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1.
Acta ortop. mex ; 31(6): 283-286, nov.-dic. 2017. graf
Article in Spanish | LILACS | ID: biblio-949781

ABSTRACT

Resumen: Antecedentes: La osteoartritis de rodilla es una enfermedad articular caracterizada por degeneración, pérdida del cartílago y alteración del hueso subcondral asociadas a cambios en los tejidos blandos. En una muestra de 2,500 individuos se encontró una prevalencia de artrosis de 2.3% (IC 95% 1.7 a 2.9) en adultos. En nuestro hospital representa la segunda causa de consulta. Material y métodos: Se tomaron dos grupos de pacientes: el primero, de 70 pacientes a los que se les aplicó hilano G-F 20 en tres infiltraciones; al otro grupo, de 40 pacientes, se le aplicó colágeno-PVP en cuatro aplicaciones. Se les realizó un interrogatorio basado en el cuestionario de WOMAC, con el cual pudimos valorar dolor, rigidez y capacidad funcional antes y después de la aplicación de los medicamentos. Resultados: Nuestro estudio presentó que en ambos grupos hubo mejora de la calidad de vida al disminuir los síntomas tales como dolor, rigidez y limitación funcional; el colágeno fue más eficiente para la disminución del dolor y el hialino G-F 20, para la disminución de la rigidez y la limitación funcional. Conclusiones: Ambos tratamientos mostraron seguridad y eficacia. Su uso en nuestro instituto para los pacientes fuera de tratamiento quirúrgico puede disminuir de manera importante los internamientos por dolor, con lo que podemos hacer un uso más eficiente de los recursos con que contamos.


Abstract: Background: Knee osteoarthritis is a joint disease characterized by degeneration, loss of cartilage and subchondral bone alteration associated with changes in the soft tissues. In a sample of 2,500 individuals, the prevalence of osteoarthritis was found in 2.3% (IC 95% 1.7 a 2.9) in adults; it represents the second cause of consultation in our hospital. Material and methods: Two groups of patients were studied: the first included 70 patients who received hylan G-F 20 in three infiltrations; the other group, of 40 patients, received collagen-PVP in four applications. A subjective questionnaire based on the WOMAC evaluation was applied, with which we could assess pain, stiffness and functional capacity before and after the application. Results: Both hylan G-F 20 and collagen-PVP improved the quality of life of our patients by reducing symptoms such as pain, stiffness and functional limitation, collagen being more efficient for reducing pain and hylan G-F 20 for reducing stiffness and functional limitation. Conclusions: Both treatments were safe and effective; they can be used in our institute as a conservative treatment to decrease hospitalizations for pain management.


Subject(s)
Humans , Adult , Collagen/therapeutic use , Povidone/therapeutic use , Osteoarthritis, Knee/drug therapy , Hyaluronic Acid/analogs & derivatives , Quality of Life , Treatment Outcome , Hyaluronic Acid/therapeutic use , Injections, Intra-Articular
3.
Rev. gastroenterol. Perú ; 26(2): 148-154, abr.-jun. 2006. ilus, tab
Article in Spanish | LILACS, LIPECS | ID: lil-533790

ABSTRACT

Introducción: La anastomosis pancreatoyeyunal es considerada como el punto débil al realizar una duodenopancreatectomia, siendo responsable de la mayoría de las complicaciones luego de realizar una cirugía de Whipple. Aquí presentamos una serie de casos con una misma técnica quirúrgica para efectuar esta anastomosis. Material y método: Durante el periodo comprendido entre Octubre del 2002 a Agosto del 2005 se realizaron 49 duodenopacreatectomías en el servicio 3AII del H.N.E.RM. de las cuales a 31 se les realizó una anastomosis pancreato yeyunal término lateral con técnica mucosa mucosa, por el mismo cirujano. Resultados: La complicación más frecuente fue la infección de la herida operatoria, seguida de la fístula pancreática y la hemorragia intra abdominal, teniendo una morbilidad global de 29 por ciento, La fístula pancreática se presentó en 13 por ciento de los casos no requiriendo tratamiento complementario en ningún paciente, cerrando espontáneamente como máximo a los 20 días del acto quirúrgico. El reinicio de la vía oral fue a los 6 días en promedio y la estancia hospitalaria post-operatoria fue de 16 días. La mortalidad fue de 3 por ciento la cual se presentó en un paciente que desarrollo un pseudoaneurisma de la arteria hepática que se rompió a los 17 días de operado. Conclusiones: La anastomosis pancreatoyeyunal mucosa mucosa es una técnica segura con bajo índice de fístula pancreática y de mortalidad.


Introduction: The pancreatojejunal anastomosis is considered the weak spotwhen carrying out a duodenopancreatectomy, because it causes most of the complicationsfollowing a Whipple surgery. Here we present a series of cases using asingle technique for performing this anastomosis. MATERIAL AND METHODS.- During the period between October 2002 and August 2005, 49 duodenopancreatectomies were performed at the 3AII Department of theÑational Hospital Edgardo Rebagliati Martins - H.N.E.R.M.,in 31 of these cases a lateral mucosa to mucosa pancreatojejunal anastomosis was carried out by the same surgeon.RESULTS.- The most frequent complication was infection of the operating wound followed by pancreatic fistula and intra-abdominal hemorrhage and the overall morbidity was 29 percent. Pancreatic fistula developed in 13 percent of the cases; however, nopatient required additional treatment and the fistula closed maximum twenty days after the surgery. On average, patients resumed oral food intake after 6 days and remained hospitalizedfor 16 days. Mortality was 3 percent, because a patient developed a pseudo-aneurism of the hepatic artery, which ruptured 17 days after the operation.CONCLUSIONS.- The mucosa to mucosa pancreatojejunal anastomosis is a safe technique with a low index of pancreatic fistula and mortality.


Subject(s)
Humans , Male , Female , Anastomosis, Surgical , Pancreatic Fistula , Intestinal Mucosa , Pancreaticoduodenectomy , Pancreas
4.
Rev. biol. trop ; 53(supl.3): 329-336, dic. 2005. tab
Article in Spanish | LILACS | ID: lil-454833

ABSTRACT

Fertilized eggs from an spontaneously spawn of thirty sexually mature sea urchins (Arbacia punctulata) were incubated to complete embryonic development. The echinopluteus larvae (3 ind/ml) were distributed into 50 plastic containers (25 containers at 30 psu and 25 containers at 40 psu) and fed on Tetraselmis chuii, Nannochloropsis oculata, Isochrysis galbana, Chaetoceros gracilis and C. calcitrans under a natural photoperiod. The water of the containers was partially renewed (75%) everyday. Larval anatomic development aspects, daily survival and growth were determined. The growth was determined through postoral arms and body length measurement, and body diameter of twelve larvae during metamorphosis. During the planktonic larval phase, only the I. galbana diet produced similar results for both salinities. The relative growth of larvae was isometric (I) for larvae fed on I. galbana at two salinities and positive allometric for those fed on C. gracilis and C. calcitrans at both salinities. In this study A. punctulata started metamorphosis at day 14 and was completed 30 days after fecundation. Significant differences were detected in post-settlement body growth between the two salinities (F = 23.58, p < 0.05): growth was better for larvae at 30 psu (final body diameter was 3.14 +/- 0.44 mm). The final rate of planktonic larvae was highest with I. galbana (58.33%). For juveniles the rate was 6.48% for those fed on C. gracilis (40 psu in both larvae and juveniles). We recommend the use of this diet and 40 psu for survival or 30 psu for growth.


Subject(s)
Animals , Female , Animal Feed , Eukaryota , Ovum/drug effects , Sea Urchins/growth & development , Sodium Chloride/pharmacology , Aquaculture , Cells, Cultured , Larva/drug effects , Larva/growth & development , Ovum/growth & development , Survival Rate
5.
Rev. biol. trop ; 53(supl.3): 337-344, dic. 2005. tab
Article in Spanish | LILACS | ID: lil-454834

ABSTRACT

Thirty sexually mature sea urchins (Echinometra lucunter; diameter 45.8 +/- 17.5 mm) were collected at Macanao, Margarita Island, Venezuela (11 degrees 48'29" N / 64 degrees 13'10" W). They were injected potassium chloride (50 M) directly into the celomic cavity. After two minutes 90% spawned (17 females and 10 males), the others never spawned. Fertilization was 87.0 +/- 12.6% (1:100 oocytes/sperm) at 29 +/- 2 degrees C. The fertile eggs were placed in three treatment gropsu with nine containers (18 liters; 2 eggs/ml) each, all with bottom aeration. Treatments were: Chaetoceros gracilis; Isochrysis galbana, and a mixture of both microalgae (respectively: 20 000 and 60,000 cell/ml for each microalgae, 1:1 for the mixture). Salinity, pH, temperature and larval survival were determinated daily. The study ended when the post-metamorphic phase was completed. The embryonic development time was 16.3 +/- 0.2 h until the prism stage at pH 8.4 +/- 0.1; 38 +/- 1 psu and 28 +/- 1.4 degrees C. The two-arms larval stage was reached at 24 h: 33 min, with a total length of 190 +/- 16.3 microm fed on C. gracilis, 152 +/- 19.0 microm with I. galbana and 182.4 +/- 14.1 microm with the mixture. The larvae next to metamorphosis reabsorbed the arms and had the characteristic shape of juvenile urchins at 12 days with 670.2 +/- 22.2 microm fed on C. gracilis, 665 +/- 12.1 microm fed on I. galbana and 670 +/- 14.1 microm fed on the mixture. The accumulated survival to the juvenile stage was 14.7 +/- 3.8% when fed on C. gracilis, higher than the other treatments (5.4 +/- 1.2; 14.0 +/- 2.6). E. lucunter is an excellent prospect to be commercially cultured because of its short embryonic (16 hours) and larval development time (12 days) and good survival rate when fed on monoculture (C. gracilis and I. galbana) or mixed diet (we recommend C. gracilis)


Subject(s)
Animals , Male , Female , Animal Feed , Eukaryota , Ovum/growth & development , Sea Urchins/growth & development , Sodium Chloride/pharmacology , Aquaculture , Cells, Cultured , Hydrogen-Ion Concentration , Larva/drug effects , Larva/growth & development , Metamorphosis, Biological , Ovum/drug effects , Survival Analysis , Sea Urchins/embryology
7.
Microsc. electron. biol. celular ; 14(2): 165-71, 1990.
Article in English | LILACS | ID: lil-121629

ABSTRACT

In order to increase the value of the zona-free hamster oocyte penetration test, a comparetively simple and fast method using the fluorochrome Hoechst 33342 was developed. human spermatozoa were washed and incubated 1 hr mediumBWW for capacitation. hamster oocytes were stripped of cumulus oophorus and zona pellucida with hyaluronidase and trypson, washed and used immediately. Thirthy oocytes were placed in a drop of BWW containing 3,5.10(6)/ml of human spermazoa under mineral oil. The sperm-oocyte preparation was incubated for 3 hr at 37 degrees C, during the last 15 min of incubation, the fluorochrome Hoechst 33342 (H) was added and incubation was allowed to proceed until the incubation time was over. Observations showed that the female pronucleus, eccentrically placed, gives a bright green-bluish fluorescence whereas chromatin of sperm heads shows different stages of decondensation and also a bright fluorescence. This inexpensive method has given consistent results in a large number of cases and provides an additional new approach to the <> as a proof of the capacity to form a <>


Subject(s)
Humans , Animals , Male , Female , Cricetinae , Benzimidazoles , DNA/analysis , Fluorescent Dyes , Oocytes/ultrastructure , Sperm-Ovum Interactions , Spermatozoa/ultrastructure , Staining and Labeling , Infertility, Male/diagnosis , Mesocricetus , Cell Nucleus/ultrastructure , Spermatozoa/chemistry , Spermatozoa/physiology
8.
Microsc. electron. biol. celular ; 11(1): 67-79, Jun. 1987. ilus
Article in English | LILACS | ID: lil-75577

ABSTRACT

Los cambios que experimenta la cabeza del espermatozoide de hamster durante su tránsito por el epidídimo fueron analizados con técnicas morfológicas, de criofractura y morfométricas. Desde el punto de vista morfológico se comprueba una modificación del ángulo formado por el acrosoma y la región postacrosomal que de recto en espermatozoides del caput pasa a obtuso en espermatozoides de cauda. En cuanto a la criofractura se estudió la distribución de las partículas intramembranosas en la membrana plamática (IMPS). Se describe una disposición regular de las IMPS formando una trama hexagonal en el borde convexo del acrosoma. Esta trama está presente en espermatozoides extraídos del caput y no se observa en los extraídos de la región caudal del epidídimo. La región postacrosomal revela dos cambios notables: la presencia de una corona de barras adyacentes al cuello y formada por IMPS de 10 nm en espermatozoides del caput que se desorganiza en espermatozoides extraídos del epidídimo caudal y el incremento en estos del número de IMPS de 10 nm dispersas por toda la región postacrosomal. Postulamos que el aumento de IMPS en la región postacrosomal se relaciona con la incorporación de glicoproteínas secretadas por epidídimo como factor (es) de maduración y reconocimiento intergamético


Subject(s)
Cricetinae , Animals , Male , Epididymis/ultrastructure , Sperm Maturation , Sperm Transport , Spermatozoa/ultrastructure
9.
Prensa méd. argent ; 70(1): 28-31, 1983.
Article in Spanish | LILACS | ID: lil-14929
10.
Rev. argent. cir ; 42(6): 288-90, 1982.
Article in Spanish | LILACS | ID: lil-9473

ABSTRACT

Con el proposito de valorar la utilizacion de homoinjertos vasculares, aorticos y cavales, mantenidos en gluteraldehido, para reemplazar el segmento suprarrenal de la vena cava inferior, fueron empleados 55 especimenes caninos a los que se les realizaron estudios de laboratorio, flebograficos y anatomopatologicos. Se observo obstruccion precoz y muerte rapida de los animales cuando se utilizo aorta, y sobrevida de los mismos, con estenosis y oclusion tardia, con desarrollo de circulacion colateral, cuando fue colocado el homoinjerto venoso


Subject(s)
Male , Female , Animals , Dogs , Glutaral , Tissue Preservation , Transplantation, Homologous , Vascular Surgical Procedures , Vena Cava, Inferior
13.
Rev. argent. cir ; 40(6): 295-300, 1981.
Article in Spanish | LILACS | ID: lil-3754

ABSTRACT

Con el proposito de valorar experimentalmente el aislamiento vascular del higado in situ, interrumpiendo el pediculo aferente (vena porta y arteria hepatica) y el eferente (vena cava inferior retrohepatica) para ser aplicado especialmente en las lesiones traumaticas de este organo, que se acompanan de rupturas venosas suprahepaticas o cavales, se utilizaron 83 animales, analizandose los resultados a traves de la histopatologia, el laboratorio, los controles hemodinamicos y clinicos.Estos evidenciaron claramente las ventajas de la utilizacion de un cortocircuito interno, a traves de un tubo intracava, introducido por via femoral, para mantener el retorno venoso, ante una situacion de "shock" hemorragico. La hipotermia del organo in situ, por medio de la perfusion de soluciones electroliticas enfriadas, demostro real eficacia


Subject(s)
Abdominal Injuries , Liver Regeneration , Vascular Surgical Procedures
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