ABSTRACT
This work was focused on the evaluation of parameters of oxidative stress (lipid peroxidation, total thiol groups, non-protein thiol groups, polyphenols and ascorbate) and endothelial adaptation by determining the nitric oxide (NO), high-sensitive C-reactive protein (HS-CRP) and ischemia-modified albumin (IMA) of patients with chronic renal insufficiency submitted to hemodialysis. It was seen a depletion of glutathione, polyphenols and reduced ascorbate, combined with the increase of oxidized ascorbate and lipid peroxidation stress. In addition, was verified the increase of levels of IMA in the plasma. NO showed an evident endothelial protective role due to reduction of levels of HS-CRP, however, did not contribute to increase the levels of IMA. Based on the results, it can be concluded that the patients evaluated in this study presented a clinical situation of oxidative stress. A new study focusing on the prevention of oxidative stress using different experimental models with antioxidants is valuable to improve the life quality of these patients.
ABSTRACT
This work is focused on the evaluation of the anti anti-inflammatory activity of fractionated extracts of Salvia officinalis in experimental model of peritonitis. The extract of Salvia officinalis was produced by maceration with ethanol and fractionated by increasing the polarity of the solvents from hexane to methanol in a thin layer chromatographic column with Silica Gel 60 as stationary phase. The acute induced-peritonitis assay was used as model of inflammation, were the fractionated extracts of S. officinalis were administered subcutaneously. After 4h of induction of inflammation the number of total circulating leukocytes was not increased in animals that received the methanol extract. After the induction of inflammation, all animals except those treated with the fraction of ethyl acetate and methanol showed an increased number of circulating neutrophils. The results obtained suggesting the occurrence of inhibition of the total leukocytes recruitment in the circulating blood after the induced-inflammatory process by ethyl acetate and methanolic extracts of S. officinalis in a single dose of 25 μg.kg-1. It was concluded that the extracts of S. officinalis could be used as anti-inflammatory agent.