM14, PMO1 e PMO4 para o estudo de características da produção de matrizes de frango de corte por meio da análise de componentes principais.

Records of 270 meat-type chickens from three lines, 90 of each one, were used to discard variables in a principal component analysis. Data were obtained from meat-type chicken lines of the genetic breeding program of the Universidade Federal de Viçosa. The following traits were evaluated: days at first egg (DFE), egg production rate (EPR) from 22nd to 56th week, body weights at 32nd (BW1), 40th (BW2), 48th (BW3), 56th (BW4), and 64th weeks of age (BW5), and average of three egg weights, at 32nd (EW1), 40th (EW2), 48th (EW3), 56th (EW4) and at 64th weeks (EW5). From the 12 principal components, seven showed variance lower than 0,7 (eigenvalue lower than 0,7), suggesting seven variables to be discarded. Variables which showed the highest coefficients, in absolute value, in the last principal component were discarded. Highly correlated variables with the smaller principal components variance explain a small part of the whole variation. In addition, discarded variables in function of the significant simple linear correlation with the nondiscorded variable, were considered redundants. The variables DFE, EPR, BW4, EW1, and EW4 are recommended for principal component analysis of broiler matrix.

Eficácia dos coadjuvantes de higiene bucal utilizados na alimentação de cães / Effectiveness of buccal hygiene coadjutant used in the dogs feeding

Avaliou-se a utilização de coadjuvantes na diminuição da placa bacteriana e formação do cálculo dentário em 16 cães. O delineamento experimental foi casualizado com quatro tratamentos (T) e quatro repetições. Os tratamentos foram: T1- controle, T2- coadjuvante com ação mecânica, T3- coadjuvante com tripolifosfato de sódio e T4- coadjuvante com hexametafosfato de sódio. Após sete dias de adaptação, no dia anterior ao início dos tratamentos, os animais foram submetidos à remoção de cálculo dentário. O experimento teve duração de 21 dias e ao final realizaram-se as medições das placas bacterianas formadas com o uso de marcadores (fucsina). O coadjuvante somente com ação mecânica não foi efetivo em retardar o aparecimento da placa bacteriana. Os coadjuvantes com polifosfatos apresentaram uma ação efetiva e significativa na diminuição da formação do calculo dentário

The use of coadjutants in the decrease of the bacterial plate and formation of the dental calculus was evaluated in 16 dogs. The experimental design was randomized with four treatments (T) and four repetitions. The treatments were the following: 1- control; 2- coadjutant 1 (one) with mechanical action; 3- coadjutant 2 (two) with tripolyphosphate of sodium and 4- coadjutant 3 (three) with hexametaphosphate of sodium. After seven days of adaptation, on the day previous to the beginning of the treatments, the animals were submitted to the removal of dental calculus and after twenty-one days of treatment a measurement of the bacterial plaque formed through the use of markers (fucsin). The coadjutant only with mechanical action was not effective in delaying the appearance of the bacterial plaque. The coadjutant containing polyphosphates presented an effective and significant action decreasing the formation of the dental calculus

Functional expression of kinin B1 and B2 receptors in mouse abdominal aorta

Previous studies have shown that the vascular reactivity of the mouse aorta differs substantially from that of the rat aorta in response to several agonists such as angiotensin II, endothelin-1 and isoproterenol. However, no information is available about the agonists bradykinin (BK) and DesArg9BK (DBK). Our aim was to determine the potential expression of kinin B1 and B2 receptors in the abdominal mouse aorta isolated from C57BL/6 mice. Contraction and relaxation responses to BK and DBK were investigated using isometric recordings. The kinins were unable to induce relaxation but concentration-contraction response curves were obtained by applying increasing concentrations of the agonists BK and DBK. These effects were blocked by the antagonists Icatibant and R-715, respectively. The potency (pD2) calculated from the curves was 7.0 ± 0.1 for BK and 7.3 ± 0.2 for DBK. The efficacy was 51 ± 2 percent for BK and 30 ± 1 percent for DBK when compared to 1 æM norepinephrine. The concentration-dependent responses of BK and DBK were markedly inhibited by the arachidonic acid inhibitor indomethacin (1 æM), suggesting a mediation by the cyclooxygenase pathway. These contractile responses were not potentiated in the presence of the NOS inhibitor L-NAME (1 mM) or endothelium-denuded aorta, indicating that the NO pathway is not involved. We conclude that the mouse aorta constitutively contains B1 and B2 subtypes of kinin receptors and that stimulation with BK and DBK induces contractile effect mediated by endothelium-independent vasoconstrictor prostanoids.

Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells

A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization

Lipomatose difusa e congenita de extremidades. / Congenital and diffuse lipomatosis of extremities

Sao apresentados cinco casos de lipomatose difusa e congenita de extremidades cursando com gigantismo de dedos e aumento de volume de mao e pe correspondentes. O estudo radiologico mostrou aumento de volume das falanges dos dedos envolvidos, ao lado de rarefacao ossea e de lesoes osteoliticas. Histologicamente, documentou-se infiltracao difusa de plano muscular, de pele e de osso pelo tumor