[Efficiency improvement of fluticasone propionate dry powder formulation for inhalation in pulmonary patients]

Background: Nowadays, inhaled corticosteroids have become the core of therapy for patients with asthma and chronic obstructive pulmonary disease [COPD]. Fluticasone propionate [FP] is among the most lipophilic steroids [water solubility 0.1 mg/ml] and its clinical efficacy has been confirmed in many studies .The inclusion of different carrier materials in a dry powder inhaler [DPI] system can alter formulation performance, which might be attributable to variation in the adhesion between drug and carrier particles.

Materials and methods: To this aim, we used two different carriers to be mixed in different times with drug on the one hand to facilitate the powder flow during manufacturing, and on the other hand to help fluidization upon patient inhalation. The prepared DPI formulations were examined in terms of drug content uniformity upon the physical blending, particle shape, and in vitro pulmonary deposition. The latter was determined using an Andersen cascade impactor and Cyclohaler® as a single-dose DPI

Results: Our results revealed that the highest fine particle fraction [FPF] and fine particle dose [FPD] value belonged to the formulation contained FP: mannitol, mixed in 30 minutes

Conclusion: It can be concluded that using mannitol as the coarse carrier can improve the physical properties as well as the aerosolization behavior of the resultant DPI formulations

novel high performance liquid chromatographic method for determination of nystatin in pharmaceutical formulations by box–behnken statistical experiment design

In this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box-Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column [30 [degree]C] using a mobile phase consisting of ammonium acetate 0.05 M buffer/ Methanol mixture [30:70] and a flow-rate of 1.0 mL/min. The specificity, linearity, precision, accuracy, LOD and LOQ of the method were validated. The method was linear over the range of 5-500 microg/mL with an acceptable correlation coefficient [r[2] = 0.9996]. The method's limit of detection [LOD] and quantification [LOQ] were 0.01 and 0.025 microg/mL respectively. The results indicate that this validated method can be used as an alternative method for assay of nystatin

[Evaluation of efficiency of some disinfectants in disinfection of water treatment system in the pharmaceutical industry and select the best one]

Water used in the pharmaceutical industry should have special physicochemical and microbiological characteristics. One of the quality items of pharmaceutical water is level of microbial content. For this reason selection of effective disinfection method is the main item for design of pharmaceutical water systems. Water systems can be maintained at temperature below 10 [degree sign]C and more than 70 [degree sign]C but maintaining of water at ambient temperature could increase of microbial content. In this condition use of disinfectant is useful for reducing of microbial content. Some of them such as chloride, ozone and hydrogen peroxide due to good results have more application. In this study, effect of three disinfectants chloride, ozone and hydrogen peroxide with 1 ppm concentration, consider on five microorganisms. Pseudomonas aeruginosa, Staphylococcus aureus, candida albicans, Aspergillus niger and E.coli. Counting and decreasing of microorganisms showed the efficiency of disinfectants. Finally ozone was selected as the best disinfectant due to high efficiency