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Objective:To investigate the expressions of O-GlcNAc transferase (OGT) in the hepatocellular carcinoma (HCC) tissue,cells and serum,and to clarify the significance of OGT in diagnosis and treatment of HCC.Methods:Western blotting method was used to detect the expression levels of OGT in 20 samples of HCC tissue and matched non-tumor tissue,8 hepatoma cell lines and normal liver (human) cell lysate.The expression levels of serum OGT were detected by direct ELISA in 20 liver cirrhosis (LC) patients,20 HCC patients and 20 healthy volunteers.The relationship between the expression levels of protein in HCC tissue,cells and serum and the occurrence and development of tumor were analyzed.Results:The Western blotting results showed there were expressions of OGT in both HCC tissue and matched non-tumor tissue;The positive expression rate of OGT in HCC tissue was significantly higher than that in non-tumor tissue (P＜0.05).There were expressions of OGT in both hepatoma cell lines and normal liver (human) cell lysate;the positive expression rate of OGT in HCC cell lysate was significantly higher than that in the normal liver cell lysate (P＜0.05).The result of direct ELISA showed that the serum OGT levels of the LC and HCC patients were equally higher than that of the healthy volunteers (P＜0.05).Conclusion:OGT highly expresses in HCC tissue and OGT is a promising biomarker for the diagnosis of HCC.
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Objective:To investigate the expressions of O-GlcNAc transferase (OGT) in the hepatocellular carcinoma (HCC) tissue,cells and serum,and to clarify the significance of OGT in diagnosis and treatment of HCC.Methods:Western blotting method was used to detect the expression levels of OGT in 20 samples of HCC tissue and matched non-tumor tissue,8 hepatoma cell lines and normal liver (human) cell lysate.The expression levels of serum OGT were detected by direct ELISA in 20 liver cirrhosis (LC) patients,20 HCC patients and 20 healthy volunteers.The relationship between the expression levels of protein in HCC tissue,cells and serum and the occurrence and development of tumor were analyzed.Results:The Western blotting results showed there were expressions of OGT in both HCC tissue and matched non-tumor tissue;The positive expression rate of OGT in HCC tissue was significantly higher than that in non-tumor tissue (P＜0.05).There were expressions of OGT in both hepatoma cell lines and normal liver (human) cell lysate;the positive expression rate of OGT in HCC cell lysate was significantly higher than that in the normal liver cell lysate (P＜0.05).The result of direct ELISA showed that the serum OGT levels of the LC and HCC patients were equally higher than that of the healthy volunteers (P＜0.05).Conclusion:OGT highly expresses in HCC tissue and OGT is a promising biomarker for the diagnosis of HCC.
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Chronic hepatitis B virus (HBV) infection is one of the major disease burdens worldwide. At present, the antiviral therapy for hepatitis B includes interferons and nucleos(t)ide analogues. Current therapeutic regimens based on these drugs cannot significantly increase the proportion of patients with functional cure. With a better understanding of HBV replication cycle and specific virus-host cell interactions, this article summarizes and reviews the advances in the research and development of new drugs for HBV with a focus on different action targets during the above processes.
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<p><b>OBJECTIVES</b>To study the interaction of beta-2-glycoprotein I (beta 2GPI) with the membrane of hepatocytes and determine whether beta 2GPI participates in HBV infection.</p><p><b>METHODS</b>Ligand blotting, fluorescence microscopy, and fluorescence activated cell sorter (FACS) analysis were used to detect the specific interaction of beta 2GPI with the hepatoma cell line smmc7721, the gastric carcinoma cell line SGC7901, and the lymphoma cell line HL-60.</p><p><b>RESULTS</b>A specific 40 kDa beta 2GPI band was observed by ligand blotting in the case of smmc7721 cells. No such band was observed in SGC7901 or HL-60 cells. Fluorescence microscopy also revealed specific binding of FITC-beta 2GPI to smmc7721 cells, but neither to SGC7901 nor HL-60 cells. FACS analysis demonstrated that the binding rate of FITC-beta 2GPI to smmc7721 cells was significantly higher than these in SGC7901 and HL-60 cells (P < 0.01). The binding rate to smmc7721 cells did not increase with increasing amounts of FITC-beta 2GPI.</p><p><b>CONCLUSIONS</b>There is a specific beta 2GPI-binding protein on the membrane of hepatoma cells in cell line smmc7721 which as the beta 2GPI receptor may participate in HBV infection of hepatocytes.</p>
Subject(s)
Animals , Carcinoma, Hepatocellular , Cell Membrane , Metabolism , Flow Cytometry , Glycoproteins , Metabolism , Hepatocytes , Metabolism , Liver Neoplasms, Experimental , Metabolism , Platelet Glycoprotein GPIb-IX Complex , Platelet Membrane Glycoproteins , Tumor Cells, Cultured , beta 2-Glycoprotein IABSTRACT
<p><b>OBJECTIVE</b>To clarify the binding character between Beta-2-glycoprotein I (Beta-2-GPI) and HBsAg.</p><p><b>METHODS</b>Beta-2-GPI was purified from human plasma and labelled with biotin. Solid phase enzyme linked absorbance assay was used to investigate its binding with HBsAg.</p><p><b>RESULTS</b>Biotinylated Beta-2-GPI was found to bind HBsAg and the reaction could be inhibited by excess unlabelled Beta-2-GPI.</p><p><b>CONCLUSIONS</b>Beta-2-GPI may play a role in hepatitis B virus infection.</p>
Subject(s)
Humans , Binding Sites , Biotinylation , Enzyme-Linked Immunosorbent Assay , Methods , Glycoproteins , Metabolism , Hepatitis B Surface Antigens , Metabolism , Hepatitis B virus , Chemistry , Metabolism , beta 2-Glycoprotein IABSTRACT
Objective To explore the level and clinical significance of combination rate between beta2-glycoproteinⅠand platelet in the ulcerative colitis patients.Methods For 67 ulcerative colitis(UC) patients from the First and the Second Hospital of Jilin University from the September 2003 to December 2004,using flow cytometry(FCM),we detected combination rates of between beta2-glycoproteinⅠand platelet in the UC patients and in the normal control subjects,respectively.Results There was significant difference in the combination rates of beta2-glycoproteinⅠand platelet in the two UC groups and normal control group(P
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Objective:To compare the survival rate and side effects of doxorubic in (ADM) liposome therapy and ADM therapy for liver cancer.Methods:87 patients with advanced liver cancer were divided into two groups:Group A was treated by ADM liposome chemotherapy and group B by ADM chemotherapy.Results:The mean survival and 1 year survival rates showed no obvious difference in both groups.However,patients in Group A showed better immediate curative effect and lower immediate side effects than Group B.Conclusion:ADM Liposome chemotherapy increase the immediate curative effects and decrease the general reactions.
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Objective To study the relationship between anti-? 2-glycoprotein I(a? 2GPⅠ) and extracellular matrix (ECM) in patients with post-hepatitis B cirrhosis (PHBC).Method The of patients with chronic hepatitis B (CHB) and PHBC were studied for the serum a? 2GPⅠ levels by ELISA with purified ? 2GPⅠ as antigen, and we investigated serum level of collagen IV (IV-C)?hyaluronic acid (HA) and laminin (LN) in these patients.Result High positive rate was observed compared with control group (P
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Objective To investigate the relationship between some autoantibodies including anti-? 2-glycoprotein Ⅰ (anti-? 2GPⅠ) and chronic liver disease. Methods A cohort of patients with chronic hepatitis B (CHB) and post-hepatitis B cirrhosis (PHBC) was studied for the serum anti-? 2GPⅠ levels by ELISA with purified ? 2GPⅠ as antigen. The serum dsDNA, smooth muscle antibody (SMA) and ribonucleoprotein antibody (RNPA) were also detected. Results High positive rate was observed in patients with CHB or PHBC (20.9%, 9/43; 49.3%,35/75) comparing with that in control group (3.1%, 1/32) ( P