ABSTRACT
Objective To evaluate the feasibility and efficacy of autologous splenic segment implantation after splenectomy for traumatic rupture. Methods This study included 42 patients with traumatic splenic rupture who were treated between July 2014 and August 2016 at the Affiliated Central Hospital of Shenyang Medical College. The patients were divided into an observation group (n = 23) and a control group (n = 19). Informed consent was provided by the patient or family members. The control group underwent routine splenectomy, and the observation group underwent implantation of autologous spleen segments after splenectomy. Results The operative time in the observation group was significantly longer than in the control group (P < 0.05). The intraoperative blood loss,time to postoperative return to oral feeding,and length of hospital stay were not statistically different (P > 0.05). The rate of postoperative wound infections in the observation group was significantly lower than in the control group (P < 0.05). All spleen segments developed well in the observation group. At 30 postoperative days,platelets were increased in both groups,but the level in the observation group was significantly lower than in the control group (P < 0.05). IgG,IgM,and IgA levels were significantly higher in the observation group than in the control group at 28 postoperative days (P < 0.01). CD3+ and CD4+/CD8+ levels were significantly higher in the observation group than in the control group at 14 and 28 postoperative days (P < 0.01). Conclusion Implantation of autologous spleen segments after splenectomy for trauma could be feasible and effective.
ABSTRACT
Objective To investigate whether pancreatic islet transplantation in combination with bone mesenchymal stem cells (MSC) transplantation can promote the vascularization surrounding the transplant pancreatic islet.Methods The non-obese diabetic (NOD) mice were utilized as the recipients and randomly divided into pancreatic islet transplantation combined with MSC transplantation group (co-transplantation group,n=6),pancreatic islet transplantation group(n=6),MSC transplantation group(n=6) and sham transplantation group (n=3).The variation in blood glucose level and survival rate post-transplantation of NOD mice in each group was observed.The proliferation and apoptosis of the transplant pancreatic islet in the pancreatic islet transplantation group and co-transplantation group at 1,2 and 4 weeks after pancreatic islet transplantation were analyzed by 5-ethynyl-2'-deoxyufidine (EdU) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).The vascular density surrounding the transplanted pancreatic islet in the pancreatic islet transplantation group and co-transplantation group at postoperative 2,4 and 8 weeks were observed under light microscope and quantitatively analyzed by histochemical and immunohistochemical staining.Results Both MSC combined with pancreatic islet transplantation and pancreatic islet transplantation significantly improved the blood glucose level and enhanced the survival rate of NOD mouse models after transplantation.In addition,it could accelerate the regeneration of pancreatic islet cells and decrease cell apoptosis.MSC combined with pancreatic islet transplantation significantly enhanced the vascular density surrounding the transplant pancreatic islet compared with pancreatic islet transplantation alone.Conclusions MSC transplantation can accelerate the vascularization surrounding the transplant pancreatic islet,increase the blood supply and protect the function and activity of the transplant pancreatic islet.