Application of artificial neural network algorithm in pathological diagnosis and prognosis prediction of digestive tract malignant tumors / 浙江大学学报·医学版

The application of artificial neural network algorithm in pathological diagnosis of gastrointestinal malignant tumors has become a research hotspot. In the previous studies, the algorithm research mainly focused on the model development based on convolutional neural networks, while only a few studies used the combination of convolutional neural networks and recurrent neural networks. The research contents included classical histopathological diagnosis and molecular typing of malignant tumors, and the prediction of patient prognosis by utilizing artificial neural networks. This article reviews the research progress on artificial neural network algorithm in the pathological diagnosis and prognosis prediction of digestive tract malignant tumors.

Balance and Stability for Patients with Idiopathic Normal Pressure Hydrocephalus / 中国康复理论与实践

@#Objective:To observe the features of posture control in patients with idiopathic normal pressure hydrocephalus (iNPH). Methods:From May, 2017 to May, 2018, patients with iNPH in our hospital (<italic>n</italic> = 13) and healthy controls (<italic>n</italic> = 15) were measured with sensory organization test on Balance Manager. The balance scores and strategy scores under six conditions were recorded and the comprehensive balance scores were calculated. Results:The balance scores decreased in the patients in the conditions of eyes-open/stable-support, eyes-close/stable-support, eyes-close/unstable-support and eyes-disturb/unstable-support compared with those of the controls (|<italic>Z</italic>| > 2.042, <italic>P</italic> < 0.05), as well as the comprehensive balance scores (<italic>Z</italic> = -3.617, <italic>P</italic> < 0.001); while the strategy scores increased in the conditions of eyes-close/stable-support, eyes-open/unstable-support, eyes-close/unstable-support and eyes-disturb/unstable-support (|<italic>Z</italic>| > 2.333, <italic>P</italic> < 0.05). Conclusion:There are balance disorders in the patients with iNPH, mainly associating with the disorders of proprioceptive and vestibular senses, and application of hip strategy.

Application of Pneumatic Artificial Muscle in Rehabilitation Equipment (review) / 中国康复理论与实践

This paper introduced the application of pneumatic artificial muscles in rehabilitation medical equipment at home and abroad, and emphatically analyzed the application of pneumatic artificial muscle in upper limb rehabilitation instruments and lower limb rehabilitation instruments in recent years, among which upper limb rehabilitation instruments included hand, wrist and arm, and lower limb rehabilitation instruments included leg, knee and ankle. The working characteristics and application scope of the application examples were introduced, and a comparative analysis of the same functional rehabilitation equipment was made.

Design of Spatial Trajectory Crawl Training Device / 中国康复理论与实践

Objective To design a space crawl training device.This device could achieve oval movement by rotating the wheel to drive the handle of the upper limb.At the same time,the leg support is driven to rotate about 30 degrees to 0 de-grees,which can assist the user to complete the entire crawling. Methods The structure of the device was modeled using SolidWorks.Then the whole device was simulated by using SolidWorks Motion,and the dynamic analysis was completed by calculation.Finally,the main force components from SolidWorks were imported into ANSYS Workbench for static analysis. Results and Conclusion The designed space trajectory crawling training device can assist the user to complete the scheduled crawling movement,and the strength checking of the key components meets the strength requirements.

Structure design and calculation analysis for double gasbags based on body weight support treadmill training / 医用生物力学

Objective To develop a double-gasbag weight loss equipment based on body weight support treadmill training (BWSTT)，and analyze the reasonability of the structure design. Methods The mathematical model of double gasbags in the process of air inflation was established, and the 3D model of double gasbags was established and meshed using Gambit; the analog computation for gas flow of the gasbag was conducted using Fluent; normal subjects with body weight below 80 kg were selected for clinical experiments based on designing and roughing of the prototype. Results The inflation time of double gasbags was 16.9 s and the maximum weight loss percentage was 90%; the pressure inside double gasbags was 2 kPa, and the relationship between the weight loss percentage and inside pressure of the gasbag was obtained by clinical experiments, which verified the reasonability of the gasbag structure. Conclusions The double-gasbag structure can meet the requirements of weigh loss training, which provides an effective weight loss support method and a feasible structural design for patients with lower limb dysfunction during rehabilitation training.

Development of external therapeutical ultrasound systems (ETUS) for acute myocardial infarction / 中国医疗器械杂志

This paper introduces the principle and structure of the ETUS system for acute myocardial infarction. This system is an assistant treatment tool with ultrasound waves acting from the external on the heart, speeding thrombolytic drug's permeation into the thrombi for a good curative effect.

Pharmacokinetics of oxymatrine and its metabolite in beagle dogs by LC-MS / 中国中药杂志

<p><b>OBJECTIVE</b>To establish LC-MS method in the determination of oxymatrine and its metabolite in plasma and investigate their pharmacokinetics in beagle dogs.</p><p><b>METHOD</b>Lichrospher C18 column (4.6 mm x 250 mm, 5 microm) was used as the analytical column maintained at 25 degrees C. The mobile phase consisted of 10 mmol x L(-1) CH3COONH4 and CH3OH (25:75). Flow rate was 1 mL x min(-1). Electrospray ionization (ESI) was carried out. The ESI ion source was set in positive ion polasity mode. The selective ion monitoring (SIM) was set at m/z 265.1 and 249.2.</p><p><b>RESULT</b>The linearity ranged from 2 to 5000 ng x mL(-1) (r = 0.9991). The detection of oxymatrine and its metabolite were 0.6 and 0.3 ng x mL(-1). The RSD(%) within day and between day was less than 4.7%. The recovery of this method was more than 96.5%. The disposition was conformed to a two-compartment model. The T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of oxymatrine were (5.5+/-1.58) h, (1.0+/-0.30) h, (2418.3 +/-970.78) ng x mL(-1), (3.2+/-0.64) h, (5797.4+/-908.16) ng x mL(-1) x h accordingly. The corresponding T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of matrine were (9.8+/-2.77) h, (1.9+/-1.09) h, (1532.4+/-494.86) ng x mL(-1), (4.4+/-1.97) h, (5530.5+/-1042.65) ng x mL(-1) x h.</p><p><b>CONCLUSION</b>This assay was highly sensitive, rapid, simple and specific enough for determining concentrations of oxymatrine and its metabolite matrine in plasma of beagle dog.</p>

Determination of first-order structure of somatostatin by electrospray ionization mass spectrometry / 药学学报

<p><b>AIM</b>To determine the molecular weight and first-order structure of somatostatin.</p><p><b>METHODS</b>The molecular weight of somatostatin was determined by electrospray ionization mass spectrometry. Somatostatin was deoxidized by 2-mercaptoethanol. A series of typical fragment ions of deoxidized product were obtained by insource collision-induced dissociation (CID).</p><p><b>RESULTS</b>The m/z of quasi-molecular ion [M + H]+ of somatostatin was 1,637.8 and [M + Na]+ was 1,659.5. The m/z of double-charge ion [M + 2H]2+ was 819.5 and [M + H + Na]2+ was 830.3. It showed that the molecular weight of somatostatin was 1,636.7. The y and b series of fragment ions of deoxidized product were obtained by adjusting the fragmentor voltage. It was determined that the first-order structure of deoxidized product of somatostatin was A-G-C-K-N-F-F-W-K-T-F-T-S-C.</p><p><b>CONCLUSION</b>The molecular weight and first-order structure of somatostatin were confirmed.</p>

Analysis of impurity in caderofloxacin / 药学学报

<p><b>AIM</b>To analyse the main impurity of caderofloxacin.</p><p><b>METHODS</b>The impurity of caderofloxacin was analysed and determinated by RP-HPLC/ESI/MS with a Zorbax SB-C18 (150 mm x 4.6 mm ID, 5 microns) column. The mobile phase was acetonitrile-0.5% acetic acid solution (17:83). A compound was synthesized: 1-cyclopropyl-8-(difluoromethoxy)-6-fluoro-1, 4-dihydro-7-(1-piperazinyl)-4-oxo-3-quinoline carboxylic acid (DMCA). Its HPLC chromatogram, UV and MS spectrum were compared with those of the impurity in caderofloxacin.</p><p><b>RESULTS</b>The molecular weight of the impurity was 14 less than that of caderofloxacin. It means the impurity was a CH2-group less than caderoflixacin. The tR, UV and MS of DMCA were the same as those of the impurity in caderofloxacin.</p><p><b>CONCLUSION</b>Based on the tR (HPLC), UV and MS, the impurity of caderofloxacin is confirmed as DMCA.</p>

Study on the fingerprints of the alkaloids in Sophora flavescens / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a method for HPLC fingerprint determination of the alkaloids in S. flavescens.</p><p><b>METHOD</b>RP-HPLC, linear gradient elution, LC/MS, etc. were used to determine the fingerprint and identify the main peaks in the HPLC fingerprint.</p><p><b>RESULT</b>A satisfactory method for HPLC fingerprint determination of the alkaloids in S. flavescens. was established, and 5 peaks in the HPLC fingerprint were identified.</p><p><b>CONCLUSION</b>The perfect fingerprint can be obtained and the method can be used for quality control of S. flavescens.</p>