ABSTRACT
O objetivo deste estudo foi comparar o potencial osteogênico das células tronco mesenquimais extraídas da medula óssea (CTM-MO) com as do tecido adiposo (CTM-AD) de cães adultos. As células foram caracterizadas fenotipicamente quanto à expressão de CD29, CD90, CD34 e CD45 e submetidas à diferenciação adipogênica e condrogênica por 21 dias e osteogênica por 7, 14 e 21 dias. Foram constituídos quatro grupos: 1) CTM-MO em meio osteogênico, 2) CTM-MO em meio basal, 3) CTM-AD em meio osteogênico e 4) CTM-AD em meio basal. Aos 7, 14 e 21 dias de diferenciação osteogênica as culturas foram submetidas às avaliações da conversão de MTT em formazan, da atividade da fosfatase alcalina (FA), da síntese de colágeno e de matriz mineralizada, avaliação do número de células por campo e foram quantificados os transcritos gênicos para osterix, sialoproteina óssea (BSP), osteonectina (ON) e osteocalcina (OC). Tanto as células extraídas da medula óssea quanto do tecido adiposo mostraram elevada expressão de marcadores para células tronco e baixa expressão de marcadores de células hematopoiéticas (menor que 2%). Além disso, foram capazes de se diferenciar em osteoblastos, condrócitos e adipócitos. As CTM-AD submetidas à diferenciação osteogênica mostraram maior conversão do MTT em formazan que as CTM-MO, sob mesmas condições aos 7 e 21 dias. O número de células por campo, a atividade da FA, a síntese de colágeno e de matriz mineralizada foram superior nas CTM-AD em diferenciação, em relação às CTM-MO sob as mesmas condições, em todos os tempos estudados. As expressões de osterix, BSP e OC foram predominantemente superiores nas CTM-MO diferenciadas, mas a expressão de ON foi superior nas CTM-AD diferenciadas aos 7, 14 e 21 dias. Conclui-se que as CTM-AD apresentam maior potencial osteogênico que as CTM-MO quando extraídas de cães adultos.(AU)
The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. Four groups were formed: BM-MSC in osteogenic medium (1), BM-MSC in basal medium (2), AT-MSC in osteogenic medium (3) and ATMSC in basal medium (4). On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). Both the cells obtained from bone marrow and those from adipose tissue showed high expression of stem cells markers and low expression of hematopoietic cells markers (lower than 2%). Besides, they were able to differentiate into osteoblasts, chondrocytes and adipocytes. AT-MSC submitted to osteogenic differentiation showed higher MTT conversion in formazan than BM-MSC, under the same conditions on days 7 and 21. The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. Expressions of osterix, BSP and OC were predominantly higher in differentiated BMMSC, however the expression of ON was higher AT-MSC differentiated on days 7, 14 and 21. In conclusion, AT-MSC present higher osteogenic potential than BM-MSC when extracted from adult dogs.(AU)
Subject(s)
Animals , Dogs , Adipose Tissue/cytology , Bone Marrow Cells , Osteogenesis , Stem Cells , Bone RegenerationABSTRACT
Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitro models of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120 pmol) intralesionally following spinal cord injury. Seven days after the toxin administration, kidney, brain, lung, heart, liver, adrenal, muscles, pancreas, spleen, stomach, and intestine were histopathologically investigated. In addition, blood samples collected from the rats were tested for any hematologic or biochemical changes.
Subject(s)
Animals , Rats , Bone Marrow , Calcium Channel Blockers/analysis , Cerebrum/anatomy & histology , Conotoxins/analysis , Wounds and Injuries , RatsABSTRACT
Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitro models of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120 pmol) intralesionally following spinal cord injury. Seven days after the toxin administration, kidney, brain, lung, heart, liver, adrenal, muscles, pancreas, spleen, stomach, and intestine were histopathologically investigated. In addition, blood samples collected from the rats were tested for any hematologic or biochemical changes.
Subject(s)
Animals , Rats , Calcium Channel Blockers/analysis , Conotoxins/analysis , Cerebrum/anatomy & histology , Wounds and Injuries , Bone Marrow , RatsABSTRACT
O objetivo deste estudo foi determinar a frequência e avaliar a influência da idade, sexo e raça na soropositividade anti-Babesia canis, Toxoplasma gondii, Leishmania (L.) chagasi e Neospora caninum, por meio da reação de imunofluorescência indireta (RIFI), em amostras de soros coletadas de cães atendidos em nove clínicas veterinárias particulares do município de Lavras, MG, no período de agosto de 2000 a abril de 2002. De 300 cães, 73,3% foram soropositivos (RIFI > 1:80) para B. canis, e houve um aumento significativo de reagentes (p < 0,05) nos animais adultos se comparados com os jovens. Apenas um cão (0,3%), proveniente do município de Belo Horizonte, apresentou anticorpos anti-L. (L.) chagasi (RIFI > 1:40). Para T. gondii, de 218 cães, 60,7% foram positivos (RIFI > 1:16). Em 228 amostras de soros, 3,1% foram positivas (RIFI > 1:50) para N. caninum. Infecções por B. canis e T. gondii são endêmicas em cães atendidos em clínicas veterinárias particulares em Lavras. Não há evidências de casos autóctones de leishmaniose visceral canina em Lavras. Além disso, a infecção por N. caninum é pouco comum em cães criados na zona urbana do município.
The aim of the present study was to determine the frequency and evaluate the influence of age, sex and breed in seropositivity anti-Babesia canis, Toxoplasma gondii, Leishmania (L.) chagasi and Neospora caninum, by means of the indirect immunofluorescence antibody test (IFAT), in serum samples collected from dogs attended in nine private veterinary clinics in municipality of Lavras, Minas Gerais, Brazil, from August 2000 to April 2002. Of 300 dogs, 73.3% were seropositive (IFAT > 1:80) to B. canis, and there was a significant increase (p < 0.05) of the reagent in adult animals when compared with young. Only one dog (0.3%) from Belo Horizonte there was antibodies anti-L. (L.) chagasi (IFAT > 1:40). T. gondii, of 218 dogs, 60.7% were positive (IFAT > 1:16). In 228 serum samples, 3.1% were positive (IFAT > 1:50) to N. caninum. Infections to B. canis and T. gondii occur as endemic form in dogs examined at private veterinary clinics in Lavras. There is no evidence that there are autochthonous cases of canine visceral leishmaniosis in Lavras. Besides this the infection by N. caninum is uncommon in dogs breed at the urbane zone of the municipality.
Subject(s)
Animals , Dogs , Female , Male , Antibodies, Protozoan/blood , Babesia/immunology , Leishmania/immunology , Neospora/immunology , Toxoplasma/immunology , Brazil , Hospitals, AnimalABSTRACT
O objetivo deste estudo foi determinar a freqüencia e avaliar a influencia da idade, sexo e raça na soropositividade anti‑Babesia canis, Toxoplasma gondii, Leishmania (L.) chagasi e Neospora caninum, por meio da reação de imunofluorescência indireta (RIFI), em amostras de soros coletadas de cães atendidos em nove clinicas veterinárias particulares do município de Lavras, MG, no período de agosto de 2000 a abril de 2002. De 300 cães, 73,3% foram soropositivos (RIFI ≥ 1:80) para B. canis, e houve um aumento significativo de reagentes (p < 0,05) nos animais adultos se comparados com os jovens. Apenas um cão (0,3%), proveniente do município de Belo Horizonte, apresentou anticorpos anti-L. (L.) chagasi (RIFI ≥ 1:40). Para T. gondii, de 218 cães, 60,7% foram positivos (RIFI ≥ 1:16). Em 228 amostras de soros, 3,1% foram positivas (RIFI ≥ 1:50) para N. caninum. Infecções por B. canis e T. gondii são endêmicas em cães atendidos em clínicas veterinárias particulares em Lavras. Nao há evidências de casos autóctones de leishmaniose visceral canina em Lavras. Além disso, a infecção por N. caninum e pouco comum em cães criados na zona urbana do município.
The aim of the present study was to determine the frequency and evaluate the influence of age, sex and breed in seropositivity anti-Babesia canis, Toxoplasma gondii, Leishmania (L.) chagasi and Neospora caninum, by means of the indirect immunofluorescence antibody test (IFAT), in serum samples collected from dogs attended in nine private veterinary clinics in municipality of Lavras, Minas Gerais, Brazil, from August 2000 to April 2002. Of 300 dogs, 73.3% were seropositive (IFAT ≥ 1:80) to B. canis, and there was a significant increase (p < 0.05) of the reagent in adult animals when compared with young. Only one dog (0.3%) from Belo Horizonte there was antibodies anti-L. (L.) chagasi (IFAT ≥ 1:40). T. gondii, of 218 dogs, 60.7% were positive (IFAT ≥ 1:16). In 228 serum samples, 3.1% were positive (IFAT ≥1:50) to N. caninum. Infections to B. canis and T. gondii occur as endemic form in dogs examined at private veterinary clinics in Lavras. There is no evidence that there are autochthonous cases of canine visceral leishmaniosis in Lavras. Besides this the infection by N. caninum is uncommon in dogs breed at the urbane zone of the municipality.