Comparison of ALS gene mRNA between sessile and planktonic Candida albicans of oral cavities / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To observe the different mRNA levels of Candida albicans ALS gene family between planktonic and biofilm-grown cells.</p><p><b>METHODS</b>ATCC 90038 and a wild strain of Candida albicans, biofilm models in vitro were formed on glass slides. After 48 hours' incubation, the biofilm-grown cells were harvested. Half-quantification of ALS1 and ALS4 mRNA was based on the amplification by one-step RT-PCR.</p><p><b>RESULTS</b>The amounts of ALS1 and ALS4 mRNA of the wild strain in biofilm increased comparing with planktonic cells, while ATCC 90038 didn't.</p><p><b>CONCLUSION</b>The members of ALS gene family may play important roles in the course of Candida albicans biofilm formation.</p>