ABSTRACT
Objective: To observe the therapeutic effects of effective compounds which were extracted from TongMai. Methods: SD rats experimential model with MsPGN were induced by subcutaneously injection rabbit anti-rat thymocyte serum(ATS) and forced swim. The rats were respectively ig with different dose(1.2,0.6,0.3 g'kg-1) of effective compound of TongMai for 4 weeks. Then the proliferative degree of mesangium was examined by HE stain, PAS stain and immunfluorescent respectively. Mesagium area proportion in glomerular capillaries area was calculated using image analyzing system. The expression of PCNA in the glomeruli was examined by immunohis to chemistry. Results: The effective compounds of TongMai can control the hyperplasia of the glomemlar mesangial cells (GMG)and the mainifold of the mesangial matrix, and prevent the deposit of IgG in mesangium. The proportions in glomerular capillaries area in model control group were significantly higher than that in the treatment groups; The express of proliferative cell nuclear antigen (PCNA) were ascended either. Conclusion: The effective compound of TongMai can improve the damage s of pathology in glomerular.
ABSTRACT
[Objective] To observe the effect of combination of Rhizoma Coptidis (RC) and Fructus Evodiae (FE) in various proportions on rat models with heat syndrome. [Methods] Rat models with heat syndrome were set up by gastric infusion of Radix Codonopsis Pilosulae and Radix Astragali seu Hedysari. Fifty rats were allocated to five groups: normal control group (Group A), model group (Group B), model rats treated with RC and FE at the proportion of 6∶1 (Group C), model rats treated with RC and FE at the proportion of 2∶1 (Group D) and model rats treated with RC and FE at the proportion of 1∶1 (Group E). [Results] Ultrastructure of hepatic cells were improved in Group C, Group D and Group E. Indexes of heat syndrome such as temperature, serum corticosteroid (Cor) level and 17 - hydroxycorticosteroid (17-OHCS) content in homogenate of adrenal glands were decreased in Group C and Group D but stayed unchanged in Group E as compared with Group B ( P