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@#AIM: To observe the changes of retinal structure in macular region of wet age-related macular degeneration(wARMD)after treatment with conbercept by optical coherence tomography(OCT), and explore the effect of conbercept on wARMD.<p>METHODS: From May 2018 to October 2018, 21 patients(23 eyes)with wARMD were studied. All patients were treated by intravitreal injection of conbercept monthly for 3mo. During the six-month follow-up period after the last injection, the changes of best corrected visual acuity(BCVA)and intraocular pressure were observed, in the meantime, the macular fovea retinal thickness(CFT), macular fovea pararetinal thickness(PMT), choroidal neovascularization(CNV)area and subretinal fibrosis also were observed by OCT.<p>RESULTS:Six months after treatment, 21 eyes(91%)was effective, and 2 eyes(9%)was stable in all patients. 1mo, 3mo and 6mo after treatment, the BCVA of patients were significantly improved, the areas of CFT, PMT and CNV decreased significantly compared with those of before(<i>P</i><0.05), while intraocular pressure did not change significantly before and after treatment(<i>P</i>>0.05). During the follow-up period, transient blurred vision occurred in 2 eyes(9%), intraocular pressure increased(>21mmHg)in 1 eye(4%)and subconjunctival hemorrhage in 1 eye(4%)of 23 eyes, and all recovered within 7d after the end of 3 conbercept treatments.<p>CONCLUSION: The application of conbercept in the treatment of wARMD can significantly improve the patients' vision and retinal structure in macular region. And it also shows good therapeutic effect, less side effects and high safety.
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Objective: To study the constituents from the dried aboveground part of Artemisia annua. Methods: The chemical constituents were isolated by various chromatographic techniques and their structures were elucidated by spectroscopic analyses and comparison of NMR data with those reported in literatures. Results: Six compounds were obtained and characterized as artelignan (1), scopoletin (2), scoparone (3), chrysosplenol B (4), jaceidin (5) and mikanin (6). Conclusion: Compound 1 is identified as a new compound named artelignan.
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OBJECTIVE@#To study the effect of mogroside VI (MVI) on acute liver injury induced by sepsis in mice and its possible mechanisms. Methods A total of 60 male C57BL/6 mice were randomly divided into five groups: sham-operation, model, low-dose MVI (25 mg/kg), high-dose MVI (100 mg/kg), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) inhibitor (100 mg/kg MVI+30 mg/kg PGC-1α inhibitor SR-18292), with 12 mice in each group. Cecal ligation and puncture were performed to establish a mouse model of sepsis. The drugs were given by intraperitoneal injection after the model was established, once a day for 3 consecutive days. ELISA was used to measure the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Colorimetry was used to measure the levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in liver tissue. Hematoxylin-eosin staining was used to observe liver histopathological changes. Liver mitochondrial respiratory function was measured, and mitochondrial respiratory control rate was calculated. RT-PCR was used to measure the copy number of mitochondrial DNA (mtDNA) in liver tissue and the mRNA expression levels of PGC-1α, nuclear respiratory factor-1 (NRF-1), and mitochondrial transcription factor A (TFAM) in liver tissue. Western blot was used to measure the protein expression levels of PGC-1α, NRF-1, and TFAM in liver tissue.@*RESULTS@#Compared with the sham-operation group, the model group had significant increases in the serum levels of ALT and AST and the content of MDA in liver tissue (P0.05). The PGC-1α inhibitor SR-18292 significantly inhibited the intervention effect of high-dose MVI (P<0.05).@*CONCLUSIONS@#MVI can effectively alleviate acute liver injury caused by sepsis in mice, possibly by enhancing mitochondrial biosynthesis mediated by PGC-1α.
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Animals , Male , Mice , Liver , Mice, Inbred C57BL , Sepsis/drug therapy , TriterpenesABSTRACT
OBJECTIVE@#To investigate the clinical outcome of the patients with primary diffuse large B-cell lymphoma（DLBCL）.@*METHODS@#Clinical data of 148 patients with DLBCL in our hospital and cancer hospital from March 2006 to April 2016 were retrospectively analyzed. Kaplan-Meier analysis was used to estimate progression-free survival（PFS）and overall survival（OS）.@*RESULTS@#5-year OS and PFS rates were 85% and 69%，respectively. The survival analysis showed that 5-year OS rate of R-CHOP group was significantly higher than that of CHOP alone group（89% vs. 70%，P60 years），poor ECOG score（≥2），advanced stage（Ⅲ-Ⅳ），higher IPI score（≥3），CHOP alone and absence of radiotherapy related with poor survival rate. Furthermore，multivariate analysis showed that age>60 year significantly related with the worse OS.@*CONCLUSION@#Age >60 year is an important independent prognostic factor to predict worse OS or PFS.
Subject(s)
Humans , Antibodies, Monoclonal, Murine-Derived , Antineoplastic Combined Chemotherapy Protocols , Cyclophosphamide , Disease-Free Survival , Doxorubicin , Lymphoma, Large B-Cell, Diffuse , Prognosis , Retrospective StudiesABSTRACT
To observe the contribution of FoxO1 on its downstream molecules expression and function in Jurkat cells,Jurkat cells were infected with FoxO1 expression lentivirus and interference lentivirus to establish FoxO1-KLF2-S1P1 signaling pathways mod-el.After infection of 120 h,FoxO1,KLF2,S1P1 and CD62L mRNA levels and the protein expression of FoxO1,FoxO1-p and KLF2 were increased (P<0.05) in FoxO1-overexpression group.Converse results(P<0.05) were observed in the interference group.The proportions of S1P1+cells were increased in both groups.It was notably that S1P1+cells were decreased(P<0.05) in interference group after infection of 72 h.The proportion of CD62L+cells was increased(P<0.05) in overexpression group,it was decreased(P<0.05) in the interference group.This vitro experiments showed S1P1 and CD62L could be regulated by FoxO1 lentivirus,and provided an experimental basis for research about intracellular FoxO1-KLF2-S1P1 signaling pathways and cellular functions.
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Objective To investigate the synergistic antihypertensive effect of Dilong Jiangya Capsules on patients with essential hypertension with subclinical atherosclerosis of liver yang hyperactivity type and its effect on arteriosclerosis. Methods Totally 120 patients with essential hypertension with subclinical atherosclerosis were randomly divided into treatment group (60 cases) and control group (60 cases). Control group received antihypertensive treatment by following the individual, low-dose, long-term, combined principle, while treatment group was given Dilong Jiangya Capsules on the basis of control group, one capsule per time, twice a day, orally taken during early morning and bedtime. The treatment for both groups lasted for six weeks. Blood pressure, carotid artery intima-media thickness (IMT), pulse wave velocity (PWV) and ankle-brachial index (ABI) were observed before and 2, 4 and 6 weeks after treatment. Blood pressure clinical efficacy was evaluated. Results Two and three cases were lost in the treatment group and control group, respectively. The total effective rate was 91.38% (53/58) in the treatment group and 82.46% (47/57) in the control group, and the treatment group was significantly better than the control group (P<0.05). Compared with before treatment, the blood pressure of the two groups was significantly lower (P<0.05), and the treatment group was significantly lower than the control group (P<0.05). Compared with before treatment, the levels of IMT and PWV in both groups significantly decreased at 2 and 4 weeks of treatment, and ABI at 2, 4 and 6 weeks of treatment decreased significantly (P<0.05). There was statistical difference in IMT and PWV between the two groups at 4 weeks of treatment (P<0.05). There was statistical difference in ABI between the two groups at 2 and 4 weeks of treatment (P<0.05). There was statistical difference in IMT, PWV and ABI between the two groups at 6 weeks of treatment (P<0.05). Conclusion Dilong Jiangya Capsules combined with conventional antihypertensive treatment of essential hypertension with subclinical atherosclerosis can enhance the antihypertensive effect, and improve IMT, PWV and ABI to some extent.
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<p><b>OBJECTIVE</b>To investigate the value of blood lactic acid (BLA) in evaluating disease severity and prognosis in children with sepsis.</p><p><b>METHODS</b>A total of 484 children with sepsis were enrolled and divided into common sepsis group (n=310), severe sepsis group (n=105), and septic shock group (n=69). BLA level was measured before treatment, and the results of BLA re-examination after early fluid resuscitation were collected for children with septic shock and a BLA level of >2 mmol/L.</p><p><b>RESULTS</b>The BLA level increased with the increasing severity of sepsis. The analysis of the receiver operating characteristic curve showed that the cut-off value of BLA for the diagnosis of septic shock was 2.25 mmol/L, with a sensitivity of 82.6% and a specificity of 79.8%. The fatality rates in the BLA ≤1 mmol/L, BLA 1.1-2 mmol/L, BLA 2.1-4 mmol/L, and BLA >4 mmol/L groups were 8.5%, 9.4%, 27.2%, and 67.6%, respectively, and the risk of death in the BLA >4 mmol/L group was 22.4 times that in the BLA ≤1 mmol/L group. In children with septic shock who had a BLA level of >2 mmol/L before treatment and whose BLA levels were ≤2 mmol/L or >2 mmol/L after resuscitation, the fatality rates were 33.3% and 69.2%, respectively.</p><p><b>CONCLUSIONS</b>BLA can be used to evaluate disease severity and prognosis in children with sepsis, and a BLA level of 2.25 mmol/L has a high value in diagnosing septic shock. Early resuscitation helps BLA level return to normal and can improve the prognosis of children with septic shock.</p>
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Adolescent , Child , Child, Preschool , Female , Humans , Infant , Male , Lactic Acid , Blood , Prognosis , Sepsis , Blood , Mortality , Severity of Illness IndexABSTRACT
<p><b>OBJECTIVE</b>To explore the therapeutic efficacy for patients aged over 70 years with acute myeloid leukemia(non-APL).</p><p><b>METHODS</b>Clinical data of 19 acute myeloid leukemia patients aged over 70 years admitted in our hospital from March 2006 to April 2016 years were analyzed retrospectively. Among them 15 patients received priming regimen and 4 patients received best supportive treatment. The overall survival time between patients with priming regimen and patients with best supportine treatment was compared by Kaplan-Meier analysis. The prognostic factors were evaluated by using multivariate analysis. Out of the 19 patients, 9 were males and 10 were females, with median age of 75 years (70-84).</p><p><b>RESULTS</b>After 2 cycles of induction chemotherapy, the complete response rate was (7/15)46.6% and overall response rate was (11/15) 73%. Significant longer median survival time was observed in priming regimen group, compared with that in best supportive treatment group (11 months vs 2 months)(χ=17.077, P<0.001). The patients were well tolerant to side-effect of chemotherapy. Multivariate analysis showed that only receiving induction chemotherapy or not was the independent prognostic factor of the survival time(P<0.05), while the sex, age, ECOG index, CD34, HLA-DR, WBC count and ratio of blast cells in bone marrow were factor affecting the prognosis.</p><p><b>CONCLUSION</b>The priming regimen may be practicable and effective for the treatment of patients with acute myeloid leukemia aged over 70 years (non-APL) and prolong the survival time of these patients.</p>
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<p><b>OBJECTIVE</b>This study was aimed to investigate the effect of salinomycin combined with vincristine on the proliferation and apoptosis of Jurkat cells and its possible mechanisms.</p><p><b>METHODS</b>The proliferation of Jurkat cells was examined by CKK-8 assay. Flow cytometry was used to assess cellular apoptosis. Levels of BCL-2, caspase-3, and caspase- 8 were measured by Western blot.</p><p><b>RESULTS</b>The salinomycin or vincristine, either alone or in combination, inhibited the proliferation of Jurkat cells in a dose-dependent manner. Salinomycin combined with vincristine produced more obveous inhibition of cell proliferation than either compound used alone (P<0.05). Western blot analysis showed that the combined use of Sal and VCR reduced the expression of BCL-2 protein, and increased expression of caspase 3 and caspase 8 protein, more significantly. Furthermore, combination of Sal and VCR synergistally promoted apoptosis of the Jurkat cells (P<0.05).</p><p><b>CONCLUSION</b>The combination of salinomycin and vincristine synergistically inhibits proliferation and promotes apoptosis of T-cell acute lymphoblastic leukemia Jurkat cells.</p>
Subject(s)
Humans , Apoptosis , Caspase 3 , Caspase 8 , Cell Proliferation , Flow Cytometry , Jurkat Cells , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma , Pyrans , VincristineABSTRACT
Objective To study the early diagnostic value of N-terminal pro-brain natriuretic peptide (NT-pro-BNP) for acute lung injury/acute respiratory distress syndrome (ALI/ARDS) in children and its influence on prognosis.Methods Fifty-two children with ALI/ARDS were selected as the experiment group,and 36 children with cardiac disease merging dyspnea were selected as the control group.The serum levels of NT-pro-BNP were measured in the 2 groups.The pediatric critical illness score(PCIS),lung injury score (LIS) and 28-day mortality were analyzed in the experiment group.The serum level of NT-pro-BNP,and the different condition and prognosis were compared in 2 groups,and the difference of the NT-pro-BNP levels were compared in children with ALI/ARDS.Results The NT-proBNP levels in experiment group were significantly higher than the control group (P ＜ 0.05).But the NT-pro-BNP levels in children with cardiaogenic pulmonary edema were significantly higher than ALI/ARDS(P ＜ 0.05).The NT-pro-BNP levels of the children who died in 28 d,or with LIS ＞ 2.5 scores or PCIS≤70 scores were higher than that of children who were survived,or with LIS ≤ 2.5 scores or PCIS ＞ 70 scores (P =0.001,0.013,0.002).The NT-pro-BNP levels were negatively correlated with PCIS(P ＜ 0.05),and the NT-pro-BNP levels were positively correlated with the 28-day mortality and LIS(all P ＜ 0.05).Conclusions The measurement of serum NT-pro-BNP can help to evaluate the severity and prognosis of ALI/ARDS children.
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<p><b>OBJECTIVE</b>To analyze the variation of serum insulin levels in critically ill children and investigate the underlying mechanism and clinical significance to provide the basis for treatment.</p><p><b>METHOD</b>Totally 332 critically ill children admitted in pediatric intensive care unit (PICU) of Hunan Children's Hospital from Nov., 2011 to April, 2012 were studied. The high insulin group (n = 332) was defined as insulin levels within 24 h > 11.1 mU/L and was divided into 2 groups: mildly elevated group (n = 194): 11.10 - 33.30 mU/L, increased three times group (n = 138): > 33.3 mU/L. Insulin, C-peptide and blood glucose were measured within 24 hours after admission, on day 3 and 7. Other results of inflammatory markers, lactate, cardiac enzymes, amylase, pancreatic ultrasound, hepatic and renal function as well as indicators related to severity and prognosis were recorded after admission.</p><p><b>RESULT</b>The peak of insulin level was seen on day 1, then presented a downward trend and reached the normal level on day 7. The peaks of blood glucose and C-peptide level were seen on day 1 then declined, the levels on day 7 were still slightly higher than normal level. The insulin level on admission (41.47 ± 30.85) mU/L were positively correlated with lactic acid (2.29 ± 1.81) mmol/L and procalcitonin level (5.08 ± 6.70) ng/ml (r = 0.370, P = 0.000; r = 0.168, P = 0.002) (P < 0.01). The insulin level on admission in children with 1 organ failure (41.24 ± 22.60) mU/L or 2 or multiple organ failure (48.98 ± 22.17) mU/L was higher than that in children with non-organ failure (34.11 ± 29.84) mU/L (U = 1621.001, P = 0.000;U = 1300.000, P = 0.000) (P < 0.01). The insulin level on admission in death group (52.99 ± 32.34) mU/L was higher than that in survival group (32.85 ± 24.10) mU/L (U = 1585.000, P = 0.000) (P < 0.01). Ten cases in death group were complicated with pancreatic damage and the average insulin level on admission was (65.29 ± 50.53) mU/L.</p><p><b>CONCLUSION</b>The high insulin level was correlated with the degree of inflammatory response, ischemia and hypoxia. The high insulin level in critically ill children was relevant to the pancreatic damage, the severity of the disease, organ dysfunction, and evaluation of prognosis.</p>
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Blood Glucose , Metabolism , C-Peptide , Blood , Calcitonin , Blood , Calcitonin Gene-Related Peptide , Critical Illness , Insulin , Blood , Intensive Care Units, Pediatric , Multiple Organ Failure , Blood , Mortality , Pancreas , Metabolism , Pathology , Prognosis , Protein Precursors , Blood , SurvivalABSTRACT
<p><b>OBJECTIVE</b>To study the clinicopathologic features and expression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) in adrenocortical tumors.</p><p><b>METHODS</b>Forty-two cases of adrenocortical tumors operated at the Beijing Union Medical College Hospital during the period from July, 2001 to July, 2010 were retrospectively reviewed. Immunohistochemical study for EGFR and VEGF was carried out. The clinical information and follow-up data were analyzed.</p><p><b>RESULTS</b>The cases included 21 adrenocortical carcinomas (ACC) and 21 adrenocortical adenomas (ACA). Nine patients suffered from primary aldosterone syndrome, including 8 cases with ACA and 1 case with ACC. The average tumor size, tumor weight, and duration between disease onset and diagnosis in the 21 cases of ACC were 11.7 cm, 542 g and 8.5 months, respectively. This was in contrast to 3 cm, 9.8 g and 45.6 months, respectively in cases of ACA. Histologically, the WEISS score in all the 21 cases of ACA was ≤ 2 (average = 0.9). None of the ACC cases had score less than 4 (average = 6.6). The presence of sinus invasion correlated with tumor metastasis (P < 0.01). Immunohistochemical study showed that EGFR was expressed in 61.9% of ACC patients (13/21), whereas EGFR staining was mostly negative in ACA (except for weak staining in 5 cases and moderate staining in 1 case). The difference of EGFR expression between ACC and ACA was statistically significant (P = 0.030). On the other hand, the positive rate of VEGF in ACC was 71.4% (15/21), including 28.6% (6/21) with strong expression and 28.6% (6/21) with moderate expression. In contrast, the expression rate of VEGF in ACA was 30.0% (7/21), including 14.3% (3/21) with moderate expression. The difference of VEGF expression between ACC and ACA was statistically significant (P = 0.013). There was correlation between VEGF expression and venous invasion (P = 0.028). The average duration of survival in patients with ACC was shorter than that in ACA. The tumor weight in ACC also correlated with prognosis.</p><p><b>CONCLUSIONS</b>Tumor size, weight and presence of endocrine symptoms may help in the differential diagnosis between ACC and ACA. A WEISS score of ≥ 3 highly suggests ACC. The presence of sinus invasion is associated with metastasis. EGFR or VEGF expression may also be important in differentiating ACC from ACA.</p>
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Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Adrenal Cortex Neoplasms , Metabolism , Pathology , General Surgery , Adrenocortical Adenoma , Metabolism , Pathology , General Surgery , Adrenocortical Carcinoma , Metabolism , Pathology , General Surgery , Diagnosis, Differential , Follow-Up Studies , ErbB Receptors , Metabolism , Retrospective Studies , Survival Rate , Tumor Burden , Vascular Endothelial Growth Factor A , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the drug resistance status on HIV-1 patients who had been treated with highly active antiretroviral therapy (HAART) and those treatment-naive ones in Hubei province.</p><p><b>METHODS</b>Nested polymerase chain reaction (PCR) was used to amplify 2 kb DNA fragment in HIV pol gene from peripheral blood of the HIV infected patients and the PCR products were sequenced. The sequences were compared to the Stanford HIV drug resistance database.</p><p><b>RESULTS</b>Nineteen patients were treated with regimens composed of two Nucleoside Reverse Transcriptase Inhibitors (NRTIs) and one Non-Nucleoside Reverse Transcriptase Inhibitors (NNRTI), with 25 patients as treatment-naive. Some protease (PR) drug-resistant mutations were found in these samples, such as D30N (2.27%), D30G (2.27%), M46I (4.55%), M46N (2.27%), I47V (4.55%), I84V (4.55%), I84L (2.27%), N88S (2.27%) and L90S (2.27%) that all belonged to major drug-resistant but A71T (29.55%) belonged to minor resistance mutations Five treated patients were detected having mutations associated RT drug resistance: M41L (5.26%), A62V (5.26%),D67N (5.26%), L210W (5.26%), T215Y (15.79%); K103E (5.26%), K103N (10.53%), Y181C (5.26%), G190A (5.26%), K238N (5.26%), while five treatment-naive patients were detected to have had mutations associated RT drug resistance M184V (4%), K65N (4%), Y115M (4%), F116L (4%), M184I (4%), V179D (4%), G190R (4%).Some additional mutations were detected in RT whose role involve in drug resistance still remained unknown. F214L was positively associated with HAART treatment (P = 0.03).</p><p><b>CONCLUSION</b>Significant differences were found between drug resistance mutations to RTIs in treated and treat-naive patients in Hubei province,indicating that drugs had affected the occurrence of drug resistance mutations. At the same time, novel RT mutations F214L might be associated with HAART or some other drugs.</p>
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Humans , Antiretroviral Therapy, Highly Active , China , Drug Resistance, Viral , Genetics , Genotype , HIV Infections , Drug Therapy , HIV-1 , Genetics , Mutation , Reverse Transcriptase Inhibitors , Therapeutic UsesABSTRACT
Objective To study clinicopathologic significance of microvessel density(MVD)and the expres- sion of CD34 in nasopharyngeal carcinoma.Methods Using Elivision Plus immunohistochemistry method.50 cases of nasopharyngeal carcinoma and 15 cases of inflammation nasopharyngeal tissues were stained with CD34.Results In comparison with inflammation nasopharyngeal tissues MVD(9.23?1.84),the MVD in nasopharyngeal carcino- ma(21.92?7.80)was significantly higher(P