ABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents from Centella asiatica.</p><p><b>METHOD</b>Chemical constituents were isolated by repeated column chromatography (Toyopearl HW-40C and HPLC) and their structures were elucidated on the basis of spectroscopic method.</p><p><b>RESULT</b>Five compounds were identified as: docosyl ferulates (1), bayogenin (2), 3beta-6beta-23-trihydroxyolean-12-en-28-oic acid (3), 3beta-6beta-23-trihydroxyurs-12-en-28-oic acid (4), D-gulonic acid (5).</p><p><b>CONCLUSION</b>All of the Compounds were isolated for the first time from C. asiatica.</p>
Subject(s)
Centella , Chemistry , Chromatography, High Pressure Liquid , Methods , Coumaric Acids , Chemistry , Molecular Structure , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>A HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.</p><p><b>METHOD</b>The column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).</p><p><b>RESULT</b>The linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).</p><p><b>CONCLUSION</b>The method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.</p>