ABSTRACT
The present study was to extract the mucilage from the Okra plant (Abelmoschus esculentus) and to study the effect of mucilage concentration on in vitro release of Lamivudine from it’s sustained release matrix tablets. Mucilage was extracted from the fruits of Abelmoschus esclentus using organic solvent Acetone. The extracted mucilage was subjected to various physiological properties for its suitability as an excipient in the preparation of tablet. Lamivudine sustained release tablets were prepared using different concentration of Okra mucilage as a sustained release matrix excipient. The formulated tablets were evaluated for post compression parameters such as weight variation, hardness, friability, wetting time, water absorption ratio, and in vitro drug release studies. Stability studies of optimized formulation were carried out for three months. The results of in vitro release revealed that the release rate decreased with increase in the concentration of mucilage. The release kinetics indicated that the nature of drug release from the matrix tablets was dependent on drug diffusion and polymer relaxation and therefore followed non-fickian or anomalous release. No incompatibility was observed between the drug and excipients used in the formulation of matrix tablets. The Okra mucilage showed promising results in terms of sustaining the release behavior of Lamivudine from the matrix. The developed sustained release tablets of Lamivudine, with extension of release up to 12 hours, can overcome all the disadvantages of conventional Lamivudine tablets.
ABSTRACT
The aim of the study was to investigate the hepatoprotective and antioxidant potential of methanolic extract of Polyalthiya longifolia fruits (MEPL) by in-vitro and in-vivo methods.In the invitro study, Freshly isolated rat primary hepatocytes and HepG2 cells were exposed with CCl4 along with/without various concentrations of MEPL(125,250,500 μg/kg) and the effects were studied.In the in-vivo studies, CCl4 intoxication method was used and aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin and total proteins were estimated and supported with histopathological studies.MEPL (125,250,500 μg/kg) treated animals increased the percentage of viability in both primary hepatocytes (p<0.001) and in HepG2 cells (p<0.01) where as in the invivo studies,MEPL produced significant hepatoprotective effect by decreasing the elevated serum enzymes, bilirubin, LPO and significantly increased the levels of glutathione (GSH), catalase (CAT) and super oxide dismutase (SOD) .Moreover, in-vitro antioxidant studies revealed that MEPL scavenged free radicals and maximum percentage of inhibition was 62% at 800μg/mL. In addition to this, treatment with MEPL fruits showed a dose dependent reversal of histopathological changes. Based on the results, we conclude that, MEPL possesses hepatoprotective and antioxidant activity against CCl4 intoxication in both in-vitro and in-vivo methods through its free radical scavenging and antioxidant properties.