ABSTRACT
A herbal hepatoprotective formulation Liv 52 down regulated the tumour necrosis factor (TNF) production in Charles Foster Rats treated with CCl4. Inhibition of TNF activity was proportional to the hepatoprotective activity.
Subject(s)
Animals , Carbon Tetrachloride Poisoning/metabolism , Down-Regulation/drug effects , Drug Combinations , Chemical and Drug Induced Liver Injury/metabolism , Plant Extracts/pharmacology , Plants, Medicinal , Rats , Tumor Necrosis Factor-alpha/biosynthesisABSTRACT
Administrations of hepatotoxicants namely carbon tetrachloride (CCl4:0.4 ml in 1.2 ml of liquid paraffin) and ANIT (1 ml of 1.5% solution in liquid paraffin) in Charles foster rats (force fed) and D-galactosamine (8 mg in water per swiss albino mouse, ip) induce the release of TNF-alpha in case of CCl4 and D-galactosamine. High TNF-alpha level was observed up to 48 hr in CCl4 and up to 24 hr in D-galactosamine treated animals. Elevated levels of biochemical like ALP and SGPT are also recorded. TNF-alpha level can be measured of tissue damage and prognosis in case of hepatitis.
Subject(s)
1-Naphthylisothiocyanate/pharmacology , Alanine Transaminase/blood , Alkaline Phosphatase/blood , Animals , Carbon Tetrachloride/pharmacology , Galactosamine/pharmacology , Liver/drug effects , Mice , Rats , Tumor Necrosis Factor-alpha/biosynthesisABSTRACT
'Arogyavardhini'-an indigenous formulation was evaluated for its hepatoprotective activity in rats, using two models of carbon tetrachloride (CCl4) hepatic damage, one simulating vital hepatitis and the other simulating fatty change. The protective effect was assessed from serum aspartate transaminase (AST) and alkaline phosphatase levels and from histopathological changes in liver. The results revealed that 'Arogyavardhini' (5 mg/100g, PO daily) was effective in minimizing the changes in serum levels of AST and alkaline phosphatase induced by CCI. The protective effect was also evident on histopathological examination.