Immediate graft histological assay, post pig's liver transplantation

PURPOSE: To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs. METHODS: Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3. RESULTS: Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7. CONCLUSIONS: The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfer's cells.

Immediate intra and post-surgery complications of the orthotopic liver transplantation in pigs

Objective: to analyze immediate organic modifications and complications subsequent to orthotopic liver transplantation in pigs. Method: fourteen female pigs, specie Sus domesticus, were utilized in this study, with body mass between 5 and 8 Kg, considering that we evaluated the main immediate intra and post-surgery complications of the liver transplantation. Results: in the donor, we highlighted as the main complication the occurrence of bleedings, considering that this was observed in all procedures. In addition, such situation occurred during surgery involving the receptor, in which the bleeding was also perceived as the most frequent complication. However, it is valid to highlight that the hemodynamic instability was present in 5 procedures involving the receptor. Conclusion: among trans surgery complications we may cite: difficulty of oro-tracheal intubation, intra surgery hemorrhages, hemodynamic and respiratory instability and anesthetic complications. In the immediate post-surgery, thus, we observed as the most frequent complications: hemodynamic instability and small anastomotic bleedings

Objetivo: analisar as modificações orgânicas e complicações imediatas subseqüentes ao transplante hepático ortotópico em suínos. Método: foram utilizados no estudo, 14 porcos fêmeas espécie Sus domesticus, com massa corporal entre 5 e 8 Kg, sendo que avaliou-se as principais complicações intra e pós-operatórias imediatas no transplante hepático. Resultados: no doador, destacou-se a ocorrência de sangramentos como principal complicação, tendo em vista que esta foi observada em todos os procedimentos. Tal situação também ocorreu no ato cirúrgico envolvendo o receptor, no qual se percebeu o sangramento como a complicação, também, mais freqüente. Entretanto é valido destacar a instabilidade hemodinâmica, que esteve presente em 5 procedimentos envolvendo o receptor. Conclusão: dentre as complicações trans operatórias podem ser citadas: dificuldade de intubação oro-traqueal, hemorragias intra operatórias, instabilidade hemodinâmica e respiratória e complicações anestésicas. Já no pós-operatório imediato observamos como complicações mais frequentes: instabilidade hemodinâmica e pequenos sangramentos anastomóticos.