[Toxicity Evaluation of coral madrepora and human BMG in peripheral mononuclear blood cells]

Disadvantage of auto grafts make widespread use of other bone graft materials such as xenografts and allografts. cytotoxic effect of materials and biomaterials on Lively human cells is the Object that always should be observed. In present study had tried to comparison evaluation cytotoxic effete of two bio material -human BMG and coral madrepora -on human peripheral mono nuclear blood cells. In present experimental study after to make preparation peripheral blood with fycole method and After preparation and sterilization of human BMG and coral madrepora, Then, human BMG and coral madrepora, aside to gether are divided into 10, 20, 50 mg portions and cultured on 24-well of 100000 mononuclear cells of peripheral blood and they are evaluated after 24, 48 and 72 hours. MTT method was used for evaluation of cytotoxic effect using ELISA reader in 540 nm wave lengths. Light absorption rate depends on MTT uptake by the cells, indicating vital activity of the cells. Result evaluated with variance and whilcoxon-rank sum and mann u whitnney test. All of the human BMG doses that were used weren,t toxic and all of them increased number of the human peripheral blood mononuclear cells [P< 0.005]. But coral madrepora, was decried number of the human peripheral blood mononuclear cells after 24 hour in 50 mg doses and after 48 hours in 10 mg doses and after 72 hours in 20 and 50 mg doses. With increasing of human BMG concentration and passing time, increasing of human peripheral blood mononuclear cells can be observed and it has a statistically significant difference with control group. Therefore, as a conclusion, human BMG is compatible with human blood mononuclear cells. But coral madrepora had toxicity for this blood cells. However for more judgment, need more study with different cells

[Histomorphometric comparison of vertical bone augmentation between autogenous demineralized dentin matrix and biooss on parietal bone of rabbit]

Lack of sufficient bone is a main problem in implant treatment. Bone graft materials are potential solutions to this problem. Bioose is a grafting material that is routinely used for this purpose. In some studies autogenous demineralized dentin matrix [ADDM] has been used as bone substitute. The aim of this investigation was to compare the two grafting materials [ADDM and Biooss] on vertical bone augmentation in parietal bone of rabbits. Six white Newsland rabbit were used in this study. Two screws were inserted on the parietal bone of each rabbit in anteroposterior direction. Biooss powder was put around the anterior screw and ADDM particles around the posterior screw and both were covered with gutta percha membrane. The rabbits were sacrificed after 12 weeks and the specimens were sent for histological examination. Mann Whitney and paired T-tests were used to analyze the data. Range of bone height in Biooss group were between 0.1-2.1 and in ADDM group were 0.4-3.2 mm. Mean Bone height in Biooss group was 1.43 +/- 0.84 and in ADDM group was 1.4 +/- 1.03. Mann Whitney test showed no significant difference between the two groups in bone height. In this study the vertical bone augmentation in Biooss and ADDM groups were the same. Therefore, ADDM was an effective biomaterial for vertical bone augmentation in this study

[Evaluation of coral [madrepora] cytotoxicity in fibroblast cell culture]

Bone defect is one of the major problems facing dentists and maxillofacial surgeons. Several approaches, such as incorporating autografts, xenografts, as well as using polymers have been suggested. The calcified skeleton of corals has been proposed for bone grafting over the past few years. Toxicity is the most important concern of grafting foreign material. The purpose of this study was to investigate the toxicity of coral [madrepora] skeleton in fibroblast culture. Powdered corals with particle sizes of less than 100 micron were autoclaved. Coral powder was added to culture medium consisting of 90% RPMI, 10% FBS and different concentrations of 0.5, 1, 2, 5, 10, 20 and 50 milligrams per 100 milliliters of media were prepared. Fibroblast cells were obtained from Pasteur institute for inserting into culture mediums. Three samples were prepared from each concentration. Complete medium culture was the control. Ninety six well plate containing culture medium, fibroblasts and coral powder were prepared. They were placed into a programmed incubator containing 5% CO2 for 24, 48 and 72 hours at 37°C. The [MTT] was then added to plate culture mediums. Since light absorption has close relationship with cells viability, ELISA reader apparatus was used to measure light absorption [optic dosimeter]. The collected data was statistically analyzed using ANOVA test. Light absorption in control group was similar to light absorption in the various concentrations of coral medium culture along with incorporated fibroblasts. Based on the findings of this study, it can be concluded that coral [madrepora] has potentially no cytotoxic effect on fibroblast cells

histological study of the efficacy of the madrepora particles on parietal bone healing of rabbit

G.B.R is a procedure which is used for treatment of the osseous defects. In this procedure new bone forms in the defect. The aim of this study was to compare the bone formation with madrepora and membrane, madrepora alone and membrane alone. This study was done on 5 white Newzl and rabbits. Three defects were created in parietal bone. The first defect was filled with madrepora particles [250-500 micron] and covered with a piece of membrane. The second defect was filled only with madrepora and the third defect was left empty and covered with membrane. After 8, 9, 11, 14, and 15 weeks animals were sacrificed. Statistical analyses were done by Kruskal - wallis and Mann -whitney U tests. Histologically the defect which contained madrepora and membrane was filled with trabecules after 15 weeks. In the second defect the trabecules were seen only in the periphery. The defect was filled with connective tissue in the third defect. Madrepora's pieces have biocompability with rabbit tissues and the process of repair in the osseous defect was accelerated

Effect of glucose - insulin - potassium [GIK] solution on short term prognosis of unstable angina

Unstable angina as a clinical condition includes a major group of patients manifested with acute coronary syndrome. Misdiagnosis of this clinical syndrome causes myocardial infarction [MI] and death. Conventional and advanced forms of treatment are used with the aim of rapid stabilization of unstable angina. Although infusion of glucose - insulin - potassium [GIK] solution has had good results in acute MI, no major trial has studied its effect in unstable angina. The main goal of this study was evaluation of the effectiveness of GIK solution on prognosis of hospitalized unstable angina patients. This randomized clinical trial included patients with class II and III unstable angina [two groups of 94 patients, each] with a mean age of 62.47 +/- 13.20 years and Female /Male ratio of 1.35 admitted in the CCU's of Yazd from September 2003 to May 2004. There was no significant difference between the study and control groups regarding mean age, sex ratio and unstable angina class [P = 0.15, P = 0.77 and P = 0.76]. The study group had significant reduction in recurrent chest pain and duration of hospitalization [P = 0.001 and P = 0.02]. The most common adverse effect ofGIK solution was pain at infusion site. Use of GIK solution causes early stabilization of unstable angina patients without any significant or life threatening adverse effect

[Comparison of hospital based cancer registry of Iran and the United States of America]

Cancer research is one of the essential activities for its control and treatment. Hospital based cancer registry system is an information system designed to collect, organize and analyze data on cancer. The objective of the present study was to compare hospital based cancer registry system in Iran with that in the USA. This research was a comparative study. Studied population included all the health care centers of Tehran, Shaheed Beheshti and Iran medical sciences universities having a pathology laboratory. The findings indicate that [Pars] software program is mainly used in pathology, while [ADS-9] program in medical record departments of the hospitals .Furthermore, requested information about cancer cases was mostly by researchers and students and the least by the hospital heads, cancer committees and physicians councils. 4-8% of cancer information from the pathology departments was demanded by hospital managers, pathology committees, physicians, researchers and students. 60% of cancer information from [medical record departments] was reported to the drug and care deputy of the medical sciences universities to which the hospitals were affiliated. 56% of cancer information from the pathology departments was reported to the health centers outside the hospitals. The hospital based cancer registry system in Iran is imperfect and limited in scope as compared to the registry system in the U.S. The cancer registry in Iran only records demographic information of patients and the type of tumors. Learning from the hospital based cancer registry of the U.S.A can help to improve the hospital cancer registry based system in Iran