ABSTRACT
Background: The aim of the study is focussed very keenly at performing RCA (root cause analysis) of those particular sample containing the absurd results of the Serum UIBC (Unsaturated Iron Binding Capacity), which was also tallied vigilantly side by side with Serum Iron observed at Clinical Biochemistry laboratory of The New Civil Hospital Surat, Gujarat.Methods: The Absurd value from the samples requested for Serum Iiron and Serum Serum UIBC (Unsaturated Iron Binding Capacity) from month of August 2018 till the month of August 2019 were taken in to account for analysis. RCA (root cause analysis) of absurd value for Serum Iron and Serum UIBC (Unsaturated Iron Binding Capacity) which were prepared was mainly focused on tallying with Serum Calcium and Serum Potassium result.Results: With the continious and strenous monitoring from the side of the researchers, the researchers had throughly analysed and found that in almost all of those analysed test containing absurd results of Serum Iron and Serum UIBC(Unsaturated Iron Binding Capacity) which were some way or the other, associated with absurd results of Serum Calcium along with absurd result of Serum Potassium too from the same samples.Conclusions: The Absurd results of Serum Calcium and Serum Potassium are caused mainly due to pre-analytical errors more likely due to the sample contamination with EDTA (ethylene diamine tetra acetic acid). The contaminated EDTA (ethylene diamine tetra acetic acid) sample cause interference in Serum Iron measurement by producing turbidity in sample and in Serum UIBC (Unsaturated Iron Binding Capacity) by chelating Iron.
ABSTRACT
We describe a case of 46 years lady with dermatomyositis and calcinosis cutis showing extensive subcutaneous nodules with fluid discharge. The discharge fluid contained very high calcium concentration. X-ray of the subcutaneous nodules showed calcification of soft tissue. USG of spleen showed foci of vascular calcification.
ABSTRACT
Aims: The study aims at comparing observed values of glucose and various common clinical chemistry examinations in sample containers with different glycolysis inhibitor – Glyceraldehyde. Materials And Methods: Prepare sample conainers with alternate glycolysis inhibitors.Collect blood samples in prepared sample containers (systematic sampling of 100 consenting patients admitted at various wards of New civil Hospital,Surat for each perservative).Observe values of various examinations in collected samples. Statistical Analysis Used: Perform two-sample Student's t-test on observed sets of data. Results: Average Glucose concentration in 5 mmol/L DL-Glyceraldehyde, Fluoride and Plain tube each was 118.78 mg/dl, 121.84 mg/dl and 100.24 mg/dl respectively after 8 hours. P-value for glyceraldehyde tube vs. Fluoride tube was 0.64737, P-value for glyceraldehyde tube vs. Plain tube was 0.00796 and P-value for Fluoride tube vs. Plain tube was 0.00187. P-value for Bilirubin, ALT,ALP, Amylase,Electrolytes, Triglycerides, Cholesterol, HDL Cholesterol, Urea, Uric Acid, Albumin, Total Proteins were unaffected by DL-Glyceraldehyde, while there was negative interferance with Creatinine measurement by Alkaline Picrate method. Conclusions: Use of DL-Glyceraldehyde containers for preservation of blood for Glucose measurement is as good as fluoride containers. Because Glyceraldehyde interfere with creatinine assay by Alkaline Picrate method and pottasium assay by ISE , Glyceraldehyde containers can not replace Plain tube for common routine clinical chemistry analysis.