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OBJECTIVE:To establish a HPLC method for the content determination of chrysophanol in Yanbao Ⅱ capsule.METHODS:The separation was performed on Agilent C18(150 mm?4.6 mm,5 ?m) column with absolute alcohol-0.1% phosphoric acid (87:13) as mobile phase.The flow rate was 1.0 mL?min-1 and the detection wavelength was set at 254 nm.RESULTS:The linear range of chrysophanol was 0.042 6～0.340 8 ?g(r=0.999 9) with an average recovery of 99.39%(RSD=1.74%,n=6).CON-CLUSION:The method is simple,accurate,sensitive and reproducible for the quality control of YanbaoⅡ capsule.
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Objective To investigate the relationship factor of livability on Premature Very Low-Birth-Weight-Infant (PVLBWL), for refer to evidences that reduce the mortality and the deformity rate. Methods The group 116 PVLBWL cases, being in our hospital hear five years, divide into three team according to the weight, and analyze the review clinical data of the cases (from 1993.1 to 1998.1). Results There are 86 cases that had cured and left hospital in the group. The cure rate is 74.1%(86/116). It is rise along with the weight increase (P
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OBJECTIVE: To observe the effects of Shenqi bufei decoction on air passage and lung function of COPD model rats with lung-qi deficiency.METHODS: Quantitative stimulation with tobacco and SO2 and papain aerosol inhalation were used to establish model COPD rats with lung-qI deficiency.Sixty male rats were randomly divided into: normal group (N),model group(M),low dose treatment group(LT),medium dose treatment group (MT),high dose treatment group (HT),and glucocorticoid treatment group (GCT).Pathomorphological change of lung tissue in rats was observed with light microscope.The thickness of airway wall and smooth muscle layer of the small airway and lung function were measured by means of semi-quantitative image analysis system with lung function detection and analysis system respectively.RESULTS: As compared to N group,in M group the thicknesses of the small airway wall and smooth muscle layer were significantly increased(P