ABSTRACT
Background: Lichen planus (LP) is an idiopathic, chronic, relapsing, inflammatory, autoimmune dermatological disease. The etiopathogenesis of LP is still unclear. Autophagy is a strictly regulated lysosomal degradation pathway that is crucial for maintaining intracellular homeostasis and normal development. The dysregulation of autophagy-associated genes was recognized to increase the susceptibility to multiple diseases, including inflammation, autoimmune disorders and cancer. Aims: Our study aimed to detect the expression of autophagy-related gene 9 b (ATG9B) in LP patients compared to normal control persons to investigate the possible role of autophagy in pathogenesis of this disease. Methods: This case–control study included 30 LP patients and 30 age-, gender-matched healthy controls. Four millimeters punch skin biopsies were obtained from LP lesions and from the controls and they were kept in lysis solution for the stability of the studied parameters and were kept frozen at –80°C till analysis of ATG9B using real-time polymerase chain reaction. Results: The level of ATG9B in lesional skin of LP was significantly decreased compared to normal control persons (P < 0.01); also, there was a non-significant relation between ATG9B level and age, sex, duration and family history among LP patients. Limitations: Limited number of patients included in our study (30 patients). Conclusion: Autophagy may play a role in the pathogenesis of cutaneous LP.
ABSTRACT
Background: Systemic lupus erythematosus [SLE] is a systemic autoimmune disease characterized by various immunological abnormalities, including dis-regulating activation B lymphocytes with subsequent productionof a large quantity of autoreactive-antibodies. It is also hypothesized that T helper-17 lymphocytes [TH-17] may have a role in this disease. The aim of the present work was to determine the role of TH-17cells expressing the retinoid acid related orphan receptor gamma t [ROR-gamma-t] mRNA in the pathogenesis of SLE disease
Methodology: The study was conducted on 30 female SLE patients fulfilling SLICCA /ACR criteria for SLE classification and 30 healthy subjects sex- and age-matched apparently as control group with no previous history of autoimmune diseases. SLE Disease Activity Index was calculated for SLE patients. Level of expression of [ROR-gamma-t] mRNA of IL-17 were measured in all patients and control by quantitative Real Time Polymerase chain reaction [Q PCR]
Results: The mean +/- SD of ROR-gamma-t mRNA expression levels in SLE patients [3.6+/-6.1] was significantly reduced compared to that of controls [11.7+/-13.7] [p= 0.008].Neither the clinical features of SLE nor the laboratory parameters have significant relationship with ROR-gamma-t expression
Conclusion: The reduction of ROR-gamma-t mRNA expression in TH-17 lymphocytes may point out to the regulatory protective role of TH-17 in the pathogenesis of SLE. Agents that block the functions of these cells should be tried
ABSTRACT
The primary medical report is a valuable tool for retrieving any information concerning the patient's medical condition. This study was conducted to evaluate the form and contents of the primary medical reports for traumatic cases at the Emergency Department at Suez Canal University Hospital. This is a descriptive comparative study in which 212 reports were included, divided into 2 groups: prospective group [A] and retrospective group [B] and were evaluated according to: administrative data, ethical data, medical data, wound description, and medico legal data. The results recorded that there is a significant decrease [P<0.05] in reporting the name of the report's recipient with a clear signature, and in reporting the presence of other injuries and the probability of complications in group A, while vital signs, investigations and management were not recorded in both groups. Dimensions of wounds were significantly decreased [P<0.05] in group A, while the precise description of the site was significantly increased [P<0.05] in the same group. Edges and vital reactions were not recorded in both groups. The type and age of wounds and comment on clothes were not reported in both groups. The duration of healing was significantly decreased [P<0.05] in group A but it was reported correctly. The study demonstrates the lack of accurate issuing of the primary medical reports for traumatic cases at Suez Canal University Hospital. The study has provided a recommended form of the primary medical report designed by the researcher, diagrams demonstrating anatomical body regions in Arabic language and Arabic translation of some important terms used in traumatic medical reports