ABSTRACT
Objective To investigate the clinical significance of the level of regulatory T(Treg)cells and indoleamine 2,3-dioxygenase(IDO)in HBV-induced hepatocellar cancinoma.Methods 51 cases of HBV-DNA positive primary liver cancer(HCC),44 cases of chronic hepatitis B(CHB),29 cases of cirrhosis and 28 cases of healthy volunteers(control group)were used RT-PCR and flow cytometry to detect the expression of Treg cells and IDO in peripheral blood of each group.Results The expression of IDO mRNA from high to low were HCC group,CHB group,liver cirrhosis group and control group.The control group and the liver cirrho-sis group were lower than the other 2 groups(P<0.01),and the difference between the other groups was sta-tistically significant(P<0.05).Treg counts from high to low were HCC group,cirrhosis group,CHB group and control group.HCC group was significantly higher than that of control group(P<0.05),except for CHB group,Treg cells in each group were statistically significant compared with control group(P<0.05).The cor-relation analysis of expression of IDO mRNA and Treg:r=0.912,P=0.000,indicating that the expression of IDO in peripheral blood was positively correlated with the Treg cells.Conclusion IDO is over expressed in HCC with the high Treg cell level.Treg cells and IDO may associated with hepatocellular carcinoma immune escape mechanisms.
ABSTRACT
Objective To investigate prolactin(PRL)levels in patient with hyperprolactinemia in different period of time. Methods Used electrochemical luminescence method,Roche E170 automatic immune analyzer determination of detect 124 cases female patients of hyperprolactinemia (PRL>880 uIU/ml)at 7:30AM,10:00AM and 4:00PM respectivelye of plas-ma PRL level,and in accordance with the polyethylene glycol (PEG)6000 precipitate PRL after A PRL recovery rate of into macroprolactin (MPRL)group (A PRL recovery rate of≤40%),single PRL group (A PRL recovery rate of> 60%),sus-picious PRL group (40%