ABSTRACT
Abstract Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida.
Subject(s)
Animals , Drug Resistance, Bacterial , Pasteurella Infections/veterinary , Pasteurella multocida/drug effects , Pasteurella multocida/pathogenicity , Poultry Diseases/microbiology , Swine Diseases/microbiology , Virulence Factors/analysis , DNA, Bacterial/genetics , Genotype , Microbial Sensitivity Tests , Polymerase Chain Reaction , Poultry , Pasteurella Infections/microbiology , Pasteurella multocida/isolation & purification , Serotyping , Swine , Virulence Factors/geneticsABSTRACT
Eighty Salmonella Enteritidis strains isolated from broiler carcasses between May 1995 and April 1996 in the State of Rio Grande do Sul, Brazil, were tested for antibiotic susceptibility using the disk diffusion method. Resistance to colistin, novobiocin, erythromycin and tetracycline was observed in 100 percent of the isolates. The strains showed intermediate resistance at different levels to kanamycin (1.25 percent), enrofloxacin (3.75 percent), neomycin (3.75 percent), fosfomycin (20 percent), sulphonamides (86.25 percent) and nitrofurantoin (90 percent). Resistance to ciprofloxacin, norfloxacin, gentamicin, polymyxin B, sulphametrim and sulphazotrim was not found. Since resistance to antibiotics especially those introduced in the last decades, was detected, it is recommended that their use must be based on the results of resistance tests or minimum inhibitory concentration tests.
Oitenta amostras de Salmonella Enteritidis isoladas de carcaças de frango no período entre maio de 1995 a abril de 1996 no Estado do Rio Grande do Sul, Brasil foram testados para susceptibilidade antimicrobiana pelo método de antibiograma. O antibiograma das amostras apresentou 100 por cento de resistência a colistina, novobiocina, eritromicina e tetraciclina. Tiveram resistência em diferentes níveis a canamicina (1,25 por cento), enrofloxacina (3,75 por cento), neomicina (3,75 por cento), fosfomicina (20 por cento), sulfonamida (86,25 por cento) e nitrofurantoína (90 por cento) e por outro lado não apresentaram resistência a ciprofloxacina, norfloxacina, gentamicina, polimixina B, sulfametrim e sulfazotrim. A constatação de resistência a antibióticos, inclusive àqueles introduzidos na última década, enfatiza a necessidade de uso responsável de antibióticos, e com base em antibiograma ou concentração inibitória mínima.
Subject(s)
In Vitro Techniques , Poultry , Drug Resistance , Salmonella enteritidis , Methods , Microbial Sensitivity Tests , Sampling StudiesABSTRACT
This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method(IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre-enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.