ABSTRACT
The biosynthetic pigment from Chromobacterium violaceum BB-78, 1,3-dihydro-2H-indol-2-one and its derivatives exhibit biological activities such as antimicrobial action, low hemolytic effects on red blood cells and in vitro trypanocide activity. A relatively high cytotoxicity on V-79 hamster fibroblast cells of the biosynthetic pigment was found, although with the methylol derivative the toxicity was almost eliminated. The methylol derivative exhibited similar toxicity as Nifurtimox, a known, commercial trypanocide compound
Subject(s)
Animals , Cricetinae , Anti-Bacterial Agents/toxicity , Chromobacterium/metabolism , Hemolysis , Indoles/toxicity , Pigments, Biological/toxicity , Anti-Bacterial Agents/isolation & purification , Bacteria/drug effects , Cell Line , Cell Survival/drug effects , Chromobacterium/growth & development , Fibroblasts/drug effects , Indoles/isolation & purification , Microbial Sensitivity Tests , Nifurtimox/toxicity , Pigments, Biological/isolation & purification , Trypanosoma cruzi/drug effects , Trypanosoma cruzi/growth & developmentABSTRACT
It has been found that the pigment-I from Chromobacterium violaceum, 3 [1,2 dihydro 5 (5 hydroxy 1H indol 3 yl) 2 oxo 3H pyrrol-3 ylidiene] 1,3 dihydro 2H indol 2 one, has trypanocide activity. The formylated derivatives, pigment-III, immobilized 100% of the Trypanosoma cruzi at a level of 46 micronM after 48h of interaction with a total growth inhibition in the same period. Pigment I exhibited low toxicity and a DNA synthesis inhibition similar to that of Nifurtimox, a known trypanocide compound