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Chemotherapy is one of the most important methods of cancer treatment.However,multidrug resistance (MDR) has been the main factors affecting their efficacies.Recent studies show that the amplification of MDR1 gene in tumor cells,the over expression of the related drug resistance protein and the cell cycle and apoptosis pathway in signal transduction are the main causes for the failure of chemotherapy.
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To build up total,perfect,scientific,feasible teaching quality monitoring system in sub-colleges,and further fully explore efficient monitoring method,technique is an important measure of ensuring teaching quality under the background of expansion of recruitment.This paper discusses total teaching quality monitoring system from guiding ideology,basic content,inplementing regulations of monitoring system.
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According to teaching practice,the authors discuss the problems on teaching exploratory experiment of experimental physiology sciences and propose some reflection and suggestions.
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AIM: To explore the effect of EBV infection on growth and apoptosis of nasopharyngeal carcinoma(NPC) cell line.METHODS: NPC cell line CNE1 was directly infected by Epstein Barr virus (EBV). The expression of EBV-latent membrane protein 1 (EBV-LMP1) and bcl-2 were detected by immunohistochemistry method (LSAB). The growth of NPC cells was identified by MTT method. Apoptotic carcinoma cells were detected by flow cytometry analysis and the terminal deoxynucletidyl transferase-medicated dUTP-biotin nick end labeling (TUNEL) methods. RESULTS: EBV-LMP1 was positive in CNE1 infected by EBV(E-CNE1). Compared with CEN1, the growth of E-CNE1 apparently increased ( P
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AIM: To investigate the possible effect of different Latent membrane protein 1 (LMP1) variants on proliferation characteristics of a human well-differentiated nasopharyngeal carcinoma (NPC) cell line CNE1. METHODS: The plasmids which carried EBV-LMP1 gene cloned from B95-8 lymphocyte (B95-8-LMP1) and NPC tissues (CAO-LMP1) were introduced into CNE1 by liposomal transfection. Expression of LMP1 in CNE1 was identified by RT-PCR and Western blot, respectively. Different Effects of the two EBV-LMP1 variants on proliferation characteristics of CNE1 including growth curve, cell cycle distribution and clony-forming efficiency were investigated by means of crystal violet staining proliferation assay, flow cytometry and plastic plate clony-forming assay, respectively. RESULTS: Two transfected cell lines stably expressing different LMP1 variants were established successfully. Either of the two LMP1 variants increased CNE1 growth rate, proliferation index (PI) and clony-forming rate significantly and CAO-LMP1 had more significant growth-promoting effect on CNE1 than B95-8-LMP1. CONCLUSION: It can be concluded from the study that CAO-LMP1 promotes CNE1 proliferation more effectively than B95-8-LMP1. [
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AIM: To study the expression of p53 protein in nasopharyngeal carcinoma cells(NPC) after the telomerase activity being inhibited. METHODS: After transfecting telomerase RNA antisense oligodeoxynucleotides (asODN) into NPC CNE1 and CNE2Z cells by lipofectin, the proliferation of cells was identified by MTT method, apoptosis was examined by flow cytometric cell cycle analysis, and the expression levels of human teolmerase reverse transcriptase(hTRT) and p53 protein were detected by immunohistochemistry. RESULTS: asODN inhibited the growth of CNE1 and CNE2Z cells in a dose-dependent manner, meanwhile, the apoptotic peaks were detected with flow cytometer.The expression levels of hTRT and p53 protein in asODN group were significantly lower than those in other treatment groups and control group( P
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AIM: To observe the effect of protein kinase C-?(PKC?)antisense oligonucleotide on cell growth, cell cycle and the expression of cyclin E in human poor-differentiated nasopharyngeal carcinoma(NPC) cell line CNE-2Z. METHODS: Antisense PKC? was transfected by cationic liposome(LP) in CNE-2Z cells to analyze the cell growth and cell cycle by MTT colorimetric assay and flow cytometry, respectively. Moreover, the expression of cyclin E was determined by immunocellularchemistry and scanning the result of dot-blotting. RESULTS: ①With the concentration of antisense PKC? increasing, the relative cell growth index was decreased gradually( P
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Objective To study the effect of matrine on proliferation and metastasis associated the abilities of human highly metastatic ovarian cancer HO-8910PM cells and its possible mechanism.MethodsThe trypan blue staining and MTT assay were used to examine the effect of matrine on proliferation of HO-8910PM cells;Transwell chamber assay was performed to determine the effect of matrine on the invasive and migratory abilities of the cells;Coomassie brilliant blue staining assay was used to detect the changes in cytoskeleton treated by matrine at different concentrations and immunocytochemical methods were used to observe the expression of Ezrin protein in HO-8910PM cell line befores and after matrine treatment.Results There was an obvious dose-dependent and time-consuming effect on the inhibition of HO-8910PM cells proliferation by matrine;1.5 g/L matrine inhibited cell proliferation significantly,for 6 h after the treatment in vitro,the invasive and migratory abilities of HO-8910PM cells were also obviously down-regulated with the inhibitory rates(41.52?10.76)% and(39.73?10.67)%,respectively;Matrine distinctly changed the cytoskeleton and up-regulated the expression of Ezrin protein in HO-8910PM cell line treated with 1.0 g and 1.5 g/L matrine for 24 h.Conclusion Matrine inhibits the proli-feration,migration,and invasion abilities of HO-8910PM cell line in vitro,which may associate with the changes of cytoskeleton and Ezrin protein expression.
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AIM:To investigate the effect of Epstain-Barr virus latent membrane protein 1 (EBV-LMP1) on proliferation and cell cycle of nasopharyngeal carcinoma (NPC) cells. METHODS:The expression of EBV-LMP1 was detected by immunohistochemical method (LSAB). Proliferation of NPC cells was identified by MTT method. Cell cycle percentage was detected by flow cytometry analysis. RESULTS: OD value of EBV-LMP1 expressive NPC cells L-CEN1 was much higher than that of both EBV-LMP1 negative NPC cells V-CEN1 and CNE1 ( P 0.05).CONCLUSION:The expression of EBV-LMP1 on NPC cell might cause some change of cell cycle and enhance cell proliferation.
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AIM:To study the inhibitory effect and its mechanisms of the extract of Oratosquilla oratoria (EOS) on the activity of telomerase in human nasopharyngeal carcinoma cell line CNE-2Z. METHODS:MTT assay was used to determine the effect of different doses of EOS on the proliferation of CNE -2Z cells. The activity of telomerase was analyzed by TRAP-ELISA. The mRNA expression of hTERT was determined by RT-PCR,and the protein expression of c-Myc was detected by Western blotting. RESULTS:EOS inhibited the proliferation of CNE-2Z cells in a dose-dependent manner (P
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AIM: To investigate the effects of antisense oligonucleotides (asODN) of PKC-? and PKA-Ⅰon growth and proliferation of the CNE-2Z cells. METHODS: The expression of PKC-? and PKA-Ⅰ was observed with immunohistochemistry method. The asODNs of (1)PKC-?, (2)PKA-Ⅰ, (3)PKC-? and PKA-Ⅰ, were transfected into CNE-2Z cells by lipofectin (LP), and a random sequence as a control was used. The cell growth index (GI) and the clone formation rate of CNE-2Z were detected by MTT colorimetric assay and soft agar assy, respectively. RESULTS: The expression of PKC-? or PKA-Ⅰin CNE-2Z in experimental group were both significantly lower than that of control group(P