ABSTRACT
From January to December 1998, nasopharyngeal aspirates were obtained from 482 children with acute respiratory infections attended in emergence department and wards of a teaching hospital in the city of Salvador, Brazil. The samples were tested for the presence of adenovirus by isolation in tissue culture and indirect immunofluorescence assay. Eleven adenoviruses were detected by both methods in the same clinical samples. Infections by adenovirus were observed during seven months of the year without association with rainy season. Genome analysis was performed on these 11 isolates. Species C was represented by serotypes 1, 2 and 5. Within species B, only serotype 7 (Ad7) was detected. Two genomic variants of Ad1, two variants of Ad2, one of Ad5, and one of Ad7 (7h) were identified. This is the first study of molecular epidemiology of adenovirus associated to acute respiratory infections in children living in Northeast Brazil, and contributes to a better understanding of adenovirus infections in the country.
Subject(s)
Child , Humans , Adenoviruses, Human , Adenovirus Infections, Human/virology , Respiratory Tract Infections/virology , Acute Disease , Adenovirus Infections, Human/epidemiology , Adenoviruses, Human/genetics , Adenoviruses, Human/immunology , Adenoviruses, Human/isolation & purification , Brazil/epidemiology , DNA, Viral/analysis , Fluorescent Antibody Technique, Indirect , Genotype , Nasopharynx/virology , Respiratory Tract Infections/epidemiologyABSTRACT
Os enterovirus foram investigados em secrecoes respiratorias coletadas de pacientes com infeccao respiratoria aguda (IRA), durante um periodo de sete anos (1985-1991), dentro de um estudo longitudinal da etiologia das IRAs. Neste estudo foram encontrados todos os virus que sao mais comumente associados com IRA. Entre os virus isolados, os enterovirus foram apenas menos frequentes que virus respiratorio sincicial, adenovirus e influenzavirus. Quarenta e cinco amostras de enterovirus foram isoladas de pacientes com infeccao do trato respiratorio superior ou inferior. Entre estes enterovirus isolados, trinta e uma amostras foram identificadas como poliovirus (n=18) e enterovirus nao polio (n=13) pelo teste de soroneutralizacao. As amostras de poliovirus isoladas foram classificadas como amostras vacinais do tipo 1 e 2. Dos treze enterovirus nao polio, doze foram identificados como echovirus sorotipos 1, 2, 7, 11, 19 e 31 e apenas um foi identificado como coxsackievirus B4
Subject(s)
Humans , Child , Child, Preschool , Enterovirus/isolation & purification , Enterovirus Infections/etiology , Respiratory Tract Infections/epidemiology , Acute Disease , Brazil , Longitudinal Studies , Population Characteristics , Socioeconomic Factors , Tropical Climate/adverse effects , Urban AreaABSTRACT
Quarenta amostras de Adenovirus tipo 7 foram analisadas por digestao com as enzimas de restricao BamHI, SmaI, EcoRI e HindIII. Estas amostras foram coletadas de pacientes apresentando doenca respiratoria aguda durante os anos de 1980 a 1991. Somente dois tipos genomicos foram encontrados: Ad7b e Ad7e, sendo que Ad7b (87,5 por cento) foi mais frequente que Ad7e (12,5 por cento). O tipo genomico Ad7e apareceu nos anos 1980, 1981 e 1983. Ad7b apareceu durante 1982 e seguiu sendo o unico tipo genomico encontrado de 1984 a 1991. Ambos os tipos genomicos foram responsaveis por infeccoes do trato respiratorio superior (ITRS) e inferior (ITRI), porem a proporcao ITRI/ITRS e mais alta para Ad7b (25/6) que para Ad7e (1/4)