ABSTRACT
<p><b>OBJECTIVE</b>To investigate the properties of self-microemulsifying drug delivery system (SMEDDS) of effective parts from Jiaotai pills, and evaluate the character on improving intestinal absorption in rat.</p><p><b>METHOD</b>The appearance, the morphology, the particle size, tape, the time of formulating micro emulsion, the content and the stability were investigated; Taken total alkaloid as index, the intestinal absorption were investigated by using in situ recirculation model and comparing the micro emulsion solution and reference solution, The T (relative percentage remained) and K(a) (absorption rate constant) were calculated.</p><p><b>RESULT</b>After self-microemulsified, the average diameter of microemulsion was 34.12 nm and the time of self-microemulsifying was less than 3 min. SMEDDS was stable in room temperature in 3 months. The microemulsion were stable in 0.1 mol x L(-1) HCl at 37 degrees C for 8 h. T and K(a) of total alkaloid in SMEDDS were much higher than in that of its reference (P < 0.01), and the former K(a) was 152.6% of versus reference.</p><p><b>CONCLUSION</b>SMEDDS might significantly improve the absorption of both poorly soluble and low biological availability drugs in the intestinal tract, and increase drug stability, which will be suitable for compound Chinese medicine.</p>
Subject(s)
Animals , Rats , Administration, Oral , Dosage Forms , Drug Delivery Systems , Drug Stability , Drugs, Chinese Herbal , Chemistry , Pharmacokinetics , Emulsions , Intestinal Absorption , Particle Size , Rats, Sprague-DawleyABSTRACT
Objective To study the influence of the preparation and quality control of Xiaochuan cataplasm on its drug action. Methods Xiaochuan cataplasm was prepared with hydrosoluble polymers matrix. A sequence of tests such as adhesive force test, recontour test, paste-content test, cold-resistance test, heat-resistance test, toxicity and pharmacodynamic action tests were performed on this drug. Results Xiaochuan cataplasm contains a sound volume of medicine, and shows good adhesive property, no sensibilization and stimulation, and notable drug action. Conclusion The results demonstrate that the preparation craft of Xiao-chuan cataplasm is reasonable and practicable., the quality is controllable and the method is a safe and effective transdermal drug delivery system.
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Objective To select the optimum bases for Sihuang Gao and study the method for quality control.Method Ultra-fine powders of Radix Scutellariae,Cortex Phellodendri,Radix et Rhizoma Rhei and Rhizoma Coptidis were used to prepare Sihuang Gao with different formulations,the bacteriostatic action of which were compared and stability were inspected.The best prescription was identified by TLC,and the skin irritation was observed.Result Sihuang Gao made of water-soluble base had a strong antibacterial activity and stable quality,and it was proved no irritation,the contents of active component could be detected by TLC.Conclusion The quality was controllable,and the improvement prescription can be used in the clinic.
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Objective To develop an HPLC method for the assay of sinapine thiocyanate in Semen Sinapis Albae of different processed products. Methods It was assayed by RP-HPLC. The column was Hypersil BDS Phenyl (5 ?m, 250 mm?4.6 mm), the mobile phase was acetonitrile-0.08 mol/L KH2PO4 (15∶85). The UV detection wavelength was 240 nm. The flow rate was 1.0 mL/min. Results Good linearity of sinapine thiocyanate was showed within the range of 0.8～4.0 ?g (r=0.999 7). The average recovery was 100.98% (RSD=3.51%). Conclusion The method is simple, accurate and good reproducible. It can be used for quality control of Semen Sinapis Albae of different processed products.
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Objective To optimize the preparation process of Xiaochuan Tiegao. Methods The effective compound imporatorin of Dahurian Angelica Root was determined by HPLC. The best condition of preparation process for the ethanol-extraction method was investigated by L9(34) orthogonal design. Results The optimum technical condition:adding 2 times ethanol, cooking 1 hour and 2 times with 90% ethanol. Conclusion The method is reliable, accurate and easy to operate.