ABSTRACT
Background: Aim of the study was to study the age, incidence, occupational factor, history of presenting illness, hospital stay before and after surgery, various types of surgical approaches and their outcome in the form of cosmetic outcome and recurrence rate in 20 cases of pilonidal sinus done in 2012-13 in SIMS - Shivamogga Institute of Medical Sciences. Methods: Primary closure, healing by secondary contracture. Results: Out of the 20 cases operated for pilonidal sinus, 12 were male and 8 were female out of which 5 belonged to 10-20 year age group; 12 belonged to the 20-30 year age group; while 3 belonged to 30-40 year age group. Conclusion: Most common age group involved is 20-30 years, suggesting it is more common in physically active age group and since most of them are manual laborers it doesn’t show any preference to sedentary workers or those who sit for long hours. Also it is more common in males than in females.
ABSTRACT
Diabetes is characterized by derangements in carbohydrates, protein and fat metabolism caused by the complete or relative insufficiency of insulin secretion. The decreased oxidative stress in diabetes includes the autoxidation of glucose and non enzymaticglycation and also changes in the antioxidants defense systems. As the diabetes is lowered in human the pharmacological effects of a drug becomes toxic. Our aim was to evaluate the invivo effect of the anti diabetic compound Vanadium in non- diabetic rat models. The oral administration of Vanadium peroxide shows significant changes in the Cholesterol and TG with organ morphology and enhancement in clinical and experimental parameters.Vanadium is an important environmental and industrial pollutant. It is a dietary micronutrient and it has been recently considered as a pharmacological agent. Vanadium compounds have a major role in cellular regulation, with profound effects on enzymes of plasma membrane. Vanadium is one of the abundance trace elements widely distributed in the environment. It is used in steel and chemical industries, and is a containment of many ores, coals, and petroleum oils the manufacture of pigments, in photography and in insecticides. It is been used medicinally as anticeptic, spirochetocide, antituberculotic and antianemic agents and general tonic.
ABSTRACT
Andrographis paniculata (Acanthaceae) has been used to treat hepatoprotective, antidiarrhoeal, antiinflammatory, antimalarial, and antihypertensive. The aim of the study was to investigate the antifungal activity of different part of extracts of A. paniculata. The antifungal activity of extracts of A. paniculata was evaluated by Agar well diffusion method against five selected fungal species. Stem extracts of A. paniculata showed high antifungal activity against A.oryzae, Penicillum sp and C.albicans. The root extracts showed high antifungal activity against A. niger, A. flavus, C.albicans, Penicillum sp and A.oryzae and also leaf extracts showed high antifungal activity against Penicillum sp and A. flavus but did not show antifungal activity against C.albicans, A. niger, A.oryzae. The results obtained in the present study suggest that A. paniculata plant can be used in treating various diseases caused by the test organisms.
ABSTRACT
CREB binding protein (CBP) and E1A binding protein p300, also known as p300 are functionally related transcriptional co-activators (CoAs) and histone acetyltransferases (HATs). Some small molecules, which target HATs can activate or inhibit the p300 enzyme potently. Here, we report the binding affinities of two small molecules CTPB [N-(4-chloro- 3-trifluoromethyl-phenyl)-2-ethoxy-6-pentadecyl-benzamide] and CTB [N-(4-chloro-3-trifluoromethyl-phenyl)-2-ethoxy-benzamide] with p300 using docking method to obtain the insight of their interaction with p300. These small molecules bind to the enzyme, subsequently causing a structural change in the enzyme, which is responsible for the HAT activation. CTB exhibits higher binding affinity than CTPB, and their lowest docked energies are -7.72, -1.18 kcal/mol, respectively. In CTPB molecule, phenolic hydroxyl of Tyr1397 interacts with the non-polar atoms C(5E) and C(5F), and forms polar-non polar interactions. Similar interactions have also been observed in CTB. The residues Tyr1446 and Cys1438 interact with the non-pentadecyl atoms. Further, the docking study predicts a N-HO hydrogen bonding interaction between CTB and Leu1398, in which the HO contact distance is 2.06 Å. The long pentadecyl chain of CTPB reduces the formation of hydrogen bond with the p300. The H-bond interaction could be the key factor for the better activation of CTB.