Research and exploration of salivary biological markers for hepatitis B-related hepatocellular carcinoma / 中华肝脏病杂志

Objective: To study using isotope-labeled relative and absolute quantitative proteomics methodologies to screen for salivary biological markers as a simple, non-invasive tool for identifying hepatitis B-related HCC at an early stage. Methods: Saliva samples were collected to extract salivary proteins. Isotope-labeled relative and absolute quantitative proteomics were used to analyze the differentially expressed proteins between the hepatocellular carcinoma (HCC) and non-HCC groups. Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays were used to verify differential proteins and identify markers in liver cancer tissues and saliva. Statistical analysis was used to analyze the diagnostic efficiency of salivary biomarkers. Results: 152 differentially expressed salivary proteins were screened out between the HCC and non-HCC groups. Western blot, immunohistochemistry, and enzyme-linked immunosorbent assays validated that the expressions of α-1-acid glycoprotein 1 (ORM1) and alpha-fetoprotein (AFP) were significantly increased in HCC (P < 0.05). There was a significant correlation between salivary AFP and serum AFP (P < 0.05). HCC was diagnosed when salivary α-1-acid glycoprotein 1 combined with AFP. The area under the receiver operating characteristic curve was 0.8726 (95% confidence interval: 0.8104 ~ 0.9347), the sensitivity was 78.3%, and the specificity was 88%. Conclusion: Salivary AFP and α-1-acid glycoprotein 1 can serve as potential biomarkers for hepatitis B-related hepatocellular carcinoma.

Clinicopatholigical differences between patients with adenocarcinoma of esophagogastric junction and gastric cancer without esophagogastric junction invasion / 国际外科学杂志

Objective To investigate the clinicopatholigical differences between patients with adenocarcinoma of esophagogastric junction (AEG) and gastric cancer without esophagogastric junction invasion,and relationship between the two type cancers and Helicobacter pylori (Hp) infection,body mass index.Methods A retrospective analysis was performed on 577 cases of gastric cancer patientsin the Third Hospital of Mianyang from December 2013 to February 2018.Patients were divided into AEG group (n =218) and gastric cancer without esophagogastric junction invasion group (n =359) according to location of neoplasm.The age,gender,body mass index,Hp infection,pathological stage,operative type and blood loss in operation of patients between two groups were compared,and the relationship between the two type cancers and Hp infection,body mass index was analyzed by logistic regression,OR and 95％ CI:was calculated.Results AEG patients were accounted for 37.8％,and had a relatively higher percentage of males,older age,more open surgery,surgery duration and perioperative transfusion,and a relatively lower percentage of Hp infection and family history of gastric cancers.When adjusted for age,gender and family history,the frequency of infection with Hp was higher in patients with gastric cancer without esophagogastric junction invasion,the OR was 1.73 (95％ CI:1.22-2.46,P ＜ 0.05),while the differences of BMI was not significant between patents in two groups.Conclusion The different characteristics of AEG and gastric cancer without esophagogastric junction invasion cases have implications for the development of guiding strategies for diagnosis and treatment of gastric cancer patients based on tumor location.

Clinicopatholigical differences between patients with adenocarcinoma of esophagogastric junction and gastric cancer without esophagogastric junction invasion / 国际外科学杂志

Objective@#To investigate the clinicopatholigical differences between patients with adenocarcinoma of esophagogastric junction (AEG) and gastric cancer without esophagogastric junction invasion, and relationship between the two type cancers and Helicobacter pylori (Hp) infection, body mass index.@*Methods@#A retrospective analysis was performed on 577 cases of gastric cancer patientsin the Third Hospital of Mianyang from December 2013 to February 2018.Patients were divided into AEG group (n=218) and gastric cancer without esophagogastric junction invasion group (n=359) according to location of neoplasm. The age, gender, body mass index, Hp infection, pathological stage, operative type and blood loss in operation of patients between two groups were compared, and the relationship between the two type cancers and Hp infection, body mass index was analyzed by logistic regression, OR and 95%CI: was calculated.@*Results@#AEG patients were accounted for 37.8%, and had a relatively higher percentage of males, older age, more open surgery, surgery duration and perioperative transfusion, and a relatively lower percentage of Hp infection and family history of gastric cancers. When adjusted for age, gender and family history, the frequency of infection with Hp was higher in patients with gastric cancer without esophagogastric junction invasion, the OR was 1.73(95%CI: 1.22-2.46, P<0.05), while the differences of BMI was not significant between patents in two groups.@*Conclusion@#The different characteristics of AEG and gastric cancer without esophagogastric junction invasion cases have implications for the development of guiding strategies for diagnosis and treatment of gastric cancer patients based on tumor location.

Effect of parent training in combination with methylphenidate treatment on family relationships for children with attention deficit/hyperactivity disorder / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the effect of parent training combined with methylphenidate treatment on family relationships in children with attention deficit/hyperactivity disorder (ADHD).</p><p><b>METHODS</b>Fifty-nine parents of children with ADHD under methylphenidate treatment participated in a modified 5-week training program. The intervention effect was evaluated using the Conners Parent Symptom Questionnaire, ADHD Rating Scale-IV Home Version (ADHD-RS-IV Home Version), Caregiver Strain Questionnaire, Parent-Child Relationship Self-rating Scale and Piers-Harris Children's Self-Concept Scale. Parents also completed the training satisfaction survey before and after the intervention.</p><p><b>RESULTS</b>After the 5-week parent training, compared with the baseline values, total scores of Conners Parent Symptom Questionnaire and scores of conduct problems and anxiety significantly decreased, and scores of attention deficit, hyperactivity, impulsivity and oppositional defiant behaviors of ADHD-RS-IV Home Version, and Caregiver Strain Questionnaire total scores were all significantly decreased (P<0.05), while total scores of the Parent-Child Relationship Self-Rating Scale and Piers-Harris Children's Self-Concept Scale were significantly increased (P<0.05).</p><p><b>CONCLUSIONS</b>Modified 5-week parent training program may improve parent-child relationship and reduce parenting stress in ADHD families.</p>

Study on attention-deficit hyperactivity disorder in children based on resting-state functional magnetic resonance imaging / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To compare resting-state functional magnetic resonance imaging (fMRI) findings of children with attention-deficit hyperactivity disorder (ADHD) and normal children, and to investigate the possible mechanism of brain dysfunction in children with ADHD.</p><p><b>METHODS</b>Resting-state fMRI was performed on 18 children who met the DSM-IV diagnostic criteria for ADHD (ADHD group) and 18 normal children (control group) matched for age, sex, IQ, degree of education and handedness. The two groups were compared in terms of amplitude of low frequency fluctuation (ALFF) and regional homogeneity (ReHo).</p><p><b>RESULTS</b>Compared with the control group, the ADHD group had decreased ALFF in the bilateral posterior lobes of the cerebellum and the left side of the pons, increased ALFF in the right precentral gyrus, decreased ReHo in the left medial frontal gyrus, right superior frontal gyrus, and left precuneus, and increased ReHo in the left anterior lobe of the cerebellum, left caudate nucleus, right parahippocampal gyrus, left precentral gyrus, and right middle frontal gyrus.</p><p><b>CONCLUSIONS</b>In resting state, children with ADHD have decreased brain activity in some regions, including the cerebellum and frontal cortex, compared with normal children, which supports the hypothesis of dysfunctional fronto-cerebellar circuits in ADHD.</p>

The associated research on the expression of RUNX3 mRNA and protein in hepatic cell carcinoma as well as the relationship with clinical pathological parameters / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To investigate the expression of RUNX3 mRNA and protein in hepatic cell carcinoma (HCC) and surrounding normal tissue, to analyze the relationship between RUNX3 expression and clinical pathological parameters.</p><p><b>METHODS</b>Reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) were performed to detect the expression of RUNX3 mRNA and protein in HCC and surrounding normal tissue respectively, and their relationship with clinical pathological parameters were analyzed.</p><p><b>RESULTS</b>The relative expression value of RUNX3 mRNA found in 51 cases HCC was 0.4509 +/- 0.0963, and that did in 51 cases surrounding normal tissue was 0.9147 +/- 0.0222. The difference of RUNX3 mRNA expression between two kinds of samples was statistically significant (t = 33.6087, P < 0.001). The positives rate of RUNX3 protein expression found in 51 cases HCC tissue was 49.02% (25/51) and that did in 51 cases surrounding normal tissue was 82.35% (42/51). The difference of RUNX3 protein expression between two kinds of samples was statistically significant (chi2 = 12.5706, P < 0.005). The difference of RUNX3 mRNA and protein expression in some clinical pathological parameters involving differentiation degree, invasion, cancer thrombus and diversion in liver were statistically significant (P < 0.05). However that were not in another clinical pathological parameters involving gender, cancer diameter, cancer location as well as hemorrhage and necrosis of cancer, histotype (P > 0.05).</p><p><b>CONCLUSION</b>The expression of RUNX3 mRNA and protein in HCC were significantly lower than that in surrounding normal tissue. The lower expression of runx3 protein in the HCC probably plays an important role in the tumorigenesis and development of HCC. The RUNX3 gene may be an anti-oncogene of HCC.</p>

Expression and significance of liver X receptor-alpha in nonalcoholic fatty liver disease in rats / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>Preparing rat model of non-alcoholic fatty liver disease by fat-rich diet to observe the expression and the role of LXR-alpha in rat nonalcoholic fatty liver disease.</p><p><b>METHODS</b>Thirty-six SD rats were randonmized into basic diet-control group and high-fat diet group. Each of the two groups was subdivided into 3 subgroups (4, 8, 12 weeks). Changes in animal weight, liver exponent and the level of TG and TC in serum and liver were observed dynamically. Meanwhile,the expression of hepatocyte LXR-alpha and SREBP-1c were assayed by Reverse transcript-polymerase chain reaction at 4, 8, 12 weeks. The level of steatosis was observed under light microscope after haematoxylon-eosin (HE) staening.</p><p><b>RESULTS</b>Compared with control group, body weight, liver exponent, TG and Tc in serum and liver were increased dynamically in model groups. Compared with control group, the mRNA of LXR-a and SREBP-1c were obviously increased dynamically in model groups (P < 0.05) .</p><p><b>CONCLUSION</b>The increase of LXR-alpha and SREBP-1c in liver may be concered with energy disorder and closely associated with the activity of inflammation and the severity of the liver damage in NAFLD rats.</p>

Effects of interferon on hepatic intercellular adhesion molecule-1 expression in patients with chronic hepatitis B / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effects of interferon -alpha1b (IFN-alpha1b) on hepatic intercellular adhesion molecule-1 (ICAM-1) expression and serum HBV DNA in patients with chronic hepatitis B.</p><p><b>METHODS</b>Before and 6 months after IFN-alpha1b treatment, liver biopsy was performed in patients with chronic hepatitis B to detect the expression of ICAM-1 in the liver tissues using immunohistochemistry. Serum HBV load was detected with real-time fluorescence polymerase chain reaction.</p><p><b>RESULT</b>CAM-1 expression in the liver tissue was significantly down-regulated after IFN treatment in patients with severe and moderate chronic hepatitis B (P<0.05). No significant variation was noted in the expression of ICAM-1 in the livers of patients with mild chronic hepatitis B after the treatment (P>0.05). In the patients weakly positive for ICAM-1 expression (+), serum HBV DNA varied scarcely after the treatment (P>0.05), while in the patients with strong ICAM-1 positivity (++, +++, or ++++), significant variation of serum HBV DNA occurred after the treatment (P<0.05 or P<0.01).</p><p><b>CONCLUSION</b>The therapeutic effect of IFN-alpha1b is associated with the expression of ICAM-1 in the hepatocytes, and its expression might enhance the effects of IFN on HBV DNA in patients with chronic hepatitis B.</p>

The effects of Shuxuetong on the pathology of cerebral ischemia-reperfusion injury and GABA and TNF-alpha expression in gerbil models / 神经科学通报·英文版

Objective To study the protective effect and mechanism of Shuxuetong on gerbil brain tissue from the area of ischemia-reperfusion. Methods Cerebral ischemia-reperfusion animal model has made by transient clipping bilateral common carotid arteries in gerbils. Pathological changes in the hippocampal tissue were observed at different reperfusion time (12h, 3 d, 7 d). The expression levels of GABA and TNF-alpha in the hippocampal CA1 subfield were observed using immunohistochemitry at 12 h, 3 d after reperfusion. The difference of above indices among false operation group, ischemia-reperfusion group and treatment group were compared. Results The injuries of pathology to hippocampal area in ischemia reperfusion group were more serious than treatment group. The expression levels of GABA in treatment group were significantly increased compared with ischemia-reperfusion group, but the expression levels of TNF-alpha between the two groups have no difference. Conclusion Shuxuetong has protective effect on brain tissue of ischemia-reperfusion by enhancing the expression of GABA in the hippocampal tissue.

A clinical and pathological study of nonalcoholic fatty liver disease / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To study the pathological and clinical features of nonalcoholic fatty liver disease (NAFLD).</p><p><b>METHODS</b>Grades and stages of liver lesions in 41 patients with NAFLD were analyzed. The relationships between pathohistological features of the livers, serum biochemical parameters, ultrasound examination and other clinical data of the patients were studied.</p><p><b>RESULTS</b>Among the 41 patients with NAFLD (there were 21 with their liver fatty degeneration in grade 1, 15 in grade 2, and 5 in grade 3). There were 2 of grade 0, grade 1 had 25, grade 2 had 10, grade 3 had 3, and grade 4 had 1. Stage 0 of fibrosis was 20, stage 1 was 14, stage 2 was 4, stage 3 was 2, and stage 4 was 1. Degree of fatty degeneration was not positively associated with the body mass index (BMI) of the patients and the ultrasound findings in their livers. Grading of the inflammation was positively related to the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the blood and ultrasound findings in their livers, but negatively to the platelet counts. Staging of fibrosis of the livers was positively related to the blood ALT, AST, GGT, and ALP, and negatively to triglyceride levels and platelet counts.</p><p><b>CONCLUSIONS</b>Degree of liver fatty degeneration was not associated with grades of inflammation and staging of fibrosis of the liver. BMI, ALT and AST level, platelet counts, and ultrasound grades of fatty liver were associated with the liver histopathological changes of NAFLD patients. Liver biopsy is the essential way to make a diagnosis of NAFLD.</p>

Histological study of livers from the patients with chronic hepatitis B treated with bicyclol / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To study histological changes of the livers in patients with chronic viral hepatitis B treated with bicyclol tablets.</p><p><b>METHODS</b>Thirty one patients with chronic viral hepatitis B were divided into two groups and were treated with bicyclol orally at doses of 150 mg daily or 75 mg daily for 36 weeds. The histological changes of the livers were observed before and after the treatment.</p><p><b>RESULTS</b>Compared with pre-treatment findings, there were significant differences in histological activity index in each group (P < 0.01, P < 0.05), there were also significant differences between the two groups (P < 0.05). Decreased inflammatory reaction was also seen (P < 0.05).</p><p><b>CONCLUSION</b>Daily use of 150 mg and 75 mg bicyclol tablets are effective in improving liver histological changes in chronic hepatitis B patients. Bicyclol 150 mg daily was better.</p>