ABSTRACT
Neurological diseases include a variety of neurodegenerative diseases and other brain damage diseases.The treatment schemes for neurological diseases are still in research.The existing clinical and basic studies have confirmed that traditional estrogen therapy has certain protective effect on the nervous system,while it increases the risk of breast or endometrial cancer.The emergence of the selective estrogen receptor modulators (SERMs) can avoid the above mentioned problems.The available studies have confirmed the protective effect of tamoxifen as a SERM on the nervous system.This paper reviews the role and functioning mechanisms of tamoxifen in the nervous system and cognitive function,aiming to provide guidance for the future application of tamoxifen in the treatment of neurological diseases and the improvement of cognitive function.
Subject(s)
Tamoxifen/therapeutic use , Selective Estrogen Receptor Modulators/therapeutic use , Cognition , Nervous SystemABSTRACT
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.
Subject(s)
Bacteremia , Diagnosis , Genetics , Bacterial Typing Techniques , Bacteriological Techniques , DNA Probes , Genetic Techniques , Listeria monocytogenes , Metabolism , Nucleic Acid Hybridization , Oligonucleotide Array Sequence Analysis , Oligonucleotides , Chemistry , RNA, Ribosomal , Chemistry , RNA, Ribosomal, 23S , Genetics , Stem CellsABSTRACT
<p><b>OBJECTIVE</b>To differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrB gene sequences.</p><p><b>METHODS</b>GyrB sequences of 19 strains of E.coli, 13 Shigella spp. 2 Aeromonas caviae, 2 Aeromonas hydrophilia,1 Aeromonas veronii were determined and combined with sequences retrieved from public databases to construct phylogenetic trees. For each sequence tested, the identification deduced from the clustering relation of sequences was compared with the phenetic identification.</p><p><b>RESULTS</b>All the tested sequences, except those of Shigella boydii and Shigella dysenteriae, were corresponded with the 5 closest sequences on the tree at the species level. While the BLAST queries returned some other bacteria organisms or undetermined entries.</p><p><b>CONCLUSION</b>Phylogenetics displays good discriminative power in bacterial sequences differentiation.</p>