ABSTRACT
Non-syndromic cleft lip with/without cleft palate (NSCL/P) is a common congenital disease worldwide, and its etiology is related to the combination of genetic and environmental factors. Although genome-wide association analysis did not involve Single Nucleotide Polymorphisms (SNPs) in Wnt genes, it has been reported that SNPs in Wnt genes are related to NSCL/P, and the study of SNPs of Wnt genes and its corresponding phenotypic effect is helpful to explain the etiological mechanism of NSCL/P. In recent years, Wnt3 gene related to NSCL/P, rs142167, rs3809857, rs9890413 and other teratopoietic single nucleotide loci are the main teratopoietic single nucleotide loci studied most, and the research conclusions on the correlation between Wnt3 gene polymorphism and NSCL/P are obviously different in different populations. This paper reviews the research progress on the correlation between Wnt3 gene polymorphism and NSCL/P.
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of oral mucosa cell transfected with IFN-gamma recover denuded hard palate to the growth of maxilla in rats.</p><p><b>METHODS</b>Divide 3-week-age 80 female rats with left denuded hard palate into 4 groups randomly, depends on different methods recovered the denuded hard palate, granulation tissue growing itselves (I), transplanting amnion (II), transplanting oral mucous cell loaded by amnion (III), or oral mucous cell transfected with IFN-gamma (IV), 20 rats in one group. After 9 weeks, measure the width of left hard palate and the right, and analyse the width of hard palate by different methods.</p><p><b>RESULTS</b>The antisymmetry rate of hard palate width of groups I, II, III and IV are (68.64 +/- 9.03)%, (58.53 +/- 7.40)%, (53.12 +/- 4.92)% and (52.25 +/- 4.61)%, statistical analysis through SNK of SPSS 11.0 show group I is different from groups II, III, IV (P < 0.05), group II is different from groups III, IV (P < 0.05), but group III is not different from group IV (P > 0.05).</p><p><b>CONCLUSIONS</b>It has energetic significance to prevent maxillary secondary deformity with amnion and tissue-engineered oral mucosa recover denuded hard palate, the tissue-engineered oral mucosa is better than amnion, the tissue-engineered oral mucosa transfected with IFN-gamma is better than not transfected.</p>
Subject(s)
Animals , Female , Rats , Cleft Palate , General Surgery , Interferon-gamma , Genetics , Maxilla , Congenital Abnormalities , General Surgery , Maxillofacial Development , Mouth Mucosa , Transplantation , Oral Surgical Procedures , Palate, Hard , Rats, Sprague-Dawley , Stem Cells , Cell Biology , Surgical Flaps , Tissue Engineering , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To confirm if denuded bone on lateral aspects of the hard palate of surgical repairs of the cleft palate is the main reason of causing disturbance of facial growth and to investigate the mechanism.</p><p><b>METHODS</b>48 SD rats were employed in the study. Among them 40 animals were divided into normal control and experimental group randomly. Normal control was not operated. Hard palate mucoperiosteum on the left side in experimental group were excised. Left hard palate mucoperiosteum in another 8 female three-week-old SD rats were excised with same method and were sacrificed at 2nd, 5th, 8th, and 12th day, respectively. The 8 rats were used for histological research.</p><p><b>RESULTS</b>The width of all left sides hard palates in experimental group was significantly narrower than right side, and demonstrated statistical difference. Chronic osteomyelitis on the denuded hard palate was observed.</p><p><b>CONCLUSION</b>The denuded bone wound might be a principal factor for the following maxillary growth deformity in cleft palate patients who received palatoplasty in childhood. The mechanism of causing the deformity might be wound and scar contraction, decreased blood flow and nutrition plus chronic osteomyelitis.</p>